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Natural Products: Isolation, Identification and Biological Activity II

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Natural Products Chemistry".

Deadline for manuscript submissions: closed (30 April 2024) | Viewed by 2068

Special Issue Editors


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Guest Editor
Department of Inorganic Chemistry, Medical University of Lublin, Lublin, Poland
Interests: liquid chromatography; optimization of chromatographic systems for separation of ionic compounds; quantitative analysis of ionic compounds in plant extracts; biological activity of plant extracts
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
Department of Physical Chemistry, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, Poland
Interests: liquid chromatography with modern detection techniques; sample preparation; analysis of xenobiotics in various biological samples; analysis of ionic compounds in plant extracts; biological activity of plant extracts
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Natural products have been used for treatment and alleviate various diseases since early human history. One of the notable sources of natural products is medicinal plants. A number of phytochemicals such as polyphenols, carotenoids, polysaccharides, volatile oils, alkaloids and many others are responsible for their pharmacological properties. It is well established that these activities are associated with the great number of secondary metabolites produced by plants to adapt themselves to the environment. Natural products are still a beneficial source to obtain and isolate compounds with pharmacological applications or can also be used as herbal medicinal products in traditional or complementary medicine.

However, after years of research in this field and in spite of the remarkable recent advances in chemical synthesis, natural products remain the most powerful and somewhat under-investigated source of bioactive compounds. There are still many species of plants tthat have still not been screened for pharmacological application and might be a cause for a breakthrough in science. Therefore, more investigations should be done in various species of plants and other natural products. Currently, the most important directions are develop effective and selective methods for the isolation of bioactive compounds from natural products, the identification and quantification of those compounds, and in vitro and then in vivo investigations on their biological activity.

Despite significant progress in sample preparation and separation techniques, the isolation and identification of natural products from natural products and investigations on their biological activity are still are difficult and important tasks.

The papers include coverage of work on natural substances of the land and sea and of plants, microbes, and animals. Investigations on isolation, identification, structure elucidation and various biological activities are welcomed in this Special Issue.

We cordially invite researchers working in this field to contribute original research articles, short communications, and critical review articles.

Prof. Dr. Anna Petruczynik
Prof. Dr. Tomasz Tuzimski
Guest Editors

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • plant extracts
  • isolation of active compounds from natural products
  • analysis of natural products
  • identyfication and quantification of natural products components
  • determination of biological activity

Published Papers (3 papers)

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Research

11 pages, 5648 KiB  
Communication
Lycocasine A, a Lycopodium Alkaloid from Lycopodiastrum casuarinoides and Its Acid-Sensing Ion Channel 1a Inhibitory Activity
by Shuai Jiang, Wen-Yan Li, Bei-Bei Gao and Qin-Shi Zhao
Molecules 2024, 29(7), 1581; https://doi.org/10.3390/molecules29071581 - 01 Apr 2024
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Abstract
A novel Lycopodium alkaloid, lycocasine A (1), and seven known Lycopodium alkaloids (28), were isolated from Lycopodiastrum casuarinoides. Their structures were determined through NMR, HRESIMS, and X-ray diffraction analysis. Compound 1 features an unprecedented 5/6/6 tricyclic [...] Read more.
A novel Lycopodium alkaloid, lycocasine A (1), and seven known Lycopodium alkaloids (28), were isolated from Lycopodiastrum casuarinoides. Their structures were determined through NMR, HRESIMS, and X-ray diffraction analysis. Compound 1 features an unprecedented 5/6/6 tricyclic skeleton, highlighted by a 5-aza-tricyclic[6,3,1,02,6]dodecane motif. In bioactivity assays, compound 1 demonstrated weak inhibitory activity against acid-sensing ion channel 1a. Full article
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37 pages, 14298 KiB  
Article
In Vitro and In Silico of Cholinesterases Inhibition and In Vitro and In Vivo Anti-Melanoma Activity Investigations of Extracts Obtained from Selected Berberis Species
by Tomasz Tuzimski, Anna Petruczynik, Barbara Kaproń, Tomasz Plech, Anna Makuch-Kocka, Daria Janiszewska, Mateusz Sugajski, Bogusław Buszewski and Małgorzata Szultka-Młyńska
Molecules 2024, 29(5), 1048; https://doi.org/10.3390/molecules29051048 - 28 Feb 2024
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Abstract
Berberis species have a long history of use in traditional Chinese medicine, Ayurvedic medicine, and Western herbal medicine. The aim of this study was the quantification of the main isoquinoline alkaloids in extracts obtained from various Berberis species by HPLC, in vitro and [...] Read more.
Berberis species have a long history of use in traditional Chinese medicine, Ayurvedic medicine, and Western herbal medicine. The aim of this study was the quantification of the main isoquinoline alkaloids in extracts obtained from various Berberis species by HPLC, in vitro and in silico determination of anti-cholinesterase activity, and in vitro and in vivo investigations of the cytotoxic activity of the investigated plant extracts and alkaloid standards. In particular, Berberis species whose activity had not been previously investigated were selected for the study. In the most investigated Berberis extracts, a high content of berberine and palmatine was determined. Alkaloid standards and most of the investigated plant extracts exhibit significant anti-cholinesterase activity. Molecular docking results confirmed that both alkaloids are more favourable for forming complexes with acetylcholinesterase compared to butyrylcholinesterase. The kinetic results obtained by HPLC-DAD indicated that berberine noncompetitively inhibited acetylcholinesterase, while butyrylcholinesterase was inhibited in a mixed mode. In turn, palmatine exhibited a mixed inhibition of acetylcholinesterase. The cytotoxic activity of berberine and palmatine standards and plant extracts were investigated against the human melanoma cell line (A375). The highest cytotoxicity was determined for extract obtained from Berberis pruinosa cortex. The cytotoxic properties of the extract were also determined in the in vivo investigations using the Danio rerio larvae xenograft model. The obtained results confirmed a significant effect of the Berberis pruinosa cortex extract on the number of cancer cells in a living organism. Our results showed that extracts obtained from Berberis species, especially the Berberis pruinosa cortex extract, can be recommended for further in vivo experiments in order to confirm the possibility of their application in the treatment of neurodegenerative diseases and human melanoma. Full article
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13 pages, 1530 KiB  
Article
Isoprenylated Flavonoids and 2-Arylbenzofurans from the Root Bark of Morus alba L. and Their Cytotoxic Activity against HGC27 Cancer Cells
by Hangyi Pu, Dongyi Cao, Xue Zhou, Fu Li, Lun Wang and Mingkui Wang
Molecules 2024, 29(1), 30; https://doi.org/10.3390/molecules29010030 - 20 Dec 2023
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Abstract
Three new compounds (1, 11, and 12), together with 32 known ones, were isolated from the root bark of Morus alba L. using various chromatographic methods. The structures of the undescribed compounds were elucidated based on 1D, 2D NMR, [...] Read more.
Three new compounds (1, 11, and 12), together with 32 known ones, were isolated from the root bark of Morus alba L. using various chromatographic methods. The structures of the undescribed compounds were elucidated based on 1D, 2D NMR, and HRESIMS dataanalysis, while the known ones were identified by comparison of their spectroscopic data with those reported in the literature. All the isolates were evaluated for their cytotoxic activities against human gastric cancer HGC27 cells by CCK-8 assay. Among them, compounds 5, 8, 10, and 30 exhibited cytotoxic activities on HGC27 cells with IC50 values of 33.76 ± 2.64 μM, 28.94 ± 0.72 μM, 6.08 ± 0.34 μM, and 10.24 ± 0.89 μM, respectively. Furthermore, compound 10 was confirmed to reduce proliferation ability, increase apoptosis rate, and inhibit cell migration pathway by annexin V/PI double staining experiment, transwell experiment, and Western blot analysis. Full article
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