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Micromachines
Special Issues
Microfluidic Cell Assay Chips, Volume II
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Microfluidic Cell Assay Chips, Volume II

A special issue of Micromachines (ISSN 2072-666X). This special issue belongs to the section "B：Biology and Biomedicine".

Deadline for manuscript submissions: closed (20 June 2022) | Viewed by 3683

Special Issue Editor

Dr. Yu-Chih Chen

E-Mail Website
Guest Editor
1. UPMC Hillman Cancer Center, University of Pittsburgh, Pittsburgh, PA 15260, USA
2. Department of Computational and Systems Biology, University of Pittsburgh, Pittsburgh, PA 15260, USA
3. CMU-Pitt Ph.D. Program in Computational Biology, Pittsburgh, PA, USA
4. Department of Bioengineering, Swanson School of Engineering, University of Pittsburgh, Pittsburgh, PA 15260, USA
Interests: single-cell analysis; cancer precision medicine; microfluidics; machine learning; gene sequencing
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Microfluidic technology has emerged as a state-of-the-art approach for cell biology because of precise micro-environment manipulation, minimal reagent usage, and high potential in scaling and automation. Recently, microfluidic cell assay chips have demonstrated their capabilities of drug screening, 3D cell culture, cell migration and invasion, cell–cell interaction, single cell analysis, transcriptomic and proteomic profiling, and clinical diagnostics. Though core functions were developed as prototypes, there is a recognized need to provide low-cost and reliable manufacturing methods for dissemination of the technology. Further development in automation and system integration will be required to realize the full potential of high-throughput assays and readouts. In addition, the smart interface between microfluidics and conventional bulk machines is critical for handling small sample volumes and saving reagents. In light of these prevailing challenges, this Special Issue seeks to collect research papers, short communications, and review articles that focus on but are not limited to novel microfluidic cell assays, low-cost and reliable micromanufacturing methods, 3D-printed microfluidics, high-throughput experimentation, automation, and smart interface for microfluidic cell analysis.

Dr. Yu-Chih Chen
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Micromachines is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • microfluidic cell assays
  • lab on a chip
  • micrototal analysis systems
  • high-throughput screening
  • micro-manufacturing
  • micro-machining
  • 3D printing
  • microfluidic interface
  • microfluidic system integration
  • clinical diagnostics

Published Papers (2 papers)

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Research

13 pages, 3651 KiB  
Article
A Rapid and Simple Method for Purification of Nucleic Acids on Porous Membranes: Simulation vs. Experiment
by Angela Fonseca-Benitez, Consuelo Romero-Sánchez and Sandra Janneth Perdomo Lara
Micromachines 2022, 13(12), 2238; https://doi.org/10.3390/mi13122238 - 16 Dec 2022
Cited by 2 | Viewed by 1622
Abstract
Paper-based microfluidic systems have emerged as one of the most promising technologies for developing point-of-care diagnostic platforms (POCT) for detecting and monitoring various diseases. Saliva is a non-invasive biofluid easily collected, transported, and stored. Due to its accessibility and connection to systemic diseases, [...] Read more.
Paper-based microfluidic systems have emerged as one of the most promising technologies for developing point-of-care diagnostic platforms (POCT) for detecting and monitoring various diseases. Saliva is a non-invasive biofluid easily collected, transported, and stored. Due to its accessibility and connection to systemic diseases, saliva is one of the best candidates for medical advancement at the point of care, where people can easily monitor their health. However, saliva is a complex mixture of DNA, RNA, proteins, exosomes, and electrolytes. Thus, nucleic acid separation from the salivary components is essential for PCR applications. Paper membranes are a highly porous and foldable structure capable of transporting fluids without pumps and sophisticated systems. The current work presents an insight into simulations for nucleic acid extraction on three types of porous paper membranes for use in point-of-care devices. The flow fluid model is solved on a COMSOL Multiphysics 5.3 free version platform, and the results are compared with experimental assays. The results show that pore uniformity, wet strength, porosity, and functional groups of MF1™ and Fusion 5™ paper membranes are vital parameters affecting nucleic acid extraction and PCR amplification efficiency. Full article
(This article belongs to the Special Issue Microfluidic Cell Assay Chips, Volume II)
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11 pages, 2452 KiB  
Article
A Resistance-Based Microfluidic Chip for Deterministic Single Cell Trapping Followed by Immunofluorescence Staining
by Xiange Sun, Bowen Li, Wenman Li, Xiaodong Ren, Ning Su, Ruoxu Li, Jinmi Li and Qing Huang
Micromachines 2022, 13(8), 1272; https://doi.org/10.3390/mi13081272 - 07 Aug 2022
Cited by 4 | Viewed by 1624
Abstract
Microchips are fundamental tools for single-cell analysis. Although various microfluidic methods have been developed for single-cell trapping and analysis, most microchips cannot trap single cells deterministically for further analysis. In this paper, we describe a novel resistance-based microfluidic chip to implement deterministic single-cell [...] Read more.
Microchips are fundamental tools for single-cell analysis. Although various microfluidic methods have been developed for single-cell trapping and analysis, most microchips cannot trap single cells deterministically for further analysis. In this paper, we describe a novel resistance-based microfluidic chip to implement deterministic single-cell trapping followed by immunofluorescence staining based on the least flow resistance principle. The design of a large circular structure before the constriction and the serpentine structure of the main channel made the flow resistance of the main channel higher than that of the trapping channel. Since cells preferred to follow paths with lower flow resistance, this design directed cells into the capture sites and improved single-cell trapping efficiency. We optimized the geometric parameters using numerical simulations. Experiments using A549 and K562 cell lines demonstrated the capability of our chip with (82.7 ± 2.4)% and (84 ± 3.3)% single-cell trapping efficiency, respectively. In addition, cells were immobilized at capture sites by applying the pulling forces at the outlet, which reduced the cell movement and loss and facilitated tracking of the cell in real time during the multistep immunofluorescence staining procedure. Due to the simple operation, high-efficiency single-cell trapping and lower cell loss, the proposed chip is expected to be a potential analytical platform for single tumor cell heterogeneity studies and clinical diagnosis. Full article
(This article belongs to the Special Issue Microfluidic Cell Assay Chips, Volume II)
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