Impact of Airborne Exposure to PM10 Increases Susceptibility to P. aeruginosa Infection
Abstract
:1. Introduction
2. Materials and Methods
2.1. Mice
2.2. Whole-Body Exposure to PM10
2.3. SKQ1 and Moxifloxacin Treatment
2.4. Bacterial Culture and Corneal Infection
2.5. Ocular Response to Bacterial Infection
2.6. RT-PCR
2.7. Western Blot
2.8. Bacterial Plate Count
2.9. P. aeruginosa and SKQ1 Killing
2.10. Myeloperoxidase (MPO) Assay
2.11. Statistical Analysis
3. Results
3.1. Clinical Score and Slit lamp
3.2. RT-PCR
3.3. Western Blot
3.4. Viable Plate Count and MPO Assay
3.5. SKQ1 Antibiotic Effect
3.6. Clinical Score and Slit Lamp after Moxifloxacin/SKQ1 Treatment
3.7. Viable Plate Count and MPO Assay
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene | Nucleotide Sequence | Primer | GenBank |
---|---|---|---|
18s | 5’-GTA ACC CGT TGA ACC CCA TT-3’ | F | NM_003278.3 |
5’-CCA TCC AAT CGG TAG TAG CG-3′ | R | ||
Inf-a | 5’-ACC CTC ACA CTC AGA TCA TCT T -3′ | F | NM_013693.2 |
5’-GGT TGT CTT TGA GAT CCA TGC -3′ | R | ||
I1-1β | 5’-CGC AGC AGC ACA TCA ACA AGA GC -3′ | F | NM_008361.3 |
5′-TGT CCT CAT CCT GGA AGG TCC ACG -3’ | R | ||
Cxc12 | 5′-TGT CAA TGC CTG AAG ACC CTG CC -3′ | F | NM_009140.2 |
5′-AAC TTT TTG ACC GCC CTT GAG AGT GG -3’ | R | ||
Tlr4 | 5′-CCT GAC ACC AGG AAG CTT G AA -3′ | F | NM_021297.2 |
5′-TCT GAT CCA TGC ATT GGT AGG T -3′ | |||
I1-6 | 5′-CAC AAG TCC GGA GAG GAG AC-3′ | F | NM_031168.1 |
5′-CAG AAT TGC CAT TGC ACA AC-3′ | R | ||
II-10 | 5′-TGC TAA CCG ACT CCT TAA TGC AGG AC-3′ | F | NM_010548.2 |
5′-CCT TGA TTT CTG GGC CAT GCT TCT C-3′ | R | ||
Cox2 | 5′-GCA GTT CCA GTA TCA GAA CCG CAT TG-3′ | F | NM_011198.2 |
5′-GAG TGA GTC CAT GTT CCA GGA GGA TG-3’ | R | ||
inos | 5′-TCC TCA CTG GGA CAG CAC AGA ATG-3′ | F | NM_010927.3 |
5′-GTG TCA TGC AAA ATC TCT CCA CTG CC-3′ | R | ||
Nqo1 | 5′-TTC TGT GGC TTC CAG GTC TT-3′ | F | NM_008706.5 |
5′-TCC AGA CGT TTC TTC CAT CC-3′ | R | ||
Gr1 | 5′-CCA CGG CTA TGC AAC ATT CG-3′ | F | NM_010344.4 |
5′-GAT CTG GCT CTC GTG AGG AA-3′ | R | ||
Gpx4 | 5′-GCA ACC AGT TTG GGA GGC AGG AG-3′ | F | NM_008162.4 |
5′-CCT CCA TGG GAC CAT AGC GCT TC-3′ | R | ||
Nrf2 | 5′-TGC CCC TCA TCA GGC CCA GT-3′ | F | NM_010902.5 |
5′-GCT CGG CTG GGA CTC GTG TT-3′ | R |
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McClellan, S.A.; Wright, R.; Muhammed, F.; Hazlett, L.D. Impact of Airborne Exposure to PM10 Increases Susceptibility to P. aeruginosa Infection. Int. J. Environ. Res. Public Health 2024, 21, 722. https://doi.org/10.3390/ijerph21060722
McClellan SA, Wright R, Muhammed F, Hazlett LD. Impact of Airborne Exposure to PM10 Increases Susceptibility to P. aeruginosa Infection. International Journal of Environmental Research and Public Health. 2024; 21(6):722. https://doi.org/10.3390/ijerph21060722
Chicago/Turabian StyleMcClellan, Sharon A., Robert Wright, Farooq Muhammed, and Linda D. Hazlett. 2024. "Impact of Airborne Exposure to PM10 Increases Susceptibility to P. aeruginosa Infection" International Journal of Environmental Research and Public Health 21, no. 6: 722. https://doi.org/10.3390/ijerph21060722