Matrine Suppresses Arsenic-Induced Malignant Transformation of SV-HUC-1 Cells via NOX2
Abstract
:1. Introduction
2. Results
2.1. Effect of Long-Term Arsenite Exposure on Proliferation in SV-HUC-1 Cells
2.2. Effect of Long-Term Arsenite Exposure on Migration and Angiogenesis in SV-HUC-1 Cells
2.3. Chronic Arsenite Treatment Activates the Expression of NLRP3 Inflammasome and NOX2 in SV-HUC-1 Cells
2.4. Arsenite-Induced Overexpression of NLRP3 Inflammasome and NOX2 Decreased by Matrine In Vitro
2.5. Matrine Suppressed the Expression of NLRP3 Inflammasome via Inhibiting NOX2 In Vivo
2.6. Matrine Decreased the Proliferation and Cell Viability in Bladder Epithelial Cells
2.7. Matrine Regulated E-Cadherin and Vimentin and Diminished the Ability of Migration in Bladder Epithelial Cells
2.8. Matrine Declined the Angiogenesis in Bladder Epithelial Cells
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Reagents and Antibodies
4.3. Western Blot Analysis
4.4. RNA Extraction and Real-Time Quantitative PCR
4.5. Cell Viability Analysis
4.6. Wound Healing Analysis
4.7. Cell Cycle Assay
4.8. Reactive Oxygen Species Assay
4.9. Animals and Treatment
4.10. Immunohistochemistry
4.11. Immunofluorescence
4.12. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene | Forward Primer (5′-3′) | Reverse Primer (5′-3′) |
---|---|---|
Cyclin D1 | GCTGCGAAGTGGAAACCATC | CCTCCTTCTGCACACATTTGAA |
PCNA | GGCCGAAGATAACGCGGATAC | GGCATATACGTGCAAATTCACCAG |
E–cadherin | GCTGGACCGAGAGAGTTTCC | CAAAATCCAAGCCCGTGGTG |
Vimentin | ACCAGCTAACCAACGACAAAG | AAAGATTGCAGGGTGTTTTCG |
VEGF | CCGCAGACGTGTAAATGTTCCT | TTCCGGTGAGAGGTCTGGTTC |
NLRP3 | GCGTGGTCTTGAATTCCTCA | GGCACACGGATGAGTCTTT |
IL–1β | GCCAGTGAAATGATGGCTTATT | AGGAGCACTTCATCTGTTTAGG |
IL–18 | GCTGAAGATGATGAAAACCTGG | CAAATAGAGGCCGATTTCCTTG |
Caspase 1 | CATCCCACAATGGGCTCTGT | GCATCTGCGCTCTACCATCT |
NOX2 | AAGATGCGTGGAAACTACCTAA | TTTTTGAGCTTCAGATTGGTGG |
GAPDH | TGTTGCCATCAATGACCCCTT | CTCCACGACGTACTCAGCG |
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Wang, L.; Qiu, N.; Tong, S.; Yu, Y.; Xi, S.; Wang, F. Matrine Suppresses Arsenic-Induced Malignant Transformation of SV-HUC-1 Cells via NOX2. Int. J. Mol. Sci. 2024, 25, 8878. https://doi.org/10.3390/ijms25168878
Wang L, Qiu N, Tong S, Yu Y, Xi S, Wang F. Matrine Suppresses Arsenic-Induced Malignant Transformation of SV-HUC-1 Cells via NOX2. International Journal of Molecular Sciences. 2024; 25(16):8878. https://doi.org/10.3390/ijms25168878
Chicago/Turabian StyleWang, Lanfei, Nianfeng Qiu, Suyuan Tong, Yan Yu, Shuhua Xi, and Fei Wang. 2024. "Matrine Suppresses Arsenic-Induced Malignant Transformation of SV-HUC-1 Cells via NOX2" International Journal of Molecular Sciences 25, no. 16: 8878. https://doi.org/10.3390/ijms25168878