Forests, Volume 12, Issue 2 (February 2021) – 150 articles

Aiming to develop a new tree biomass estimation model that is adaptable to airborne observations of forest canopies by unmanned aerial vehicles (UAVs), we applied two theories of plant form; the pipe model theory (PMT) and the statical model of plant form as an extension of the PMT for tall trees. Based on these theories, tree biomass was formulated using an individual tree canopy height model derived from a UAV. The advantage of this model is that it does not depend on diameter at breast height which is difficult to observe using remote-sensing techniques. We also proposed a treetop detection method based on the fractal geometry of the crown and stand. Comparing surveys in plantations of Japanese cedar (Cryptomeria japonica D. Don) and Japanese cypress (Chamaecyparis obtusa Endl.) in Japan, the root mean square error (RMSE) of the estimated stem volume was 0.26 m³ and was smaller than or comparative to that of models using different methodologies. The significance of this model is that it contains only one empirical parameter to be adjusted which was found to be rather stable among different species and sites, suggesting the wide adaptability of the model. Finally, we demonstrated the potential applicability of the model to light detection and ranging (LiDAR) data which can provide vertical leaf density distribution. Full article

To explore the molecular regulatory mechanism of high-carbon (C) sequestration Fraxinus mandshurica Rupr. (F. mandshurica) provenance and the expression profile of F. mandshurica during nitrogen (N) starvation, the foliage and roots of the annual Wuchang (WC) seedlings with greater C amount and Hailin (HL) seedlings with smaller C amount, which were grown in N-deficient nutrition and complete N, were used for RNA-seq and physiological determination, respectively. One thousand and fifty-seven differentially expressed genes (DEGs) between WC and HL and 8173 DEGs related to N deficiency were identified, respectively. The root of F. mandshurica responded to N deficiency more strongly than foliar. The target genes that responded to N deficiency in roots were mainly regulatory genes (transcription factors, hormones and protein kinases), and their response patterns were upregulated. The growth and N concentration in both WC and HL were reduced by the N deficiency, which might result from the decrease of the leaf Nitrate reductase (NR) and glutamine synthetase (GS) enzyme activity and ABA content, although the root-to-shoot ratio; lateral root number; lignin content; endogenous hormones content (GA, IAA and ZR); root GS and glutamate synthetase activity and transcriptional level of most of the regulatory genes were increased. The C sequestration capacity in WC was greater than that in HL, which related to the higher GS enzymes activity and transcriptional levels of regulatory genes and metabolic genes (terpenes, carbohydrates, and lipid energy). However, the C sequestration advantage of WC was significantly reduced by the N deficiency, which was due to the smaller response to N deficiency compared to HL. Full article

Climate change requires forest managers to explore new concepts in reforestation. High-elevation sites are posing challenges because the range of tree species that can cope with present and future conditions is small and limited experience with candidate species is available. Methods: We selected a mountain site with nutrient-poor silicatic soils. The previous Norway spruce (Picea abies) stand performed poorly. We established a reforestation experiment with 27 tree species that were planted in different combinations in order to evaluate silvicultural options. Site preparation activities and planting techniques reflected the locally applied regular procedures. After planting, we monitored height growth and phenological characteristics of needle/leaf development in spring. The presently dominant Norway spruce was genetically characterized. Results: Tree seedlings planted at high elevation are highly vulnerable. The temporal course of needle/leaf sprouting varies widely. Early developers are vulnerable to frost, impairing tree development. Biotic stressors such as high population densities of weevils or mice can cause high mortality. Conclusion: we suggest a conservative approach to tree species selection because present site conditions in mountain areas may impair the development of many tree species that could be viable options in a considerably warmer climate. Full article

Deforestation shows the constant environmental degradation that occurs worldwide as a result of the growth of economic activity and the increase in population. This research examines the causal link between renewable energy consumption, GDP, GDP², non-renewable energy price, population growth and forest area in high, middle- and low-income countries. Based on data obtained from World Development Indicators, the autoregressive distributed lag model, with a time series, is used to examine the long-term cointegration relationship between the variables. The results justify the existence of a joint long-term relationship between the variables analysed for the middle-income countries and low-income countries. When the forest area is not at its equilibrium level, the speed of adjustment is slow (0.44% and 8.7%), which is typical of the nature of this natural resource. An increase in the consumption of renewable energy is associated with an increase between 0.04 and 0.02 square kilometres of forest cover, respectively. The research does not show evidence about the equilibrium relationship in the short term. Growth in renewable energy consumption is one of the main drivers for preserving the forest area. Therefore, those responsible for making economic policies must aim their measures towards the use of clean energy. Full article

Cupressaceae pollen is responsible for winter respiratory allergies in the Mediterranean area. Pollen grains of this diverse family share the same characteristics under light microscopy. Consequently, the partial contribution of each Cupressaceae species to the airborne pollen spectrum cannot be determined with conventional aerobiological methods. Studies on major aeroallergens offer better information on the allergic sensitization and appearance of symptoms in patients sensitized to airborne pollen and spores. Our aim was to determine if airborne Cupressaceae pollen concentrations correspond to aerodynamics of the major allergen of Cupressus (Cup a 1), as well as the aeroallergen correlation with different-sized particles. The air was sampled in two cities of Southern Spain (Granada and Córdoba) using the Hirst-type volumetric sampler for airborne pollen detection during 2006 to 2008 and the Andersen Cascade Impactor for aeroallergen detection during the main pollination period of Cupressaceae in the same period. The samples were analyzed by the methodology proposed by the Spanish Aerobiology Network (REA), the minimum requirements of the European Aeroallergen Society (EAS) for pollen, and by ELISA immunoassay to detect airborne Cup a 1. The distribution patterns of airborne Cupressaceae pollen and Cup a 1 were observed throughout the sampling period, although with some irregular oscillations. Cupressaceae pollen and Cup a 1 showed positive and significant correlation during the period of maximum concentrations (January to March). However, the results of this study showed that the period of exposure of aeroallergens depends on the Cupressus species and their abundance in cities. According to the allergy potential obtained, species like C. arizonica could release more allergens than other species like C. sempervirens. Full article

The accuracy of the description regarding tree architecture is crucial for data processing. LiDAR technology is an efficient solution for capturing the characteristics of individual trees. The aim of the present study was to analyze tree shape variability in a mixed oak forest consisting of four European white oak species: Quercus petraea, Q. frainetto, Q. pubescens, and Q. robur. Moreover, we tested for association between tree shape and individual heterozygosity and whether oak trees identified as pollen donors in a previous genetic study have a larger size in terms of crown and trunk characteristics than non-donors. The woody structure of a tree was defined by the quantitative structure model (QSM) providing information about topology (branching structure), geometry, and volume. For extracting the 3D point clouds a high-speed 3D scanner (FARO FocusS 70) was used. The crown variables were strongly correlated to each other, the branch volume being influenced by branch length, maximum branch order, and the number of branches but not influenced by diameter at breast height (DBH), trunk length, trunk volume, or tree height. There was no relationship between the individual heterozygosity based on nuclear microsatellite genetic markers and crown and trunk characteristics, respectively. Branch volume, total area, DBH, trunk volume, and the total volume of tree were significantly larger in pollen donors compared to non-donor Q. petraea trees. Thus, the mean branch volume was more than three times higher. Pollen donors had nearly two and half times larger total area in comparison to non-donor individuals. Our results suggest that a thorough characterization of tree phenotype using terrestrial laser scanning may contribute to a better understanding of mating system patterns in oak forests. Full article

While the myriad benefits of palm oil as a food, makeup, and cleaning product additive drive its demand, globally, the palm oil industry remains largely unsustainable and unregulated. The negative externalities of palm oil production are diverse and devastating to tropical ecosystem integrity and human livelihoods in palm oil nations. Given the current trend in increasing sustainability and transparency in global supply chains, we suggest that sustainability policy reforms are feasible and have the potential to promote 21st century U.S. and international sustainability standards. Polycentric governance may improve the attainment of sustainable global palm oil standards with a set of rules that interact across linear and nonlinear hierarchies and structures, thereby improving collaboration efforts, and increasing connectivity and learning across scales and cultures. Transformations towards sustainability in international palm oil governance has the potential to make valuable contributions to global sustainable development and improve the prosperity of poor rural communities in the tropics by providing a framework for achieving palm oil trade transparency and aligning the sustainability goals across a range of actors. Full article

Although the radial movement of minerals in tree trunks is a widely accepted phenomenon, experimental evidence of their movement in standing trees and underlying mechanisms is very limited. Previously, we clarified that cesium (Cs) artificially injected into the outer part of the sapwood of standing Japanese cedar (Cryptomeria japonica D. Don) trunks moved to the inner part of the sapwood, including the intermediate wood, via active transport by xylem parenchyma cells and diffusion through cell walls and then moved into the heartwood by diffusion. To understand the mechanism underlying the radial movement of minerals in the standing tree trunk, it is necessary to clarify their movement in the opposite direction. Therefore, the present study aimed to determine the radial movement of minerals from inside to outside in the trunks of standing trees at the cellular level. For this, a long hole across the center part of the trunk, which reached the heartwood, intermediate wood, and sapwood, was made in standing Japanese cedar trunks, and a solution of stable isotope Cs was continuously injected into the hole for several days as a tracer. The injected part of the trunk was collected after being freeze-fixed with liquid nitrogen, and the frozen sample was subjected to analysis of Cs distribution at the cellular level using cryo-scanning electron microscopy/energy-dispersive X-ray spectroscopy. The Cs injected into the inner sapwood or intermediate wood rapidly moved toward the outer sapwood via xylem ray parenchyma cells together with diffusion through the cell walls. In contrast, the Cs injected into the heartwood barely moved to the sapwood, although it reached a part of the inner intermediate wood. These results suggest that minerals in xylem ray parenchyma cells in the sapwood are bidirectionally supplied to each other; however, the minerals accumulated in the heartwood may not be supplied to living cells. Full article

Applications of unmanned aerial systems for forest monitoring are increasing and drive a need to understand how image processing workflows impact end-user products’ accuracy from tree detection methods. Increasing image overlap and making acquisitions at lower altitudes improve how structure from motion point clouds represents forest canopies. However, only limited testing has evaluated how image resolution and point cloud filtering impact the detection of individual tree locations and heights. We evaluate how Agisoft Metashape’s build dense cloud Quality (image resolution) and depth map filter settings influence tree detection from canopy height models in ponderosa pine forests. Finer resolution imagery with minimal filtering provided the best visual representation of vegetation detail for trees of all sizes. These same settings maximized tree detection F-score at >0.72 for overstory (>7 m tall) and >0.60 for understory trees. Additionally, overstory tree height bias and precision improve as image resolution becomes finer. Overstory and understory tree detection in open-canopy conifer systems might be optimized using the finest resolution imagery that computer hardware enables, while applying minimal point cloud filtering. The extended processing time and data storage demands of high-resolution imagery must be balanced against small reductions in tree detection performance when down-scaling image resolution to allow the processing of greater data extents. Full article

This study deals with the effect of heat treatment on Pinus oocarpa specimens from forest plantations in Colombia. The effects of two heat treatments at 170 and 190 °C for 2.5 h in saturated vapor were evaluated based on the color, dimensional stability, air-dry and basic densities, modulus of elasticity (MOE), and modulus of rupture (MOR) in static bending of samples. The evaluations were carried out following the Colombian Technical Standards NTC 290 and 663, and the color changes resulting from heat treatments were monitored using the CIE-Lab, as well as other standards from the literature. The results show that there was 2.4% and 3.3% mass loss of wood modified at 170 and 190 °C, respectively. The air-dry and basic densities were higher in 170 °C treatment than after 190 °C treatment, and the thermal modifications applied increased the dimensional stability of the treated wood. After treatment at 170 and 190 °C, the lightness to darkness (L*) was reduced by 10% and 22%; the a* coordinate increased by 11% and 26%, causing redness in the treated wood; the b* coordinate increased by 14% and 17%; and the values of the wood color saturation (c*) increased by 14% and 18%, respectively. The general color change (ΔE*) increased gradually with the increase in the treatment temperature, resulting in a high color change to a very different color. The bending strength of thermally modified wood was improved and significantly increased to values higher than those of unmodified Pinus oocarpa wood. The high air-dry and basic densities, improved dimensional stability and resistance to bending, and attractive appearance of the treated wood indicate that thermal modification is a promising alternative for the transformation of Pinus oocarpa wood into a raw material with a high added value. Full article

Models of forest growth and yield (G&Y) are a key component in long-term strategic forest management plans. Models leveraging the industry-standard “empirical” approach to G&Y are frequently underpinned by an assumption of historical consistency in climatic growing conditions. This assumption is problematic as forest managers look to obtain reliable growth predictions under the changing climate of the 21st century. Consequently, there is a pressing need for G&Y modelling approaches that can be more robustly applied under the influence of climate change. In this study we utilized an established forest gap model (JABOWA-3) to simulate G&Y between 2020 and 2100 under Representative Concentration Pathways (RCP) 2.6, 4.5, and 8.5 in the Canadian province of Newfoundland and Labrador (NL). Simulations were completed using the province’s permanent sample plot data and surface-fitted climatic datasets. Through model validation, we found simulated basal area (BA) aligned with observed BA for the major conifer species components of NL’s forests, including black spruce [Picea mariana (Mill.) Britton et al.] and balsam fir [Abies balsamea (L.) Mill]. Model validation was not as robust for the less abundant species components of NL (e.g., Acer rubrum L. 1753, Populus tremuloides Michx., and Picea glauca (Moench) Voss). Our simulations generally indicate that projected climatic changes may modestly increase black spruce and balsam fir productivity in the more northerly growing environments within NL. In contrast, we found productivity of these same species to only be maintained, and in some instances even decline, toward NL’s southerly extents. These generalizations are moderated by species, RCP, and geographic parameters. Growth modifiers were also prepared to render empirical G&Y projections more robust for use under periods of climate change. Full article

The charcoal disease agents, Biscogniauxia mediterranea and Obolarina persica are two latent, ascomycetous oak pathogens in the Middle Eastern Zagros forests, where they have devastating effects, particularly during drought. Under greenhouse conditions, we investigated the effects of the two charcoal disease agents individually and in combination with drought on survival, growth, foliar gas-exchange, pigment content, oxidative stress and the antioxidant response of Quercus infectoria and Q. libani, two of the dominant tree species in this region. Commonly, the strongest negative effects emerged in the drought–pathogen interaction treatments. Q. infectoria showed less severe lesions, higher survival, more growth, and less leaf loss than Q. libani under combined biotic and abiotic stress. In both oak species, the combination of pathogen infection and drought resulted in more than 50% reduction in foliar gas-exchange parameters with partial recovery over time in Q. infectoria suggesting a superior defense system. Indeed, enhanced foliar anthocyanin, total soluble protein and glutathione concentrations imply an upregulation of the antioxidant defense system in Q. infectoria under stress while none of these parameters showed a significant treatment response in Q. libani. Consequently, Q. infectoria foliage showed no significant increase in superoxide, lower lipoxygenase activity, and less electrolyte leakage compared to the highly elevated levels seen in Q. libani indicating oxidative damage. Our findings indicate greater drought tolerance and pathogen resilience in Q. infectoria compared to Q. libani. Under future climate scenarios, we therefore expect changes in forest community structure driven by a decline in Q. libani and closely associated organisms. Full article

The bottom-up effects of vegetation have been documented to be strong drivers of the soil food web structure and functioning in temperate forests. However, how the forest type affects the stability of the soil food web is not well known. In the Ziwuling forest region of the Loess Plateau, we selected three typical forests, Pinus tabuliformis Carrière (PT), Betula platyphylla Sukaczev (BP), and Quercus liaotungensis Koidz. (QL), to investigate the soil nematode community characteristics in the dry (April) and rainy (August) season, and analyzed their relationships with the soil properties. The results showed that the characteristics of the soil nematode communities and their seasonal variations differed markedly among the forest types. Compared to P. tabuliformis (PT), the B. platyphylla (BP) and Q. liaotungensis (QL) forests had higher plant diversity and more easily decomposed litters, which were more effective for improving the soil resource availability, thus, leading to more beneficial effects on the soil nematode community. In both the dry and rainy season, the soil nematode abundance was the highest in the BP forest. The Shannon–Wiener diversity index (H’), Pielou’s evenness index (J’), and nematode channel ratio index (NCR) were higher, while the Simpson dominance index (λ) and plant parasite index (PPI) were lower, in the BP and QL forests compared with in the PT forest. From the dry to rainy season, the total nematode abundance and the abundance of fungivores, bacterivores, and omnivore-predators, significantly increased in the QL and PT forests, and the values of the Wasilewska index (WI), maturity index (MI), H’, J’, λ, and NCR showed the most significant seasonal variability in the PT forest, which were mainly driven by changes in the soil labile C and N and the moisture content between the two seasons. Generally, the seasonal stability of the soil nematode communities was the highest in the BP forest and the poorest in the PT forest, probably due to variations in the plant diversity. Our results suggest the importance of tree species and diversity as bottom-up regulating factors of the soil food web structure, function, and seasonal stability, which has important implications for sustainable forest management in the Loess Plateau and other temperate regions. Full article

Forest landscape restoration is a widely accepted approach to sustainable forest management. In addition to revitalizing degraded sites, forest landscape restoration can increase the supply of sustainable timber and thereby reduce logging in natural forests. The current study presents a spatial land use optimization model and utilizes a linear programming algorithm that integrates timber production and timber processing chains to meet timber demand trade-offs and timber supply. The objective is to maximize yield and profit from forest plantations under volatile timber demands. The model was parameterized for a case study in Thai Nguyen Province, Vietnam, where most forest plantations grow Acacia mangium (A. mangium). Data were obtained from field surveys on tree growth, as well as from questionnaires to collect social-economic information and determine the timber demand of local wood processing mills. The integration of land use and wood utilization approaches reduces the amount of land needed to maintain a sustainable timber supply and simultaneously leads to higher yields and profits from forest plantations. This forest management solution combines economic and timber yield aspects and promotes measures focused on economic sustainability and land resource efficiency. Full article

Meeting the reporting requirements of the Kyoto Protocol has focused attention on the potential of forests in sustainably sequestering carbon (C) to mitigate the effects of rising levels of atmospheric CO₂. Much uncertainty remains concerning the ultimate effect of management on such sequestration effects. The management of woody debris (WD) and other deadwood stocks is an example of a management intervention with the scope of affecting the source-sink dynamics of forest C. Windrowing is the most commonly employed approach to the management of post-harvest WD. This study investigated the quantities of windrowed deadwood C across a chronosequence of reforested commercial Sitka spruce stands in Ireland and how its decomposition rate affected its contribution to forest C sequestration. The C stocks in windrowed WD ranged from 25 to 8 t C ha⁻¹ at the 4- and 16-year-old stands, respectively. Losses due to the decomposition of these stocks ranged from 5.15 t C ha⁻¹ yr⁻¹ at the youngest site (4 years old) to 0.68 t C ha⁻¹ yr⁻¹ at the oldest site (16 years old). Using a visual decay-class categorization of WD components and an assessment of wood density, decay rate constants were estimated for logs, branches, and stumps (the main WD constituents of windrows) as 0.037, 0.038, and 0.044, respectively. These results, derived from stand stock evaluations, were placed into context with data previously published from the same chronosequence that characterized the day-to-day fluxes to or from this pool. This comparison indicated that though only a very small quantity of C was lost in dissolved leachate form, the most significant pathway for loss was respiratory and ranged from 16 to 8 t C ha⁻¹ yr⁻¹ at the 9- and 16-year-old sites. These estimates were many times greater in extent than estimates made using a density-loss approach, the difference indicating that fragmentation and weathering play a large role in woody decomposition in intensively managed forests. Full article

A persistent problem in surface flux research is that turbulent fluxes observed by eddy covariance methods tend to be lower than the available energy. Using 7 years of eddy covariance flux observations in the Ebinur Lake National Wetland Nature Reserve (ELNWNR) in Xinjiang, Northwest China, this study analyzes the surface–atmosphere energy transfer characteristics at the station to explore variation characteristics of the energy flux and the energy balance closure (EBC), and the factors that influence EBC. The results show that: (1) diurnal and seasonal variations are observed in turbulent flux, available energy, and the partitioning of sensible and latent fluxes affected by environmental factors; (2) the degree of EBC varies significantly diurnally and seasonally, with EBC during the growing season significantly higher than during the dormant season; (3) due to the surface heterogeneity, EBC exhibits significant variations with wind direction that differ between the growing and dormant seasons; (4) environmental factors (e.g., vapor pressure deficit and air temperature) are important in limiting near-surface EBC, but they play a secondary role compared with the state of atmospheric motion. This study provides a basis for accurately assessing the material and energy exchanges between the desert Tugai forest ecosystem and the atmosphere. Full article

In the wood industry, laser technologies are commonly applied for the sawing, engraving, or perforation of solid wood and wood composites, but less knowledge exists about their effect on the joining and painting of wood materials with synthetic polymer adhesives and coatings. In this work, a CO₂ laser with irradiation doses from 2.1 to 18.8 J·cm⁻² was used for the modification of European beech (Fagus sylvatica L.) and Norway spruce (Picea abies /L./ Karst) wood surfaces—either in the native state or after covering them with a layer of polyvinyl acetate (PVAc) or polyurethane (PUR) polymer. The adhesion strength of the phase interface “synthetic polymer—wood”, evaluated by the standard EN ISO 4624, decreased significantly and proportionately in all the laser modification modes, with higher irradiation doses leading to a more apparent degradation and carbonization of the wood adherent or the synthetic polymer layer. The mold resistance of the polymers, evaluated by the standard EN 15457, increased significantly for the less mold-resistant PVAc polymer after its irradiation on the wood adherent. However, the more mold-resistant PUR polymer was able to better resist the microscopic fungi Aspergillus niger Tiegh. and Penicillium purpurogenum Stoll. when irradiation doses of higher intensity acted firstly on the wood adherent. Full article

Vegetation coverage is very important in terrestrial ecosystems and climate systems. However, the observational record of the normalized difference vegetation index (NDVI), which started in the 1980s when satellites became widely used, is too short to investigate the history of variation in vegetation coverage beyond the modern observation period. Here, we present a 189 y vegetation coverage series based on a total of 349 Mongolian pine (Pinus sylvestris var. mongolica Litv) cores from seven locations from the central–western Da Hinggan Mountains (CW–DHM), northeastern China. We found a significant relationship between tree-ring width and the regional cumulative normalized difference vegetation index (CNDVI). The correlation between the ring-width chronology and the regional June–July CNDVI (CNDVI_JJ) was significant, with r = 0.68 (n = 32, p < 0.001) and an explained variance of 45.8% (44.0% after the adjustment for the loss of the degree of freedom). On this basis, we designed a transfer function to reconstruct the CNDVI_JJ for the CW–DHM region from 1825 to 2013 CE (Common Era). During the last 189 years, there were 28 years with high CNDVI_JJ values, and another 28 years with low values. We also observed CNDVI_JJ fluctuations at the inter-annual and decadal time scales, including eight low value periods and nine high value periods. Based on our analysis, the variation in CNDVI is associated with climatic factors, such as temperature, precipitation and the Palmer Drought Severity Index (PDSI), which combines both temperature and precipitation. From 1950 to 2002 CE, the CNDVI showed a noticeable decreasing trend in the CW–DHM region, whereas after 2003 CE, the CNDVI exhibited an apparent increase, which has also been observed in southern Central Siberia, eastern Mongolia and northeastern and eastern China, indicating that the CNDVI change in the CW–DHM is related to climate change in the local region and in some parts of Asia. Full article

The development of mobile optoelectronic graders for separating viable seeds by spectrometric properties with high detection accuracy represents a very relevant direction of development for seed handling operations. Here, the main parameters of the radiation source and receiver for detecting a single seed in the diagnostic system of a mobile grader were modeled based on the principles of technical optics using Scots pine (Pinus sylvestris L.) seeds as a case study. Among the pine seeds in the seed batch, there are fossilized and empty seeds that are exactly the same in geometric and gravitational parameters as live seeds. For their separation from the seed batch, data from spectrometric studies in the near-infrared (980 nm) region can be used. To substantiate the parameters of the light source, a geometric optical model of optical beam formation was considered, while the energy model of optical beam formation was considered to substantiate the parameters of the light detector. The results of this study show that the signal value depended on the orientation of a single seed relative to the recording window. The beam angle from the radiation source should be within 45 degrees. The difference between the optical streams should be 50 microns, which made it possible to clearly detect the signal at a standard noise level of 15 microns and signal-to-noise detection accuracy ratio of 56.3 dB. This study expands theoretical knowledge in the field of the spectrometric properties of a single seed, considering the cases of its orientation relative to the optical beam, which affected the effective area of detection of the seed. The obtained data on the location of the main elements of the diagnostic system will speed up the design of mobile optoelectronic graders, and the development of a contemporary protocol for improving Scots pine seed quality. Full article

Soil respiration represents the second largest carbon flux, next to photosynthesis of the terrestrial biosphere, and thus plays a dual role in regional and global carbon cycles. However, soil respiration in Asian monsoon forests with high rainfall has rarely been studied. In this study, we continuously measured soil respiration using a 12-channel automated chamber system in a 61-year-old Japanese cedar forest in central Taiwan with annual rainfall greater than 2500 mm. A 4-year (2011–2014) continuous half-hourly dataset was used to quantify the influences of soil temperature and moisture, especially rainfall events, on both total soil respiration (Rs) and heterotrophic respiration (Rh). The annual mean Rs was approximately 10.8 t C ha⁻¹ (ranging from 10.7 to 10.9) t C ha⁻¹, with Rh contributing approximately 74.6% (ranging from 71.7% to 80.2%). Large seasonal variations in both Rs and Rh were primarily controlled by soil temperature. Over 45.8% of total annual rainfall amounts were provided by strong rainfall events (over 50 mm), and over 40% of rainfall events occurred during summers between 2012 and 2014. These strong rainfall events caused rainwater to enter soil pores and cover the soil surface, which resulted in limited soil microorganism activity and, consequently, restricted CO₂ production. The mean Q₁₀ values were 2.38 (ranging from 1.77 to 2.65) and 2.02 (ranging from 1.71 to 2.34) for Rs and Rh, respectively. The Q₁₀ values in this study, which were lower than in global forest ecosystems, may imply that the interannual Rs values observed in this study that were caused by high rainfall were less temperature-dependent than the Rs levels in global forest ecosystems. Both Rs and Rh were negatively correlated with soil moisture, which indicated that the soil moisture levels in the studied forest were usually under saturated conditions. These results also provide the lack of data for respiration in the Asian monsoon region under high-rainfall conditions. Full article

The influence that parameters crookedness and taper have on the stack volume was analyzed by using a 3D-simulation model in this study. To do so, log length, diameters at the midpoint and both ends, crookedness, bark thickness, taper and ovality were measured in 1000 logs of Scots pine. From this database, several data sets with different proportions of crooked and tapered logs in stack as well as with different degrees of taper and crookedness were created and taken as basis to simulate the stacks and carry out the analysis. The results show how the variation of these parameters influences the stack volume and provide their volume variation grades. These rates of variation were compared with measurement guidelines of some countries and previous research works. In conclusion, the parameters crookedness and taper influence the stack volume to a considerable extent. Specifically, the stack volume is increased as the crookedness degree or the proportion of crooked logs increases. In contrast, the stack volume is reduced as the taper degree or the proportion of tapered logs increases. Furthermore, the results demonstrate the capability of this simulation model to provide accurate results which can serve as a basis for future studies. Full article

Disturbances play an essential role in the shaping of temporal and spatial heterogeneity in natural community structures. The aim of this study was to provide an assessment of the deadwood influence on the chemical and biochemical properties of soils in a landslide area. The samples used to determine soil properties were collected from the entire landslide area, with locations distributed on a regular grid (50 × 50 m). The soil samples were collected from directly under the logs, and background soil samples were taken 1 m from the deadwood logs. The effect of the deadwood decomposition process was visible in the total organic carbon (C) and nitrogen (N) content and microbial activity of the soil. An increase in the enzyme activity and microbial biomass of the soil from directly beneath the deadwood was noted. In this study, it was found that a greater stock of deadwood was present in the accumulation zone, which resulted in a stronger effect of the released components on the soil cover. In order to restore landslide soils, microbial activity can be effectively stimulated by leaving deadwood on the landslide surface. Full article

The soil quality index (SQI) is based on several key indicators and is used to assess soil quality. More than 250,000 ha of walnut saplings (Juglans regia L.) were planted in previous cropland areas in the Sichuan Basin, China, using a range of soil cover types that may affect soil quality with effects that are unclear. We investigated the effects of white film (WF), black film (BF), shade netting (SN), and maize straw (MS) soil cover types and an uncovered control type (CK) on soil chemical and biological indicators and the SQI in the 0–15 cm soil layer in a young walnut forest in the Sichuan Basin over a 27-month study period. The results showed that all soil cover types increased the soil organic matter (SOM), total potassium (TK), and available potassium (AK) concentrations (p < 0.05), whereas the total nitrogen (TN) and available nitrogen (AN) concentrations were greater only in soils covered by MS than in CK (p < 0.05). The available phosphorus concentrations were 64.1 and 193.2% greater in soils covered by BF and MS treatments, respectively, than in the CK (p < 0.05). The numbers of soil faunal groups (N) were 45.7, 36.4, 37.2, and 101.5% higher in WF, BF, SN, and MS, respectively, than in CK (p < 0.05); the individual numbers (S) were 92.3, 36.2, 100.8, and 154.5% greater in WF, BF, SN, and MS, respectively, than in CK (p < 0.05). The microbial biomass carbon (MBC) was 15.5, 32.3, 45.0, and 77.1% greater in WF, BF, SN, and MS than in CK, respectively (p < 0.05). Redundancy discriminant analysis revealed strong positive interactions between biological indicators (MBC, N, and S) and SOM, AN, and AK concentrations. SOM, TN, AK, S, and MBC were the minimum required variables for the effective assessment of the SQI. All four soil cover types led to an improved SQI (p < 0.05), and MS had the greatest effect on SOM, TN, AN, AP, N, S, MBC, and SQI (p < 0.05). In conclusion, all four soil cover types increased the SOM levels, TK, AK, and MBC concentrations, soil faunal diversity, and SQI. The MS treatment was the most cost-effective and efficient measure to improve soil fertility, ecological function, and overall soil quality in the studied walnut forest. Full article

Broadleaf deciduous forests (BDFs) or dry dipterocarp forests play an important role in biodiversity conservation in tropical regions. Observations and classification of forest phenology provide valuable inputs for ecosystem models regarding its responses to climate change to assist forest management. Remotely sensed observations are often used to derive the parameters corresponding to seasonal vegetation dynamics. Data acquired from the Sentinel-1A satellite holds a great potential to improve forest type classification at a medium-large scale. This article presents an integrated object-based classification method by using Sentinel-1A and Landsat 8 OLI data acquired during different phenological periods (rainy and dry seasons). The deciduous forest and nondeciduous forest areas are classified by using NDVI (normalized difference vegetation index) from Landsat 8 cloud-free composite images taken during dry (from February to April) and rainy (from June to October) seasons. Shorea siamensis Miq. (S. siamensis), Shorea obtusa Wall. ex Blume (S. obtusa), and Dipterocarpus tuberculatus Roxb. (D. tuberculatus) in the deciduous forest area are classified based on the correlation between phenology of BDFs in Yok Don National Park and backscatter values of time-series Sentinel-1A imagery in deciduous forest areas. One hundred and five plots were selected during the field survey in the study area, consisting of dominant deciduous species, tree height, and canopy diameter. Thirty-nine plots were used for training to decide the broadleaf deciduous forest areas of the classified BDFs by the proposed method, and the other sixty-six plots were used for validation. Our proposed approach used the changes of backscatter in multitemporal SAR images to implement BDF classification mapping with acceptable accuracy. The overall accuracy of classification is about 79%, with a kappa coefficient of 0.7. Accurate classification and mapping of the BDFs using the proposed method can help authorities implement forest management in the future. Full article

In this follow-on study on aboveground biomass of shrubs and short-stature trees, we provide plant component aboveground biomass (herein ‘AGB’) as well as plant component AGB allometric models for five common boreal shrub and four common boreal short-stature tree genera/species. The analyzed plant components consist of stem, branch, and leaf organs. We found similar ratios of component biomass to total AGB for stems, branches, and leaves amongst shrubs and deciduous tree genera/species across the southern Northwest Territories, while the evergreen Picea genus differed in the biomass allocation to aboveground plant organs compared to the deciduous genera/species. Shrub component AGB allometric models were derived using the three-dimensional variable volume as predictor, determined as the sum of line-intercept cover, upper foliage width, and maximum height above ground. Tree component AGB was modeled using the cross-sectional area of the stem diameter as predictor variable, measured at 0.30 m along the stem length. For shrub component AGB, we achieved better model fits for stem biomass (60.33 g ≤ RMSE ≤ 163.59 g; 0.651 ≤ R² ≤ 0.885) compared to leaf biomass (12.62 g ≤ RMSE ≤ 35.04 g; 0.380 ≤ R² ≤ 0.735), as has been reported by others. For short-stature trees, leaf biomass predictions resulted in similar model fits (18.21 g ≤ RMSE ≤ 70.0 g; 0.702 ≤ R² ≤ 0.882) compared to branch biomass (6.88 g ≤ RMSE ≤ 45.08 g; 0.736 ≤ R² ≤ 0.923) and only slightly better model fits for stem biomass (30.87 g ≤ RMSE ≤ 11.72 g; 0.887 ≤ R² ≤ 0.960), which suggests that leaf AGB of short-stature trees (<4.5 m) can be more accurately predicted using cross-sectional area as opposed to diameter at breast height for tall-stature trees. Our multi-species shrub and short-stature tree allometric models showed promising results for predicting plant component AGB, which can be utilized for remote sensing applications where plant functional types cannot always be distinguished. This study provides critical information on plant AGB allocation as well as component AGB modeling, required for understanding boreal AGB and aboveground carbon pools within the dynamic and rapidly changing Taiga Plains and Taiga Shield ecozones. In addition, the structural information and component AGB equations are important for integrating shrubs and short-stature tree AGB into carbon accounting strategies in order to improve our understanding of the rapidly changing boreal ecosystem function. Full article

Cropping among trees with perennial legumes is one option for increasing agro-ecosystem services, such as improving the nitrogen supply and increasing soil protection by herbaceous vegetation. Moreover, cropping under the canopy of olive trees should diversify the farm production, compared to the traditional fallow management. Among perennial legumes, alfalfa (Medicago sativa L.) produces abundant biomass under Mediterranean rainfed condition. Based on this, a two-year field experiment was implemented in southern Tuscany in a rainfed olive orchard to test the competition for light effects on alfalfa biomass production and nutritive value. Light availability under the tree canopy was measured by hemispherical photos. In both years, the alfalfa yield of under-canopy varied according to the tree presence. A significant relationship between biomass production and light availability was recorded. The nutritive value of under-canopy alfalfa was similar to that of the open-grown alfalfa. However, same significant differences did however occur, between shaded and sole crop. When differences were found, under-canopy herbage was characterised by a higher content of crude protein and a lower content of fibre with respect to open-grown. In a hilly silvoarable olive orchard, alfalfa biomass accumulation was reduced mainly due to scarce light availability, therefore tree management such as pruning and plantation layout can enhance the herbage productivity. Studying shade tolerant forage legumes in order to enhance the yield and nutritive value of herbage production in rainfed agroforestry systems is essential. Full article

This paper examines the strength of wood adhesive bonds at high temperatures. The goal of this research is to better understand the conditions of heat delamination in cross laminated timber (CLT) that is exposed to fire. Heat delamination in CLT occurs when one lamination detaches from the composite panel before the char front reaches the bondline. Timber that falls from the panel, as a result of delamination, contributes additional fuel to the fire, which can cause fire regrowth, while the loss of a lamination causes a sudden loss in strength. Currently, to demonstrate that an adhesive does not delaminate, it must pass a full scale (6 m by 3 m) compartment fire test as prescribed in the PRG-320 product standard. In this work, we scaled down the mechanical loads and temperatures to 300 mm lap shear specimens. Seven different adhesives were tested and compared against solid wood controls with the same geometry as the lap shear specimens. Quasi-static tests were run where the specimens were loaded to failure at 25 °C and 260 °C, when the samples were at thermal equilibrium. Additionally, creep tests were performed where the load and temperature ramp was matched to the adhesive bondline temperatures measured in the large scale PRG-320 tests. With the exception of some of the polyurethane formulations, all adhesives passed the scaled-down creep test that resembles the PRG-320 standard. Of the polyurethane adhesives tested, only one formulation remained intact for the duration of the test. These results can be used to help better predict which adhesives may pass the PRG-320 test prior to full scale testing. Full article

Studies of biomass and carbon dynamics and community composition change after forest wind disturbance have predominantly examined trends after low and intermediate severity events, while studies after very severe wind disturbance have been few. This study documents trends in aboveground biomass and carbon across 10 years of forest recovery after severe wind disturbance. In July 1989, a tornado struck mature Tsugacanadensis-Pinusstrobus forest in northwest Connecticut, USA, causing damage across roughly 8 ha. Canopy tree damage and regeneration were surveyed in 1991 and 1999. Two primary hypotheses were tested, both of which derive from regeneration being primarily via the release of suppressed saplings rather than new seedling establishment: (1) Biomass and carbon accumulation will be faster than accumulation reported from a similar forest that experienced similar severity of wind disturbance; and (2) The stand will experience very little change in species composition or diversity. Estimated immediate post-disturbance (1989) aboveground live-tree carbon was 20.7 ± 43.9 Mg ha⁻¹ (9.9% of pre-disturbance) Ten years after the disturbance, carbon in aboveground live tree biomass increased to 37.1 ± 47.9 Mg ha⁻¹; thus for the first decade, annual accumulation averaged 1.6 Mg ha⁻¹ of carbon; this was significantly faster than the rate reported in a similar forest that experienced similar severity of wind disturbance. The species diversity of woody stems ten years after the disturbance was significantly higher (nonoverlapping confidence intervals of rarefaction curves) than pre-disturbance canopy trees. Thus, hypothesis 1 was confirmed while hypothesis 2 was rejected. This study augments the limited number of longer-term empirical studies that report biomass and carbon accumulation rates after wind disturbance, and can therefore serve as a benchmark for mechanistic and modeling research. Full article

Genetic variation and phenotypic stability in Norway spruce were studied based on provenances, families, and clones planted in trials at 12 sites in four Nordic countries. The families were generated in a factorial cross between 10 parents of Norwegian origin and 10 parents of Eastern European origin, and the clones were propagated from seedlings within 20 of the same families. Traits analyzed were survival, proportion of trees with stem defects, and tree heights. Stability was analyzed by regression analyses with the genetic entries’ annual shoot increment as the dependent variable and the total site mean as an environmental index. Information about growth and phenology traits were available from short-term tests. For tree heights, significant variance components were present both among female and male parents, but not for their interactions, indicating that non-additive genetic effects are small. Genotype × environment interactions were significant at all three genetic levels, but their variance components had considerably lower values than the variance components estimated for the effects of families and clones. For the set of families of Norwegian origin, strong relationships were observed between the timing of annual shot elongation, mortality, and height growth. Large variation was found at all three genetic levels for phenotypic stability measured by regression coefficients. A positive relationship was present between the regression coefficient and the timing of annual shoot growth for families, indicating that later flushing families responded more to a high site index. The regression coefficient can be a useful supplement to the breeding value when selecting for superior and stable genotypes. Full article

Disease outbreaks caused by introduced Phytophthora species have been increasing in British forests and woodlands in recent years. A better knowledge of the Phytophthora communities already present in the UK is of great importance when developing management and mitigation strategies for these diseases. To do this, soils were sampled in “disturbed” sites, meaning sites frequently visited by the public, with recent and new plantings or soil disturbances versus more “natural” forest and woodland sites with little disturbance or management. Phytophthora diversity was assessed using high-throughput Illumina sequencing targeting the widely accepted barcoding Internal Transcribed Spacer 1 (ITS1) region of rRNA and comparing it with the mitochondrial cytochrome c oxidase I (COI) gene. Isolation of Phytophthora was run in parallel. Nothophytophthora spp. and Phytophthora spp. were detected in 79 and 41 of the 132 locations of the 14 studied sites when using ITS or COI, respectively. A total of 20 Phytophthora amplicon sequence variants (ASVs) were assigned to known Phytophthora species from eight clades (1a, 2, 2b, 3a, 5, 6b, 7a, 8b, 8c, 8d, 10a, and 10b) and 12 ASVs from six clades (1a, 2c, 3a, 3b, 6b, 7a, 8b, 8c, and 8d) when using ITS or COI, respectively. Only at two locations were the results in agreement for ITS, COI, and isolation. Additionally, 21 and 17 unknown Phytophthora phylotypes were detected using the ITS and COI, respectively. Several Phytophthora spp. within clades 7 and 8, including very important forest pathogens such as P. austrocedri and P. ramorum, were identified and found more frequently at “disturbed” sites. Additionally, eight ASVs identified as Nothophytophthora spp. were detected representing the first report of species within this new genus in Britain. Only three species not known to be present in Britain (P. castaneae, P. capsici, and P. fallax) were detected with the ITS primers and not with COI. To confirm the presence of these or any potential new Phytophthora species, sites should be re-sampled for confirmation. Additionally, there is a need to confirm if these species are a threat to British trees and try to establish any eradication measures required to mitigate Phytophthora spread in Britain. Full article