Unveiling the Role of SlRNC1 in Chloroplast Development and Global Gene Regulation in Tomato Plants
Abstract
:1. Introduction
2. Results
2.1. Identification of RNC1 in Tomato and Sequence Alignment RNC1 Proteins across Four Plants
2.2. VIGS-Induced SlRNC1 Silencing Results in Dwarf Tomato Plants with Yellowish Leaves
2.3. Subcellular Localization of SlRNC1
2.4. Silencing of SlRNC1 Caused Ultrastructural Changes in Chloroplasts
2.5. Analysis of Differentially Expressed Genes Caused by SlRNC1 Silencing
2.6. Gene Ontology (GO) and KEGG Analysis of DEGs
2.7. qRT-PCR Confirmation of DEGs
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Agrobacterium Transformation-Mediated Transient Gene Expression
4.3. RNA Extraction, RT-PCR, and RT-qPCR
4.4. Plasmid Construction and Inoculation
4.5. SlRNC1 Gene Identification, Sequence Alignment, and Protein Structure Modeling
4.6. Subcellular Localization Analysis and TEM Analysis
4.7. RNA-Seq Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Samples | Libraries | Raw_Reads | Raw_Bases | Clean_Reads | Clean_Bases | Error_Rate | Q20 | Q30 | GC_pct |
---|---|---|---|---|---|---|---|---|---|
TRV:SlRNC1-1 | SRAS240002246-1a | 47923554 | 7.19G | 46986592 | 7.05G | 0.01 | 98.80 | 96.58 | 43.48 |
TRV:SlRNC1-2 | SRAS240002247-1a | 50772296 | 7.62G | 49650698 | 7.45G | 0.01 | 98.24 | 95.70 | 41.04 |
TRV:SlRNC1-3 | SRAS240002248-1a | 46462328 | 6.97G | 43268944 | 6.49G | 0.01 | 98.83 | 96.70 | 43.41 |
TRV:00-1 | SRAS240002249-1a | 44465486 | 6.67G | 41516244 | 6.23G | 0.01 | 98.67 | 96.26 | 42.58 |
TRV:00-2 | SRAS240002250-1a | 44624792 | 6.69G | 44557430 | 6.68G | 0.01 | 98.53 | 95.86 | 43.27 |
TRV:00-3 | SRAS240002251-1a | 45080082 | 6.76G | 43849266 | 6.58G | 0.01 | 98.80 | 96.62 | 42.41 |
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Nie, Y.; Zhang, Y.; Wang, L.; Wu, J. Unveiling the Role of SlRNC1 in Chloroplast Development and Global Gene Regulation in Tomato Plants. Int. J. Mol. Sci. 2024, 25, 6898. https://doi.org/10.3390/ijms25136898
Nie Y, Zhang Y, Wang L, Wu J. Unveiling the Role of SlRNC1 in Chloroplast Development and Global Gene Regulation in Tomato Plants. International Journal of Molecular Sciences. 2024; 25(13):6898. https://doi.org/10.3390/ijms25136898
Chicago/Turabian StyleNie, Yuxin, Yuhong Zhang, Luyou Wang, and Jian Wu. 2024. "Unveiling the Role of SlRNC1 in Chloroplast Development and Global Gene Regulation in Tomato Plants" International Journal of Molecular Sciences 25, no. 13: 6898. https://doi.org/10.3390/ijms25136898