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Alcohol-Induced Oxidative Stress in Health and Disease and the Role of Antioxidants

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A special issue of Antioxidants (ISSN 2076-3921). This special issue belongs to the section "Health Outcomes of Antioxidants and Oxidative Stress".

Deadline for manuscript submissions: closed (15 May 2022) | Viewed by 42996

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Dr. Marco Fiore

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Institute of Biochemistry and Cell Biology (IBBC), National Research Council (CNR), Department of Sense Organs, University Sapienza of Rome, Viale del Policlinico, 155 Rome, Italy
Interests: neurobiology; endocrinology; neurotrophins; oxidative stress; cancer; toxicology; addiction; antioxidants; polyphenols; alcohol use disorders
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Dr. Mauro Ceccanti

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Department of Clinical Medicine, Viale del Policlinico, 00161 Rome, Italy
Interests: alcohol abuse and alcoholism; alcoholic liver disease; alcohol detoxification

Special Issue Information

Dear Colleagues,

Acute, chronic and prenatal alcohol consumption is associated with cognitive impairment and various forms of dementia, as well as brain damage, liver damage, cancer, stroke, and so on. The mechanism underlying the neurotoxicity of ethanol is not yet clearly known; however, it is believed that it is mainly due to the production of Reactive Oxygen Species during its metabolism and to the pro-inflammatory action (increase in TNF-α, interleukin 1 and 6 in the hippocampus), especially in children exposed to alcohol during pregnancy and or lactation. Several studies have shown that alcohol is able to alter the production of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF). These neurotrophins play a crucial role in the survival, growth, and differentiation of neuronal cells and are involved in cognitive, learning, and memory processes. In alcohol abuse, many therapeutic interventions have been proposed based on various classes of nutraceuticals / phytochemicals with antioxidant functions. The use of therapies to reduce oxidative stress could contain the damage caused by alcohol. Polyphenols are a large and heterogeneous group of phytochemicals naturally produced as secondary metabolites of plants. Recent studies have shown that the administration of a solution enriched in olive polyphenols or resveratrol may prevent the oxidative damage induced by ethanol. However, the literature supporting the observation that dietary antioxidant supplementation can improve the cognitive decline that occurs with age is not entirely clear.

Dr. Marco Fiore
Prof. Dr. Mauro Ceccanti
Guest Editors

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Published Papers (8 papers)

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Research

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17 pages, 3647 KiB

Open AccessArticle

Blocking of P2X7r Reduces Mitochondrial Stress Induced by Alcohol and Electronic Cigarette Exposure in Brain Microvascular Endothelial Cells

by Naveen Mekala, Nishi Gheewala, Slava Rom, Uma Sriram and Yuri Persidsky

Antioxidants 2022, 11(7), 1328; https://doi.org/10.3390/antiox11071328 - 6 Jul 2022

Cited by 7 | Viewed by 2442

Abstract

Studies in both humans and animal models demonstrated that chronic alcohol/e-cigarette (e-Cig) exposure affects mitochondrial function and impairs barrier function in brain microvascular endothelial cells (BMVECs). Identification of the signaling pathways by which chronic alcohol/e-Cig exposure induces mitochondrial damage in BMVEC is vital for protection of the blood–brain barrier (BBB). To address the issue, we treated human BMVEC [hBMVECs (D3 cell-line)] with ethanol (ETH) [100 mM], acetaldehyde (ALD) [100 μM], or e-cigarette (e-Cig) [35 ng/mL of 1.8% or 0% nicotine] conditioned medium and showed reduced mitochondrial oxidative phosphorylation (OXPHOS) measured by a Seahorse analyzer. Seahorse data were further complemented with the expression of mitochondrial OXPHOS proteins detected by Western blots. We also observed cytosolic escape of ATP and its extracellular release due to the disruption of mitochondrial membrane potential caused by ETH, ALD, or 1.8% e-Cig exposure. Moreover ETH, ALD, or 1.8% e-Cig treatment resulted in elevated purinergic P2X7r and TRPV1 channel gene expression, measured using qPCR. We also demonstrated the protective role of P2X7r antagonist A804598 (10 μM) in restoring mitochondrial oxidative phosphorylation levels and preventing extracellular ATP release. In a BBB functional assay using trans-endothelial electrical resistance, we showed that blocking the P2X7r channel enhanced barrier function. In summary, we identified the potential common pathways of mitochondrial injury caused by ETH, ALD, and 1.8% e-Cig which allow new protective interventions. We are further investigating the potential link between P2X7 regulatory pathways and mitochondrial health. Full article

(This article belongs to the Special Issue Alcohol-Induced Oxidative Stress in Health and Disease and the Role of Antioxidants)

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22 pages, 4235 KiB

Open AccessArticle

Folic Acid Homeostasis and Its Pathways Related to Hepatic Oxidation in Adolescent Rats Exposed to Binge Drinking

by María del Carmen Gallego-Lopez, María Luisa Ojeda, Inés Romero-Herrera, Fátima Nogales and Olimpia Carreras

Antioxidants 2022, 11(2), 362; https://doi.org/10.3390/antiox11020362 - 11 Feb 2022

Cited by 8 | Viewed by 2610

Abstract

Chronic ethanol consumption and liver disease are intimately related to folic acid (FA) homeostasis. Despite the fact that FA decreases lipid oxidation, its mechanisms are not yet well elucidated. Lately, adolescents have been practising binge drinking (BD), consisting of the intake of a high amount of alcohol in a short time; this is a particularly pro-oxidant form of consumption. The aim of this study is to examine, for the first time, FA homeostasis in BD adolescent rats and its antioxidant properties in the liver. We used adolescent rats, including control rats and rats exposed to an intermittent intraperitoneal BD model, supplemented with or without FA. Renal FA reabsorption and renal FA deposits were increased in BD rats; hepatic deposits were decreased, and heart and serum levels remained unaffected. This depletion in the liver was accompanied by higher transaminase levels; an imbalance in the antioxidant endogenous enzymatic system; lipid and protein oxidation; a decrease in glutathione (GSH) levels; hyper-homocysteinemia (HHcy); an increase in NADPH oxidase (NOX) 1 and NOX4 enzymes; an increase in caspase 9 and 3; and a decrease in the anti-apoptotic metallopeptidase inhibitor 1. Furthermore, BD exposure increased the expression of uncoupled endothelial nitric oxide synthase (eNOS) by increasing reactive nitrogen species generation and the nitration of tyrosine proteins. When FA was administered, hepatic FA levels returned to normal levels; transaminase and lipid and protein oxidation also decreased. Its antioxidant activity was due, in part, to the modulation of superoxide dismutase activity, GSH synthesis and NOX1, NOX4 and caspase expression. FA reduced HHcy and increased the expression of coupled eNOS by increasing tetrahydrobiopterin expression, avoiding nitrosative stress. In conclusion, FA homeostasis and its antioxidant properties are affected in BD adolescent rats, making it clear that this vitamin plays an important role in the oxidative, nitrosative and apoptotic hepatic damage generated by acute ethanol exposure. For this, FA supplementation becomes a potential BD therapy for adolescents, preventing future acute alcohol-related harms. Full article

(This article belongs to the Special Issue Alcohol-Induced Oxidative Stress in Health and Disease and the Role of Antioxidants)

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14 pages, 2621 KiB

Open AccessArticle

Downregulation of Glutathione-Mediated Detoxification Capacity by Binge Drinking Aggravates Acetaminophen-Induced Liver Injury through IRE1α ER Stress Signaling

by Sou Hyun Kim, Hun Ji Choi, Hyeji Seo, Doyoung Kwon, Jaesuk Yun and Young-Suk Jung

Antioxidants 2021, 10(12), 1949; https://doi.org/10.3390/antiox10121949 - 5 Dec 2021

Cited by 7 | Viewed by 4052

Abstract

Overdose of acetaminophen (APAP) can cause severe liver injury. Although alcohol is considered a risk factor for APAP toxicity, the mechanism underlying the interaction between alcohol and APAP remains unclear. Binge alcohol (5 g/kg every 12 h, 3 doses) reduced the concentration of cysteine and glutathione (GSH) and decreased expression of cystathionine β-synthase (CβS), cystathionine γ-lyase (CγL), and glutamate cysteine ligase catalytic subunit (GCLC) in the livers of male C57BL/6 mice. Furthermore, the levels of GSH S-transferase (GST) and GSH peroxidase (GPx) were decreased. To evaluate the effect of binge drinking on APAP-induced liver injury, 300 mg APAP was administered following alcohol binges. APAP in the binge group significantly amplified the serum ALT more than two fold and enhanced the pro-apoptotic proteins with a severe centrilobular necrosis compared to APAP alone. APAP treatment after alcohol binges caused lower levels of hepatic cysteine and GSH than APAP alone over 24 h, indicating that alcohol binges reduced GSH regenerating potential. Exposure to APAP after binge treatment significantly increased oxidative stress (lipid peroxidation) and endoplasmic reticulum (ER) stress (Grp78 and ATF6) markers at 6 h after treatment. Notably, the IRE1α/ASK1/MKK4/JNK pathway was activated, whereas CHOP expression was reduced by APAP administration in mice with pre-exposed alcohol binges compared with APAP alone. Thus, pretreatment with binge alcohol decreases GSH-mediated antioxidant capacity and contributes to augmentation of liver injury caused by subsequent APAP administration through differential ER stress signaling pathway. Full article

(This article belongs to the Special Issue Alcohol-Induced Oxidative Stress in Health and Disease and the Role of Antioxidants)

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12 pages, 462 KiB

Open AccessArticle

Ascorbic Acid Deficiency Prevalence and Associated Cognitive Impairment in Alcohol Detoxification Inpatients: A Pilot Study

by Virgile Clergue-Duval, Julien Azuar, Julien Fonsart, Clément Delage, Dorian Rollet, Jihed Amami, Alexia Frapsauce, Marie-Astrid Gautron, Eric Hispard, Frank Bellivier, Vanessa Bloch, Jean-Louis Laplanche, Frank Questel and Florence Vorspan

Antioxidants 2021, 10(12), 1892; https://doi.org/10.3390/antiox10121892 - 26 Nov 2021

Cited by 8 | Viewed by 3627

Abstract

Malnutrition has been reported in alcohol use disorder patients as having a possible influence on cognitive function. The aim of this study was to analyse the prevalence of ascorbic acid (AA) deficiency in inpatients admitted for alcohol detoxification and the associated factors, including cognitive impairment in the early period of abstinence. A retrospective chart review was conducted. The AA level was categorised into three groups: deficiency (AAD) (<2 mg/L), insufficiency (AAI) (2–5 mg/L) and normal level. The cognitive impairment was screened using the Montreal Cognitive Assessment (MoCA). Ninety-six patients were included (74 men; mean age 49.1 years (±11.5)). Twenty-seven AAD (28.1%) and twenty-two AAI (22.9%) were observed. In multivariate analysis, risk factors for AAD versus normal AA level were men (OR 17.8, 95%CI (1.63–194)), compensated cirrhosis (OR 9.35, 95%CI (1.60–54.6)) and street homelessness (OR 5.76, 95%CI (1.24–26.8) versus personal housing). The MoCA score was available for 53 patients (mean MoCA score: 25.7 (±3.3)). In multivariate analysis, the natural logarithm of AA (β = 1.18, p = 0.037) and sedative use disorder (β = −2.77, p = 0.046) were associated with the MoCA score. AAD and AAI are frequent in inpatients admitted for alcohol detoxification. A low level of AA was associated with cognitive impairment in the early period of abstinence. Full article

(This article belongs to the Special Issue Alcohol-Induced Oxidative Stress in Health and Disease and the Role of Antioxidants)

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Review

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12 pages, 860 KiB

Open AccessReview

Alcohol Withdrawal Is an Oxidative Stress Challenge for the Brain: Does It Pave the Way toward Severe Alcohol-Related Cognitive Impairment?

by Virgile Clergue-Duval, Laurent Coulbault, Frank Questel, Nicolas Cabé, Alice Laniepce, Clément Delage, Céline Boudehent, Vanessa Bloch, Shailendra Segobin, Mickael Naassila, Anne-Lise Pitel and Florence Vorspan

Antioxidants 2022, 11(10), 2078; https://doi.org/10.3390/antiox11102078 - 21 Oct 2022

Cited by 4 | Viewed by 5253

Abstract

Alcohol use is a leading cause of mortality, brain morbidity, neurological complications and minor to major neurocognitive disorders. Alcohol-related neurocognitive disorders are consecutive to the direct effect of chronic and excessive alcohol use, but not only. Indeed, patients with severe alcohol use disorders (AUD) associated with pharmacological dependence suffer from repetitive events of alcohol withdrawal (AW). If those AW are not managed by adequate medical and pharmacological treatment, they may evolve into severe AW, or be complicated by epileptic seizure or delirium tremens (DT). In addition, we suggest that AW favors the occurrence of Wernicke’s encephalopathy (WE) in patients with known or unknown thiamine depletion. We reviewed the literature on oxidative stress as a core mechanism in brain suffering linked with those conditions: AW, epileptic seizure, DT and WE. Thus, we propose perspectives to further develop research projects aiming at better identifying oxidative stress brain damage related to AW, assessing the effect of repetitive episodes of AW, and their long-term cognitive consequences. This research field should develop neuroprotective strategies during AW itself or during the periwithdrawal period. This could contribute to the prevention of severe alcohol-related brain damage and cognitive impairments. Full article

(This article belongs to the Special Issue Alcohol-Induced Oxidative Stress in Health and Disease and the Role of Antioxidants)

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26 pages, 1968 KiB

Open AccessReview

Ethanol Metabolism in the Liver, the Induction of Oxidant Stress, and the Antioxidant Defense System

by Martha Lucinda Contreras-Zentella, Daniel Villalobos-García and Rolando Hernández-Muñoz

Antioxidants 2022, 11(7), 1258; https://doi.org/10.3390/antiox11071258 - 26 Jun 2022

Cited by 45 | Viewed by 8214

Abstract

The liver metabolizes ethanol through three enzymatic pathways: alcohol dehydrogenase (ADH), cytochrome p450 (also called MEOS), and catalase. Alcohol dehydrogenase class I (ADH1) is considered the most important enzyme for the metabolism of ethanol, MEOS and catalase (CAT) are considered minor alternative pathways. However, contradicting experiments suggest that the non-ADH1 pathway may have a greater relevance for the metabolism of ethanol than previously thought. In some conditions, ethanol is predominately metabolized to acetaldehyde via cytochrome P450 family 2 (CYP2E1), which is involved in the generation of reactive oxygen species (ROS), mainly through electron leakage to oxygen to form the superoxide (O₂^•−) radical or in catalyzed lipid peroxidation. The CAT activity can also participate in the ethanol metabolism that produces ROS via ethanol directly reacting with the CAT-H₂O₂ complex, producing acetaldehyde and water and depending on the H₂O₂ availability, which is the rate-limiting component in ethanol peroxidation. We have shown that CAT actively participates in lactate-stimulated liver ethanol oxidation, where the addition of lactate generates H₂O₂, which is used by CAT to oxidize ethanol to acetaldehyde. Therefore, besides its known role as a catalytic antioxidant component, the primary role of CAT could be to function in the metabolism of xenobiotics in the liver. Full article

(This article belongs to the Special Issue Alcohol-Induced Oxidative Stress in Health and Disease and the Role of Antioxidants)

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24 pages, 1028 KiB

Open AccessReview

Alcohol and Head and Neck Cancer: Updates on the Role of Oxidative Stress, Genetic, Epigenetics, Oral Microbiota, Antioxidants, and Alkylating Agents

by Giampiero Ferraguti, Sergio Terracina, Carla Petrella, Antonio Greco, Antonio Minni, Marco Lucarelli, Enzo Agostinelli, Massimo Ralli, Marco de Vincentiis, Giammarco Raponi, Antonella Polimeni, Mauro Ceccanti, Brunella Caronti, Maria Grazia Di Certo, Christian Barbato, Alessandro Mattia, Luigi Tarani and Marco Fiore

Antioxidants 2022, 11(1), 145; https://doi.org/10.3390/antiox11010145 - 11 Jan 2022

Cited by 34 | Viewed by 4702

Abstract

Head and neck cancer (HNC) concerns more than 890,000 patients worldwide annually and is associated with the advanced stage at presentation and heavy outcomes. Alcohol drinking, together with tobacco smoking, and human papillomavirus infection are the main recognized risk factors. The tumorigenesis of HNC represents an intricate sequential process that implicates a gradual acquisition of genetic and epigenetics alterations targeting crucial pathways regulating cell growth, motility, and stromal interactions. Tumor microenvironment and growth factors also play a major role in HNC. Alcohol toxicity is caused both directly by ethanol and indirectly by its metabolic products, with the involvement of the oral microbiota and oxidative stress; alcohol might enhance the exposure of epithelial cells to carcinogens, causing epigenetic modifications, DNA damage, and inaccurate DNA repair with the formation of DNA adducts. Long-term markers of alcohol consumption, especially those detected in the hair, may provide crucial information on the real alcohol drinking of HNC patients. Strategies for prevention could include food supplements as polyphenols, and alkylating drugs as therapy that play a key role in HNC management. Indeed, polyphenols throughout their antioxidant and anti-inflammatory actions may counteract or limit the toxic effect of alcohol whereas alkylating agents inhibiting cancer cells’ growth could reduce the carcinogenic damage induced by alcohol. Despite the established association between alcohol and HNC, a concerning pattern of alcohol consumption in survivors of HNC has been shown. It is of primary importance to increase the awareness of cancer risks associated with alcohol consumption, both in oncologic patients and the general population, to provide advice for reducing HNC prevalence and complications. Full article

(This article belongs to the Special Issue Alcohol-Induced Oxidative Stress in Health and Disease and the Role of Antioxidants)

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Other

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33 pages, 1619 KiB

Open AccessSystematic Review

Alcohol-Induced Oxidative Stress and the Role of Antioxidants in Alcohol Use Disorder: A Systematic Review

by Evangelia Eirini Tsermpini, Anja Plemenitaš Ilješ and Vita Dolžan

Antioxidants 2022, 11(7), 1374; https://doi.org/10.3390/antiox11071374 - 15 Jul 2022

Cited by 48 | Viewed by 9954

Abstract

Alcohol use disorder (AUD) is a highly prevalent, comorbid, and disabling disorder. The underlying mechanism of ethanol neurotoxicity and the involvement of oxidative stress is still not fully elucidated. However, ethanol metabolism has been associated with increased oxidative stress through alcohol dehydrogenase, the microsomal ethanol oxidation system, and catalase metabolic pathways. We searched the PubMed and genome-wide association studies (GWAS) catalog databases to review the literature systematically and summarized the findings focusing on AUD and alcohol abstinence in relation to oxidative stress. In addition, we reviewed the ClinicalTrials.gov resource of the US National Library of Medicine to identify all ongoing and completed clinical trials that include therapeutic interventions based on antioxidants. The retrieved clinical and preclinical studies show that oxidative stress impacts AUD through genetics, alcohol metabolism, inflammation, and neurodegeneration. Full article

(This article belongs to the Special Issue Alcohol-Induced Oxidative Stress in Health and Disease and the Role of Antioxidants)

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