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Micromachines 2013, 4(4), 402-413; doi:10.3390/mi4040402

Monodisperse Water-in-Oil-in-Water (W/O/W) Double Emulsion Droplets as Uniform Compartments for High-Throughput Analysis via Flow Cytometry

1
Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
2
Department of Chemical Engineering and Biotechnology, University of Cambridge, Pembroke Street, Cambridge CB2 3RA, UK
3
Melville Laboratory for Polymer Synthesis, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK
4
Institute for Life Sciences, University of Southampton, Highfield Campus, Southampton SO17 1BJ, UK
5
Radboud University Nijmegen, Institute for Molecules and Materials, Heyendaalseweg 135, Nijmegen 6525 AJ, The Netherlands
*
Authors to whom correspondence should be addressed.
Received: 5 September 2013 / Revised: 5 November 2013 / Accepted: 19 November 2013 / Published: 3 December 2013
(This article belongs to the Collection Lab-on-a-Chip)
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Abstract

Here we report the application of monodisperse double emulsion droplets, produced in a single step within partially hydrophilic/partially hydrophobic microfluidic devices, as defined containers for quantitative flow cytometric analysis. Samples with varying fluorophore concentrations were generated, and a clear correlation between dye concentration and fluorescence signals was observed. View Full-Text
Keywords: microfluidics; double emulsion; double flow-focusing geometry; flow cytometry microfluidics; double emulsion; double flow-focusing geometry; flow cytometry
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Yan, J.; Bauer, W.-A.C.; Fischlechner, M.; Hollfelder, F.; Kaminski, C.F.; Huck, W.T.S. Monodisperse Water-in-Oil-in-Water (W/O/W) Double Emulsion Droplets as Uniform Compartments for High-Throughput Analysis via Flow Cytometry. Micromachines 2013, 4, 402-413.

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