Drug-Loaded Polymeric Micelles Based on Smart Biocompatible Graft Copolymers with Potential Applications for the Treatment of Glaucoma
Abstract
:1. Introduction
2. Results
2.1. Micellar Sizes and Stability
2.2. FTIR Spectroscopy
2.3. Assessment of the Haemolysis Degree
2.4. In Vitro Cytotoxicity Analysis
2.5. Drug Release Kinetics
2.6. In Vivo Evaluation of the IOP
3. Materials and Methods
3.1. Materials
3.2. Methods
3.2.1. Micelle Preparation Procedure
3.2.2. Physicochemical Characterisation Methods
3.2.3. In Vitro Biological Characterisation Methods
3.2.4. In Vivo Tests
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Sample | Z-Average (nm) | Dv (nm) | PDI | ZP (mV) | DEE (%) | DLE (%) |
---|---|---|---|---|---|---|
Cop A | 39.4 ± 0.1 | 24.2 ± 0.2 | 0.197 | −3.5 | - | - |
Cop A/Dorzolamide = 10/1 (wt/wt) | 41.3 ± 0.4 | 24.5 ± 0.5 | 0.258 | −3.3 | 20.2 | 4.4 |
Cop A/IMC = 10/1 (wt/wt) | 29.6 ± 0.3 | 23.0 ± 0.1 | 0.154 | −7.5 | 68.5 | 6.3 |
Cop B | 47.2 ± 0.2 | 34.8 ± 0.4 | 0.200 | −3.3 | - | - |
Cop B/Dorzolamide = 10/1 (wt/wt) | 54.8 ± 0.3 | 37.5 ± 0.2 | 0.354 | −3.3 | 34.0 | 6.7 |
Cop B/IMC = 10/1 (wt/wt) | 41.8 ± 0.4 | 31.0 ± 0.1 | 0.161 | −5.5 | 75.1 | 8.2 |
Cop B/Dorzolamide = 10/1+Cop B/IMC = 10/1 (50/50 wt/wt) | 45.7 ± 0.5 | 33.9 ± 0.2 | 0.252 | −4.1 | - | - |
Sample | 24 h | 48 h | |
---|---|---|---|
Control | |||
Cop B | 10 µg/mL | ||
50 µg/mL | |||
100 µg/mL | |||
Cop B/IMC = 10/1 (wt/wt) | 10 µg/mL | ||
50 µg/mL | |||
100 µg/mL | |||
Cop B/Dorzolamide = 10/1 (wt/wt) | 10 µg/mL | ||
50 µg/mL | |||
100 µg/mL |
Sample | IOP (mmHg) | |||||
---|---|---|---|---|---|---|
After 15 Days of DEX Administration | After 15 Days of Treatment | |||||
Morning | Noon | Evening | Morning | Noon | Evening | |
LC | 5 | 6 | 7 | 5 | 6 | 7 |
LE1 | 9 | 10 | 11 | 9 | 10 | 11 |
LE2 | 9 | 10 | 11 | 9 | 10 | 11 |
LE3 | 9 | 10 | 11 | 7 | 8 | 9 |
LE4 | 9 | 10 | 11 | 4 | 5 | 5 |
LE5 | 9 | 11 | 12 | 10 | 11 | 13 |
Copolymer | Sample Name | PNVCL (mol %) | PNVP (mol%) | DPn, Average per Graft |
---|---|---|---|---|
PCL120-g-P(NVCL507-co-NVP128) | Cop A | 64 | 20 | 42 |
PCL120-g-P(NVCL1253-co-NVP139) | Cop B | 82 | 10 | 93 |
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Ozturk, M.-R.; Popa, M.; Rata, D.M.; Cadinoiu, A.N.; Parfait, F.; Delaite, C.; Atanase, L.I.; Solcan, C.; Daraba, O.M. Drug-Loaded Polymeric Micelles Based on Smart Biocompatible Graft Copolymers with Potential Applications for the Treatment of Glaucoma. Int. J. Mol. Sci. 2022, 23, 9382. https://doi.org/10.3390/ijms23169382
Ozturk M-R, Popa M, Rata DM, Cadinoiu AN, Parfait F, Delaite C, Atanase LI, Solcan C, Daraba OM. Drug-Loaded Polymeric Micelles Based on Smart Biocompatible Graft Copolymers with Potential Applications for the Treatment of Glaucoma. International Journal of Molecular Sciences. 2022; 23(16):9382. https://doi.org/10.3390/ijms23169382
Chicago/Turabian StyleOzturk, Manuela-Ramona (Blanaru), Marcel Popa, Delia Mihaela Rata, Anca Niculina Cadinoiu, Frederique Parfait, Christelle Delaite, Leonard Ionut Atanase, Carmen Solcan, and Oana Maria Daraba. 2022. "Drug-Loaded Polymeric Micelles Based on Smart Biocompatible Graft Copolymers with Potential Applications for the Treatment of Glaucoma" International Journal of Molecular Sciences 23, no. 16: 9382. https://doi.org/10.3390/ijms23169382