Table of Contents
Antioxidants, Volume 7, Issue 2 (February 2018)
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Cover Story (view full-size image) Vitamin C (ascorbate) is the major water-soluble antioxidant in plasma and its oxidation to [...] Read more. Vitamin C (ascorbate) is the major water-soluble antioxidant in plasma and its oxidation to dehydroascorbic acid (DHA) has been proposed as a marker of oxidative stress in vivo. We investigated the effects of different handling and processing conditions on the stability of ascorbate and DHA in whole blood and plasma samples. We also analysed DHA concentrations in a selection of different clinical cohorts. We found that careful handling and processing of samples, combined with appropriate analysis, is crucial for accurate determination of ascorbate and DHA in clinical samples. View this paper