Yeast Biotechnology for Food Industrial Processes

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Food Biotechnology".

Deadline for manuscript submissions: 27 June 2024 | Viewed by 2011

Special Issue Editors


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Guest Editor
Instituto de Agroquímica y Tecnología de los Alimentos (IATA-CSIC), 46980 Paterna, Valencia, Spain
Interests: wine yeasts; fermentation; genomics; QTL; ALE; hybrids

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Guest Editor
Instituto de Agroquímica y Tecnología de los Alimentos (IATA-CSIC), 46980 Paterna, Valencia, Spain
Interests: wine yeasts; wine fermentation
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Special Issue Information

Dear Colleagues,

Wine fermentation and other industrial processes are harsh environments mainly dominated by the yeast Saccharomyces cerevisiae, which is well equipped to overcome many of these stresses. However, the limited genetic diversity of this species may not fulfill the new trends in the wine industry. In the last few years, less conventional yeasts have received a great amount of interest regarding their potential to improve some organoleptic properties, as well as to generate interspecific hybrids with S. cerevisiae. In this context, biotechnological approaches to generate tailored yeast strains better adapted to different fermentative conditions have been developed. Regulations and consumers’ perception for genetic engineering have pushed wine biotechnology towards alternative techniques, including mutagenesis, hybridization, or adaptive evolution, while benefiting from a non-GMO status. Recent advances in multiomics and NGS have also boosted yeast biotechnology. However, genome editing represents a worldwide revolution, exerting high expectations among wine biotechnologists about the decrease in the restrictions for CRISPR/Cas-derived strains in the near future.

Dr. Estéfani García-Ríos
Dr. José Manuel Guillamón
Guest Editors

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Keywords

  • wine
  • Saccharomyces cerevisiae
  • non-Saccharomyces yeasts
  • genetically modified organisms
  • gene editing
  • hybridization
  • adaptive laboratory evolution

Published Papers (2 papers)

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Research

25 pages, 2921 KiB  
Article
Screening, Identification, and Fermentation Condition Optimization of a High-Yield 3-Methylthiopropanol Yeast and Its Aroma-Producing Characteristics
by Yujiao Zhang, Qi Sun, Xiaoyan Liu, Rana Abdul Basit, Jinghao Ma, Zhilei Fu, Liujie Cheng, Guangsen Fan and Chao Teng
Foods 2024, 13(3), 418; https://doi.org/10.3390/foods13030418 - 27 Jan 2024
Cited by 1 | Viewed by 1072
Abstract
A high-yield 3-methylthiopropanol (3-Met) yeast Y1402 was obtained from sesame-flavored Daqu, and it was identified as Saccharomycopsis fibuligera. S. fibuligera Y1402 showed a broad range of growth temperatures and pH, as well as the maximum tolerance to glucose, NaCl, nicotine, and 3-Met [...] Read more.
A high-yield 3-methylthiopropanol (3-Met) yeast Y1402 was obtained from sesame-flavored Daqu, and it was identified as Saccharomycopsis fibuligera. S. fibuligera Y1402 showed a broad range of growth temperatures and pH, as well as the maximum tolerance to glucose, NaCl, nicotine, and 3-Met at 50% (w/w), 15% (w/v), 1.2 g/L, and 18 g/L, respectively. After optimization using single-factor experiments, a Plackett–Burman design, a steepest ascent test, and a Box–Behnken design, the 3-Met yield reached 4.03 g/L by S. fibuligera Y1402 under the following optimal conditions: glucose concentration of 40 g/L, yeast extract concentration of 0.63 g/L, Tween 80 concentration of 2 g/L, L-methionine concentration of 5 g/L, liquid volume of 25 mL/250 mL, initial pH of 5.3, fermentation temperature of 32 °C, inoculum size of 0.8%, shaking speed of 210 rpm, and fermentation time of 54 h. The fermentation was scaled up to a 3 L fermenter under the optimized conditions, and the yield of 3-Met reached 0.71 g/L. Additionally, an aroma analysis revealed that the flavor substances produced by S. fibuligera Y1402 in sorghum hydrolysate medium was mainly composed of compounds with floral, sweet, creamy, roasted nut, and clove-like aromas. Therefore, S. fibuligera has great potential for application in the brewing of Baijiu and other fermented foods. Full article
(This article belongs to the Special Issue Yeast Biotechnology for Food Industrial Processes)
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13 pages, 3734 KiB  
Article
Biocontrol Using Torulaspora delbrueckii in Sequential Fermentation: New Insights into Low-Sulfite Verdicchio Wines
by Laura Canonico, Alice Agarbati, Edoardo Galli, Francesca Comitini and Maurizio Ciani
Foods 2023, 12(15), 2899; https://doi.org/10.3390/foods12152899 - 30 Jul 2023
Viewed by 623
Abstract
Torulaspora delbrueckii has attracted renewed interest in recent years, for its biotechnological potential linked to its ability to enhance the flavor and aroma complexity of wine. Sequential fermentations with a selected native strain of T. delbrueckii (DiSVA 130) and low-sulfite native strain of [...] Read more.
Torulaspora delbrueckii has attracted renewed interest in recent years, for its biotechnological potential linked to its ability to enhance the flavor and aroma complexity of wine. Sequential fermentations with a selected native strain of T. delbrueckii (DiSVA 130) and low-sulfite native strain of Saccharomyces cerevisiae (DiSVA 709) were carried out to establish their contribution in biocontrol and the aroma profile. A first set of trials were conducted to evaluate the effect of the sulfur dioxide addition on pure and T. debrueckii/S. cerevisiae sequential fermentations. A second set of sequential fermentations without SO2 addition were conducted to evaluate the biocontrol and aromatic effectiveness of T. delbrueckii. Native T. delbrueckii showed a biocontrol action in the first two days of fermentation (wild yeasts reduced by c.a. 1 log at the second day). Finally, trials with the combination of both native and commercial T. delbrueckii/S. cerevisiae led to distinctive aromatic profiles of wines, with a significant enhancement in isoamyl acetate, phenyl ethyl acetate, supported by positive appreciations from the tasters, for ripe and tropical fruits, citrus, and balance. The whole results indicate that native T. delbrueckii could be a potential biocontrol tool against wild yeasts in the first phase of fermentation, contributing to improving the final wine aroma. Full article
(This article belongs to the Special Issue Yeast Biotechnology for Food Industrial Processes)
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