AMPKα1 Regulates Lung and Breast Cancer Progression by Regulating TLR4-Mediated TRAF6-BECN1 Signaling Axis
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Study Population
2.2. Cell Lines and Cell Culture
2.3. Antibodies and Reagents
2.4. Generation of AMPKα1-Knockdown (AMPKα1KD) THP-1 Cells
2.5. Generation of AMPKαl-Knockout (AMPKα1KO) Cancer Cell Lines With CRISPR/Cas9
2.6. Microarray Analysis
2.7. Plasmid Constructs
2.8. Western Blotting Analysis and Immunoprecipitation (IP) Assays
2.9. Wound-Healing and Transwell Migration Assay
2.10. Immunofluorescence Microscopy
2.11. GEPIA Data Analysis
2.12. Statistical Analysis
3. Results
3.1. AMPKα1 is Functionally Associated With TRAF6 and BECN1
3.2. AMPKα1 Enhances Ubiquitination of BECN1 and Regulates Autophagy Induction in Serum Starvation Condition
3.3. AMPKα1-Knockdown THP-1 Cells Show Impaired Autophagy Flux by TLR4 Stimulation
3.4. AMPKα1 is Positively Associated With Autophagy in AMPKα1-Knockout A549 Human Lung Cancer and Primary-NSCLCs
3.5. AMPKα1-Knockout MDA-MB-231 and MCF-7 Breast Cancer Cells Show Attenuated Cancer Migration and Invasion
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Kim, M.-J.; Min, Y.; Son, J.; Kim, J.Y.; Lee, J.S.; Kim, D.-H.; Lee, K.-Y. AMPKα1 Regulates Lung and Breast Cancer Progression by Regulating TLR4-Mediated TRAF6-BECN1 Signaling Axis. Cancers 2020, 12, 3289. https://doi.org/10.3390/cancers12113289
Kim M-J, Min Y, Son J, Kim JY, Lee JS, Kim D-H, Lee K-Y. AMPKα1 Regulates Lung and Breast Cancer Progression by Regulating TLR4-Mediated TRAF6-BECN1 Signaling Axis. Cancers. 2020; 12(11):3289. https://doi.org/10.3390/cancers12113289
Chicago/Turabian StyleKim, Mi-Jeong, Yoon Min, Juhee Son, Ji Young Kim, Ji Su Lee, Duk-Hwan Kim, and Ki-Young Lee. 2020. "AMPKα1 Regulates Lung and Breast Cancer Progression by Regulating TLR4-Mediated TRAF6-BECN1 Signaling Axis" Cancers 12, no. 11: 3289. https://doi.org/10.3390/cancers12113289