Agronomy, Volume 13, Issue 9 (September 2023) – 255 articles

Water deficit is a significant abiotic stress affecting crop growth and production. While many studies have indicated that salicylic acid (SA) plays a crucial role in mitigating the detrimental effects of environmental stress on plants, its mechanism regulating the photosynthetic adaptability of maize seedlings under water deficit is still unclear. This study aimed to investigate the impact of exogenous SA on maize seedling performance under polyethylene glycol (PEG)-induced water deficit. The results showed that PEG treatment destroyed the integrity of chloroplast and reduced chlorophyll content and photosynthesis rate (Pn), leading to growth retardation of maize seedlings with lower biomass accumulation and leaf relative water content (RWC). Moreover, chlorophyll fluorescence index, including potential photochemical activity (Fv/Fo), maximum Photosystem II (PSII) quantum yield (Fv/Fm), and energy captured by PSII reaction center for electron transfer (Eto/RC), were decreased, but energy dissipated by unit reaction center (DIo/RC) was enhanced in maize seedlings under water deficit. In addition, PEG treatment also significantly declined the activity of Rubisco and Rubisco activase (RCA) in maize seedlings. In contrast, SA treatment enhanced the content of chlorophyll, as well as the transcription level of psbA, and RCA and Rubisco small subunit (rbcS) reduced the damaging effects of PEG treatment by protecting the integrity of chloroplast and repairing the damaged PSII reaction center, thus positively regulating photosynthetic reaction and water-deficit tolerance in maize seedlings. Our data implied that SA played an important regulatory role in plant resistance to water-deficit stress, and the result will further supply the regulatory network of SA-mediated photosynthetic adaptability. Full article

The effective exploration and utilization of brackish water resources are crucial to alleviating the scarcity of freshwater in arid regions. This study focused on protected tomato plants and set up four irrigation salinity levels: T1 (2 g·L⁻¹), T2 (4 g·L⁻¹), T3 (6 g·L⁻¹), and T4 (8 g·L⁻¹), with freshwater irrigation as a control (CK). The aim was to investigate the effects of continuous brackish water irrigation on soil water-salt transport and tomato yield. The outcomes highlighted that the moisture content in different layers of soil exhibited a “high in the middle, low at both ends” pattern, with the primary accumulation of soil moisture occurring at the 40 cm depth. The range and moisture content of the soil wetted zone increased with elevated salinity levels. Under continuous brackish water irrigation, the range of the soil wetted zone expanded further for the autumn crops, and the moisture content significantly increased compared to the spring crops. The concentration of soil salt gradually decreased with increasing soil depth, exhibiting greater levels in the 0–20 cm layer compared to the 40–80 cm layer. The average salt concentration in the soil at the end of the growth period was significantly higher than before transplantation, and this phenomenon became more pronounced with increasing salinity levels. Initial irrigation with brackish water with a salinity level of 2–4 g·L⁻¹ promoted the growth of the tomatoes planted in the spring and the plant height and stem diameter reached the peak values of 1.68 m and 1.08 mm for the T2 treatment, respectively, which were 7.1% and 9.2% higher than that of the CK treatment, ensuring efficient yield and water usage. However, continuous irrigation with brackish water with a salinity level of 2–4 g·L⁻¹ inhibited the growth and yield of the tomatoes planted in autumn, while the T1 and T2 treatments only yielded 24,427.42 and 16,774.86 kg·hm⁻², respectively, showing a decline of 32.2% and 46.1% compared to the yields of the spring season. Considering the soil water-salt and yield indicators, under the conditions of non-continuous brackish water irrigation, using water with a salinity level of 2–4 g·L⁻¹ is recommended for drip irrigation of protected tomatoes. Full article

Going beyond previous work, this paper presents a systematic literature review that explores the deployment of satellites, drones, and ground-based sensors for yield prediction in agriculture. It covers multiple aspects of the topic, including crop types, key sensor platforms, data analysis techniques, and performance in estimating yield. To this end, datasets from Scopus and Web of Science were analyzed, resulting in the full review of 269 out of 1429 retrieved publications. Our study revealed that China (93 articles, >1800 citations) and the USA (58 articles, >1600 citations) are prominent contributors in this field; while satellites were the primary remote sensing platform (62%), followed by airborne (30%) and proximal sensors (27%). Additionally, statistical methods were used in 157 articles, and model-based approaches were utilized in 60 articles, while machine learning and deep learning were employed in 142 articles and 62 articles, respectively. When comparing methods, machine learning and deep learning methods exhibited high accuracy in crop yield prediction, while other techniques also demonstrated success, contingent on the specific crop platform and method employed. The findings of this study serve as a comprehensive roadmap for researchers and farmers, enabling them to make data-driven decisions and optimize agricultural practices, paving the way towards a fully digitized yield prediction. Full article

Biochar is a potential solution for addressing environmental problems related to excessive nitrogen (N). However, there is still some debate about the absorption and desorption of nitrate nitrogen (NO₃⁻-N). Therefore, this study investigated the NO₃⁻-N adsorption and desorption performance onto biochar and biochar-soil mixture to address this gap. The results showed that the biochar produced from apple branches had the ability to absorb NO₃⁻-N with an absorption capacity of 3.51 mg·g⁻¹. The absorption data fitted well with the pseudo-second-order kinetic model and Langmuir model. The application of biochar significantly improved soil absorption capacity and slow release of NO₃⁻-N. While higher NO₃⁻-N concentrations had better NO₃⁻-N supply capacity and poorer slow-release effect. Integrating nutrient supply and slow-release effect, it is recommended to control the application ratio of biochar to NO₃⁻-N at 34–42.75 g·g⁻¹. Although the unoptimized biochar application rate cannot be directly applied to the soil as a slow-release fertilizer carrier to meet commercial standards, biochar modification provides new possibilities for this purpose. Moreover, compared with traditional slow-release fertilizer, biochar had good stability and regeneration performance, alleviating the high cost due to the biochar price. In general, biochar still has potential and prospects as a slow-release material. This study provides support for biochar in mitigating environmental problems associated with excess N. Full article

Similar to many other plant-based products, strawberries are susceptible to fungal diseases caused by various pathogen groups. In recent years, efforts have been made to combat these diseases using biological control methods, particularly the application of arbuscular mycorrhizal fungi (AMF). This study aimed to determine the effects of AMF (Funneliformis mosseae (Fm) and Gigaspora margarita (Gm)) on Rhizoctonia fragariae (Rf), Fusarium oxysporum (Fo), and Alternaria alternata (Aa), which are major pathogens for strawberry. The results showed that the effects of AMF on disease severity and plant growth varied depending on the pathogens involved. Rf caused the highest disease severity, followed by Fo and Aa, but all AMF treatments significantly reduced the disease severity compared to control treatments. The study also found that the specific AMF species and their combinations influenced plant growth responses under different pathogenic conditions. Different AMF treatments resulted in varying increases in plant fresh weight, dry weight, and length, depending on the pathogen. Moreover, the application of AMF led to increased levels of total phenolic content, antioxidant activity, and phosphorus content in pathogen-infected plants compared to control treatments. Fm was more efficient than Gm in increasing these biochemical parameters. The levels of root colonization by AMF were similar among different AMF treatments, but the effects on fungal spore density varied depending on the pathogen. Some AMF treatments increased fungal spore density, while others did not show significant differences. In conclusion, our research sheds light on the differential effects of AMF species on disease severity, plant growth, and biochemical parameters in strawberry plants facing diverse pathogens. These findings underscore the potential benefits of AMF in disease management, as they reduce disease severity and bolster plant growth and defense mechanisms. Full article

M. bourgaei Baker is an endemic plant that grows in Türkiye. It has the potential to be an ornamental plant, so it is important to know its germination characteristics and seed morphological characteristics. We evaluated the effects of moist chilling (3 to 12 months) and application of gibberellic acid (GA₃) (250 to 1000 mg/L⁻¹) on seed germination under two different light regimes (light phase and dark phase) and four temperature regimes (20, 20/10, 25/15, and 15/10 °C). Seeds were collected from the subalpine belt of Mount Uludağ at an altitude between 2200 and 2300 m. M. bourgaei seeds were dormant and reached the highest germination percentage after 12 months of moist chilling. GA₃ applications only have a limited effect on the breaking of dormancy in most cases. Maximum germination of 28 percent was obtained by the GA₃ application of 1000 mg/L under dark phase with incubation at 15/10 °C. Scarification with sulfuric acid did not result in any germination. Seed germination of above 80 percent was obtained after 6 months of moist chilling and above 90 percent after 9 and 12 months of moist chilling. Intermediate physiological dormancy was determined as the type of dormancy. Our findings on seed germination and dormancy characteristics of M. bourgaei, which is an endemic alpine meadow plant, will contribute to the protection and development of the germplasm of this species. Full article

Fusarium wilt, caused by Fusarium oxysporum f. sp. niveum (FON), is a predominant and devastating soil-borne disease that results in significant yield losses in watermelon cultivation. In this study, a strain MM isolated from the herbage rhizosphere soil, exhibited an inhibition rate of 65.46% against FON, leading to mycelial collapse, atrophy, and deformation. In pot experiments, strain MM effectively controlled Fusarium wilt of watermelon, showing a control efficacy of 74.07%. Through morphological observation and 16S rDNA gene sequencing, strain MM was identified as Serratia plymuthica. Additionally, S. plymuthica MM demonstrated antagonistic activity against eight plant pathogens, indicating that MM had broad-spectrum antifungal activity. The strain also exhibited the ability to synthesize siderophores and indole acetic acid (IAA), both of which are growth-promoting compounds. Moreover, strain MM secreted various extracellular enzymes, including protease, chitinase, β-glucanase, and cellulase. This ability allowed S. plymuthica MM to readily colonize watermelon roots and promote seedling growth. Inoculation with S. plymuthica MM increased the activity of PAL, POD, PPO, and CAT enzymes associated with watermelon defense. Furthermore, qRT-PCR analysis revealed up-regulation of LOX, POD, PAL, ClPR3, and C4H genes, which are related to plant disease resistance. The results indicated that S. plymuthica MM enhances watermelon plants’ resistance to FON by activating the JA, SA, and shikimic acid phenylpropanoid–lignin synthesis pathways. Gas chromatography–mass spectrometry (GC-MS) analysis of S. plymuthica MM culture supernatant identified piperazinedione, pyrrolo[1,2-a]pyrazine-1,4-dione, and octadecenamide as the main antimicrobial substances. Overall, S. plymuthica MM shows promise as a biocontrol agent against Fusarium wilt of watermelon, suggesting its potential for the development of a new biocontrol agent. Full article

Phosphorus (P) leaching from excessive P application is the primary pathway of P losses in agricultural soils. Different P fertilizer practices have mixed effects on P leaching. We conducted a meta-analysis of the relevant literature regarding the response of crop yields, soil-available P (AP), and total P (TP) leaching to reduced P input (RP) and an inorganic-organic combination fertilizer (NPKM) for different agricultural land-use types. Compared to conventional P application (CP), RP (10~90% reduction) did not reduce crop yields in vegetable fields (experiments were 1~4 years) but significantly reduced cereal yields by 4.57%. Compared to chemical fertilizer (NPK), NPKM significantly increased cereal yields by 12.73%. Compared to CP, RP significantly reduced AP at 0~60 cm in vegetable and cereal fields. The greatest reduction occurred at 20~40 cm in vegetable fields (40.29%) and 0~20 cm in cereal fields (34.45%). Compared to NPK, NPKM significantly increased the AP at 0~60 cm in vegetable fields, with the greatest increase (52.44%) at 20~40 cm. The AP at 0~40 cm in cereal fields significantly increased under the NPKM treatment, with the greatest increase at 0~20 cm (76.72%). Compared to CP, RP significantly decreased TP leaching by 16.02% and 31.50% in vegetable and cereal fields, respectively. Compared to NPK, NPKM significantly increased TP leaching in vegetable fields (30.43%); no significant difference in leaching occurred in cereal fields. P leaching, in response to RP, was influenced by the P amounts applied (34.49%); soil organic matter (14.49%); and TP (12.12%). P leaching in response to NPKM was influenced by multiple factors: rainfall (16.05%); soil organic matter (12.37%); soil bulk density (12.07%); TP (11.65%); pH (11.41%). NPKM was more beneficial for improving yields in cereal fields with low soil fertility and lower P-leaching risks. Full article

The automated harvesting of clustered fruits relies on fast and accurate visual perception. However, the obscured stem diameters via leaf occlusion lack any discernible texture patterns. Nevertheless, our human visual system can often judge the position of harvesting points. Inspired by this, the aim of this paper is to address this issue by leveraging the morphology and the distribution of fruit contour gradient directions. Firstly, this paper proposes the calculation of fruit normal vectors using edge computation and gradient direction distribution. The research results demonstrate a significant mathematical relationship between the contour edge gradient and its inclination angle, but the experiments show that the standard error projected onto the Y-axis is smaller, which is evidently more conducive to distinguishing the gradient distribution. Secondly, for the front view of occluded lychee clusters, a fully convolutional, feature prototype-based one-stage instance segmentation network is proposed, named the lychee picking point prediction network (LP³Net). This network can achieve high accuracy and real-time instance segmentation, as well as for occluded and overlapping fruits. Finally, the experimental results show that the LP³Net based on this study, along with lychee phenotypic features, achieves an average location accuracy reaching 82%, significantly improving the precision of harvesting point localization for lychee clusters. Full article

Atriplex canescens is widely cultivated as drought and salt-tolerant fodder in arid regions of Northwest China, which is used for photoremediation of degraded land and soil and water conservation. To explore the growth performance of A. canescens when exposed to drought and salt stress, seedlings were treated with a range of drought stress (WC1: 75 ± 3.6%, WC2: 49 ± 2.9% and WC3: 27 ± 2.5% of soil water content) and the corresponding drought stress with additional sodium salt supplementation (NaCl:Na₂SO₄ = 1:1 with the total concentration of Na⁺ set to 150 mM). The findings of this paper indicated that moderate sodium salt could stimulate the growth of A. canescens and effectively alleviate the deleterious impact of drought stress by increasing the turgor potential (ψt) and relative water content (RWC) and decreasing the leaf water osmotic potential (ψs). Furthermore, the photosynthetic capacity was improved and the negative effects of drought stress on photosystem II (PSII) were mitigated. The extra 150 mM sodium salt also markedly increased the contribution of Na⁺ to ψs and the contribution of betaine to ψs. In summary, these results indicate that A. canescens can adapt to drought stress by accumulating enough Na⁺ for osmotic adjustment (OA). Additionally, this paper is aimed to provide a fundamental basis for the utilization and cultivation of A. canescens as a favored pasture crop in the Qaidam basin, thus increasing the ecological and environmental benefits for arid regions worldwide. Full article

To explore the multipurpose utilization of tobacco germplasm resources, 80 representative air/sun-cured tobacco germplasms were used as experimental materials to determine the composition and content of the diterpenoids of glandular trichome secretions using ultra-performance liquid chromatography (UPLC). Correlation analysis, cluster analysis, and principal component analysis (PCA) were used to comprehensively evaluate 80 air/sun-cured tobacco germplasms. The results are as follows: (1) 11 chromatographic peaks (Peak1–Peak11) were detected by UPLC, and the coefficient of variation of their contents ranged from 26.3 to 143%. (2) Correlation analysis showed that alpha-cembratriene-diol (α-CBT-diol) and beta-cembratriene-diol (β-CBT-diol) had the highest correlation coefficients (0.97), showing a significantly negative correlation with cis-abienol and a significantly positive correlation with the other diterpenoids. (3) PCA showed that the cumulative contribution rate of the three principal components was 75.70%, and the diterpenoid-rich germplasms were ranked in the following order: X40 (Maiduo) > X48 (Jianpingpiaoba) > X58 (Mianzhu) > X54 (Shifangpipaliu) > X63 (Xuejia5) > X41 (Tangpeng) > X29 (OLOR) > X73 (Criollo) > X44 (Tiebanqing) > X70 (Nicaragua Changxin). The diterpenoid content of X36 was the lowest. These results provide excellent germplasm for the extraction and exploitation of the diterpenoids and for genetic studies of their metabolism. Full article

As part of the ecological barrier and an essential element of food security, the agro-pastoral ecotone is vital in northern China. Since soil fertility in northern China is low due to frequent surface disturbances, it is necessary to improve the properties of the soil. This study aims to examine the impact of fungal residue return on soil properties based on six treatments (CK: 0 kg/40 m²; R3: 90 kg/40 m²; R5: 150 kg/40 m²; R7: 210 kg/40 m²; R9: 270 kg/40 m²; R11: 330 kg/40 m²;) of fungal residue return concentration experimental data from 0 to 30 cm soil depth. The results showed that the effect of fungal residue returning on soil habits was greater at 0–10 cm of the surface layer. The bulk density can be reduced to 25.83% of CK, and water content can be increased up to 26.26%. Adding fungal residue to the field led to a greater increase in soil parameters (SOM and AP), and this characteristic effect continued as the return concentration increased. The number of soil bacteria and actinomycetes remained stable, and the amount of fungi was at its lowest. Compared with CK, the number of bacteria, fungi, and actinomycetes increased by 1.94 times, 1.46 times, and 1.71 times, respectively. After the residue was returned to the field, AK had the strongest correlation with other factors (p < 0.01), and microorganism and enzyme activities were strongly correlated (p < 0.01). In conclusion, this study presents a new method of resource utilization of downstream wastes in the food industry while simultaneously providing natural, pollution-free improvements to the soil, which is very beneficial to increasing crop yield. Full article

The Thompson Seedless (Sultanina) seedless variety of grapes is an important crop in Egypt, both for local consumption and export. In recent years, the H4 strain of this grape variety has gained popularity due to its high productivity. However, a drawback of this strain is that the grape clusters become densely packed, resulting in small berries and reduced overall quality. A study was conducted to investigate the impact of pruning severity and bud load on the growth, yield, and quality of H4 grapes. The study included several different treatments, namely T1 Control: (10 canes × 12 buds), T2: (8 canes × 6 buds), T3: (8 canes × 8 buds), T4: (8 canes × 10 buds), T5: (8 canes × 12 buds), T6: (6 canes × 13 buds), T7: (8 canes × 13 buds), T8: (10 canes × 13 buds), and T9: (12 canes × 13 buds). The behavior of the buds, vegetative growth, yield, and quality of the grapes were analyzed. The results showed that pruning of the H4 strain with either 8 canes and 10–12 buds per cane, or 6 canes with 13 buds, produced the best results in terms of managing excessive fruit production, achieving a balance between vegetative growth and yield, and improving the physical and chemical characteristics of the grape clusters and berries. Overall, maintaining 8 canes with 10–12 buds per cane or 6 canes with 13 buds is recommended for obtaining maximum crop yield and quality. Full article

Improving yield in potato production with minimal environmental impact is of great significance for China’s potato staple food policy. Previous research has been limited by the absence of regional-scale parameters to evaluate the environmental costs of regional potato production. To address this gap, we utilized the input–output analysis method to offer a thorough estimation of nitrogen (N), phosphorus (P), and potassium (K) inputs and outputs in the potato production stage at a regional scale, leveraging a meta-analysis dataset from plenty of the literature. On this basis, we calculated the virtual N, P, and K factors (VNFs, VPFs, and VKFs) for different potato production regions, under both conventional and optimal management practices. China’s potato production suffered from excessive N and P inputs, while K inputs remained insufficient. Significant spatial heterogeneities were observed for the VNFs, VPFs, and VKFs across different potato production regions. Northeast China and northwest China emerged as the most suitable potato cultivation regions because they demonstrated high potato yields with relatively low inputs and, consequently, lower VNFs and VPFs. Southwest China was the most vital region where targeted efforts could lead to reducing VNF and VPF, thus significantly mitigating environmental N and P losses. In addition to reducing fertilizer inputs, site-specific and whole optimization measures are proposed to lower the environmental costs and promote the sustainable development of potato production. Full article

Soya, one of the most economically important crops, is sensitive to periodic water deficits, which, because of climate change, are becoming more and more common in central Europe. The goal of this study was to estimate the water requirements of soybean from 1981 to 2020 in four provinces of central Poland and the rainfall deficits affecting soybean cultivation; the study also evaluates rainfall water use efficiency for soybean cultivated in production fields to investigate the future necessity of supplemental irrigation. Calculations were based on the values of monthly air temperature and the sum of precipitation. Soybean water requirements were calculated using the method of crop coefficients and reference evapotranspiration was estimated using the Blaney–Criddle approach. Crop water requirements were defined as potential evapotranspiration. Precipitation deficits for soybean were estimated using the Ostromęcki method. Water use efficiency was calculated based on rainfall totals and soybean yield in the rainfed fields. It was found that, on average in the central Poland provinces, from 1981 to 2020, the water requirements of soybean in the growing season amounted to 384 mm, and the highest water requirements occurred in June and July. In the studied forty-year period, a significant upward trend in soybean water requirements in central Poland was observed, both from 1 May to 31 August and from 1 June to 31 August. Rainfall deficits in soybean cultivation in central Poland were found from May to August and amounted to 123 mm in normal years (N_50%). The rainfall water use efficiency from April to August for soybean cultivated in central Poland on average amounted to 6.6 kg ha⁻¹ mm⁻¹ and varied in individual years and regions. The results of the study indicate the need to develop supplemental irrigation systems for soybean crops cultivated in central Poland and other areas of the world with similar climate conditions to optimize yield and the sustainable use of water resources. Full article

Appropriate deep application of fertilizer is the key basis for improving nitrogen use efficiency (NUE). However, the effects of different deep application methods and fertilizer types on nutrient migration, NUE and biomass in wheat season are unclear. Therefore, in this study, a barrel planting test with multilayer fertilization (¹⁵N labeled urea (U) and coated urea (CU)) was conducted in a long-term positioning trial of winter wheat in the North China Plain (NCP). We quantified the migration of fertilizer N (N_dff) in soil–plant–atmosphere and its effects on wheat biomass and NUE based on surface (U_sur, CU_sur), layered-strip (U_str, CU_str) and layered-mix fertilization (U_mix, CU_mix) of U and CU. Compared with surface fertilization, the concentration of mineral N in root zone (0–40 cm) was increased by U_str and U_mix (8.6–50.3%), and the concentration of ammonium N was decreased by CU_str and CU_mix (49.6–76.0%), but there was no change in the nitrate N. The biomass and total N absorption of wheat tissues (straw and root) were increased by 12.3–38.9% under U_str and CU_str. Meanwhile, the distribution of N_dff in the 0–10 cm soil was decreased under U_str and CU_str, but it was increased in the 10–30 cm soil, thereby promoting the absorption of N_dff in wheat tissues by 12.3–28.7%. The rates of absorption and loss of N_dff were the highest (57.6–58.5%) and the lowest (4.5%) under U_str and CU_str, respectively, compared with other treatments. Consequently, layered-strip fertilization optimized the migration and utilization of N_dff within the soil–plant–atmosphere system. This approach equalized distribution, enhanced absorption and minimized losses of N_dff, resulting in an increase in NUE by 9.6–16.7%. Under the same treatment, CU was more suitable for crop nutrient requirements than U, which was more conducive to the improvement of NUE. Our findings will provide a scientific basis for the precise directional fertilization of winter wheat in the NCP. Full article

Developing effective regulatory strategies to enhance irrigation water and fertilizer efficiency in the southern Xinjiang region of China, while simultaneously combatting desertification, is of paramount significance. This study focuses on Chinese jujube in Xinjiang and presents findings from a two-year field experiment aimed at investigating the optimal application strategy of microbial organic fertilizer (MOF). The research aims to provide a scientific foundation for achieving high-quality jujube production. The experiment involved a control group (utilizing only freshwater, referred to as CK) and various combinations of MOF treatments. In 2021, these treatments included M1 (0.6 t/ha), M2 (1.2 t/ha), M3 (1.8 t/ha), and M4 (2.4 t/ha), while in 2022, they encompassed M1 (0.6 t/ha), M2 (1.2 t/ha), M4 (2.4 t/ha), and M5 (4.8 t/ha). Over the two-year trial period, we assessed various indices, including the soil’s physical properties, hydraulic characteristics, soil enzyme activities, and relative chlorophyll content. Additionally, we evaluated jujube yield, quality, and economic benefits. The results indicate that MOF application led to significant improvements in soil conditions. Specifically, the average moisture content and profile water storage of the 0–50 cm soil layer increased by 10.98% to 36.42% and 1.8% to 26.8%, respectively. Moreover, in both the 2021 and 2022 experiments, soil saturated water content (SSWC) and water-holding capacity (WHC) increased by 6.25% to 15.98%, while soil hydraulic conductivity (Ks) and bulk density (BD) decreased by 2.91% to 9.88% and 0.63% to 8.08%, respectively. In 2021, MOF application resulted in significant enhancements in soil enzyme activities, with urease activity increasing by approximately 22.5% to 100.5%, peroxidase activity rising by around 24.2% to 148.5%, and invertase activity augmenting by about 5.4% to 32.9%. Notably, the M4 treatment in 2021 demonstrated a substantial jujube yield increase of approximately 19.22%, elevating from 7.65 t/ha to 9.12 t/ha. Based on comprehensive analysis, this study recommends an optimal MOF application rate of approximately 2.4 t/ha. This approach not only provides robust support for the sustainable development of the jujube industry but also serves as a valuable reference for enhancing local soil resilience against desertification. Full article

Creating wheat genotypes characterized by high grain yield, high protein content, and favorable amino acid composition is the main goal of breeders, especially in developing countries where wheat is a staple food. An experiment with 20 wheat genotypes, released through breeding activities in the Serbian region at different periods and adapted to its pedoclimatic conditions, was conducted with the aim of determining the genetic potential of the analyzed genotypes for grain yield and quality. Due to the divergence of the examined wheat germplasm, the factor of genotype had the largest share in the variation of all yield parameters (>66%). The genotypes Zadruga and Agrounija exhibited superior abilities for overall grain yield. Also, genotype Zadruga stood out in a distinct cluster group due to high values of both thousand grain weight and grain yield per plant. A continuous improvement in protein content was found, with newer genotypes having 17.13% higher protein content compared with older genotypes. Genotype Sloga stood out with the highest protein content (13.93%). On the other hand, the old genotype Balkan was distinguished by the highest content of nonessential amino acids (61.5 g 100 g⁻¹ protein), which makes it a good genetic resource. Genotypes Agrounija (32.62 g 100 g⁻¹ protein) and Tanjugovka (32.47 g 100 g⁻¹ protein) had the highest content of essential amino acids. The highest AAS value was established for tryptophan (1.81) and the lowest for lysine (0.61). Genotypes Tanjugovka and Zadruga had the highest AAS, i.e., protein completeness. The genotypes Zadruga, Tanjugovka, Agrounija, and Sloga have demonstrated high-yield capacity and possess a favorable amino acid profile, making them promising candidates for enhancing the nutritional quality of wheat and potentially benefiting human health. Full article

Fusarium wilt, caused by the fungus Fusarium oxysporum f. sp. vanillae (Fov), is a disease that results in significant losses in commercial vanilla production. The genera Ceratobasidium (Ceratobasidiaceae) and Tulasnella (Tulasnellaceae), which are often reported as mutualistic symbionts in orchids, belong to the form genus Rhizoctonia, a paraphyletic group of fungi with potential for pathogen biocontrol. We assayed the antagonistic properties of the form genus Rhizoctonia from the roots of neotropical orchids: two Tulasnella spp. isolates (Bv3 and Er1) and one Ceratobasidium sp. (Er19). In a dual culture, we found that form genus Rhizoctonia isolates can generate a biocontrol effect against Fusarium through the mechanisms of antibiosis and competition for space and nutrients. On histological observations, orchid root endophytes also demonstrated potential for mutualistic symbiosis development by establishing themselves on the surface and within the root tissue of Vanilla planifolia accessions multiplied in vitro (NSF021 and NSF092). However, in plant assays, the form genus Rhizoctonia isolates did not reduce symptom expression or disease development due to infection by Fov in the host. These results contribute to the knowledge of the interactions between tropical orchids and their microbiota and demonstrate the need for multidisciplinary studies for the implementation of integrated management strategies for Fusarium disease in commercial systems. Full article

In arid and semi-arid regions, rainfall takes on a critical significance to both agricultural and engineering construction activities, and the transport process and driving mechanism of soil water under rainfall conditions are in need of further investigation. To clarify the variations in soil moisture, temperature, and liquid and vapor flux under various rainfall scenarios, the Mu Us Sandy Land was selected as the study region, and a water–vapor–heat transport model was established using the Hydrus-1D software with in situ observed soil and meteorological data. The simulated results were in good agreement with the measured data during both the calibration and validation periods, suggesting that the model was accurate and applicable to the study region. The variations in the selected dry and rainy periods proved the significant effect of rainfall events on soil matric potential, temperature, and driving forces. When rainfall occurred, the hydraulic conductivity for liquid water rose by three to five orders of magnitude, driving the liquid water flow downward. In contrast, the vapor flux played a vital role in soil water movement, accounting for about 15% of the total water flux in the shallow layer when the soil was dry, while it became non-significant during rainy periods due to the reduction in hydraulic conductivity for vapor and the temperature gradient. These results clarified the mechanisms of soil liquid water and vapor movement in arid areas, which could provide scientific support for future studies on vegetation restoration and ecosystem sustainability in ecologically fragile areas. Full article

The addition of biochar-based organic fertilizer (BOF) can improve sugar beet yield, but its effects on the growth of sugar beet and on soil quality at different densities remain unclear. Six treatments, comprising two densities D1 and D2 (80,000 and 90,000 plant ha⁻¹) and three application rates B1, B2, and B3 (2.75, 3.25, and 3.75 t ha⁻¹) of BOFs + mineral fertilizer, respectively, are investigated in this research. The mineral fertilizers are typically used to supplement the total N, P₂O₅, and K₂O deficiencies. The BOFs were used in the soil before sowing, and the mineral fertilizer was added to the soil after the first pair of true leaves was grown. At 160 days after sowing (DAS), the root-to-shoot ratio under the D2B2 treatment was significantly higher than that under the other treatments. The effect of density on the photosynthesis rate of sugar beet was not significant. The BOF application amount and density exerted interaction effects on soil physicochemical properties and the activities of different soil enzymes affecting each other. Both the D2B2 and D2B3 treatments reduced the content of NO₃^–-N in the 40–60 cm soil layer. Combined 90,000 plants ha^–1 with 3.25 t ha⁻¹ BOFs can increase the soil nutrient content of the 0–60 cm soil layer, improve the rhizosphere soil environment, promote the uniform distribution of dry matter, and increase sugar production. Full article

Phenological shifts in peaches have been observed over the last few years due to the fluctuation of the seasonal climate conditions experienced during dormancy, affecting orchard management practices and influencing production and harvest dates. This study aimed to model the vegetative and floral budbreak of selected peach cultivars. Three peach cultivars, including “Rubyprince”, “Harvester”, and “Red Globe”, were considered in this study based on the representation of the early, early-mid, and mid-seasons. The prediction of the budbreak in peaches was assessed using different models that integrate the combination of chill and heat requirements. Models used include the Weinberger model, the modified Weinberger model, Utah, the dynamic model, and the growing degree model. The accumulation of chill varies according to the season evaluated. A model that considers both chill and heat accumulation is presented for each cultivar. Budbreak as an indicator of dormancy completion was established for each cultivar. The outcome of this study is to determine the amount of chilling accumulation and thermal time required to mark the beginning of the budbreak in selected cultivars with a model that predicts the duration of the dormancy. These results are valuable information that can be used for crop management practices and support the mitigation of cold damage during this critical period of crop development. Full article

With excessive nitrogen (N) input, high nitrous oxide (N₂O) emissions are frequently observed in greenhouse vegetable fields. We hypothesized that the underlying production mechanisms can be derived across a wide selection of vegetable fields in the middle and lower reaches of the Yangtze River. Thus, we investigated the emission characteristics and relative contributions of ammonia-oxidizing archaea (AOA) and bacteria (AOB) and other microbial processes to the N₂O production from five long-term greenhouse vegetable fields through an incubation experiment with combined inhibition methods. The results showed that the ammonia oxidation process is the dominant contributor to N₂O production at all five sites, accounting for 88–97% of the total N₂O emissions. Regardless of acidic, neutral, or alkaline soil, AOA-driven N₂O emission rates were consistently higher than AOB-driven N₂O emission rates. Both AOA-driven and AOB-driven N₂O emissions exhibited positive correlations with soil pH, with significant increases in soil N₂O production associated with high pH levels. Therefore, general production mechanisms were derived, such that more attention should be paid to AOA-driven N₂O emissions and to vegetable soils with a relatively high pH in the middle and lower reaches of the Yangtze River. Full article

Ratooning rice is an essential rice-planting method. However, mechanical harvesting of the primary rice crop, while increasing efficiency, can negatively affect the yield of ratooning rice. Therefore, it is crucial to find ways to improve the grain yield of ratooning rice after the mechanical harvest of the primary rice. A two-year field experiment was conducted; the grain yield of ratooning rice was assessed by stubble righting after mechanical harvesting of primary rice. The study used two popular rice cultivars, YLiangyou911 and Kenliangyou801, as experimental materials. The experimental treatments included three groups: one without righting after rolling rice stubble (CK), another with mechanized righting after rolling rice stubble (T1), and a third one without rolling rice stubble by the machine (T2). The results of the study demonstrate that stubble righting after the mechanical harvest of primary rice (T1) had a substantial impact on the grain yield of ratooning rice. It led to grain yields similar to ratooning rice without mechanical rolling of the rice stubble (T2) and significantly outperformed the treatment without stubble righting after the mechanical harvest of primary rice (CK). The study observed significant effects of the year of the experiment (Y), the treatment applied (T), and the interaction between year and treatment (Y×T) on grain yield. Additionally, the treatment showed a significant influence on the yield components. Specifically, in 2021, the T1 and T2 treatments showed remarkable grain yield increases in YLiangyou911 by 107.41% and 147.97%, respectively, compared to CK. For Kenliangyou801 in 2021, the T1 and T2 treatments also resulted in notable improvements in grain yield by 45.85% and 114.26%, respectively. Similarly, in 2022, the grain yield increased by 6.99% for T1 and 23.87% for T2 in YLiangyou911, and by 77.23% for T1 and 187.13% for T2 in Kenliangyou801, compared to CK. The study also detected enhancements in several aspects, including biomass accumulation, solar radiation and photosynthetic characteristics, antioxidant response and nitrogen metabolism, and bud-regeneration capacity due to T1 and T2 treatments. Furthermore, correlation analysis was conducted to assess the relationship between grain yield and the investigated parameters. In conclusion, stubble righting after the mechanical harvest of primary rice resulted in significantly improved grain yield for ratooning rice. This improvement can be attributed to enhanced biomass accumulation, solar radiation and photosynthetic characteristics, antioxidant response and nitrogen metabolism, and increased bud-regeneration capacity. Full article

To detect quickly and accurately “Yuluxiang” pear fruits in non-structural environments, a lightweight YOLO-GEW detection model is proposed to address issues such as similar fruit color to leaves, fruit bagging, and complex environments. This model improves upon YOLOv8s by using GhostNet as its backbone for extracting features of the “Yuluxiang” pears. Additionally, an EMA attention mechanism was added before fusing each feature in the neck section to make the model focus more on the target information of “Yuluxiang” pear fruits, thereby improving target recognition ability and localization accuracy. Furthermore, the CIoU Loss was replaced with the WIoUv3 Loss as the loss function, which enhances the capability of bounding box fitting and improves model performance without increasing its size. Experimental results demonstrated that the enhanced YOLO-GEW achieves an F1 score of 84.47% and an AP of 88.83%, while only occupying 65.50% of the size of YOLOv8s. Compared to lightweight algorithms such as YOLOv8s, YOLOv7-Tiny, YOLOv6s, YOLOv5s, YOLOv4-Tiny, and YOLOv3-Tiny; there are improvements in AP by 2.32%, 1.51%, 2.95%, 2.06%, 2.92%, and 5.38% respectively. This improved model can efficiently detect “Yuluxiang” pears in non-structural environments in real-time and provides a theoretical basis for recognition systems used by picking robots. Full article

It is intriguing to understand the influence of the watering volume and growing design on the growth, yield, and quality of cherry tomatoes. This study sought to identify the most effective watering volume and growing design treatment for the production and quality of the cherry tomato cultivar Ruby. This study was an exploration using a factorial experiment with an RCBD design. The treatment factors included the growing design (greenhouse, rain shelter, and screen house) and the watering volume (100% ETc, 75% ETc, and 50% ETc). The results showed that the root dry weight and root–shoot ratio were higher in the screen house design, while the fruit firmness was higher in the rain shelter design. Considering the effect of the watering volume, a higher fruit diameter, heavier fruits, more fruits per plant, higher ultimate fruit and biological yield per plant, higher root and shoot biomass, better root–shoot ratio, higher leaf chlorophyll content, greater fruit skin firmness, and greater elasticity were noted for the 100% ETc treatment. In contrast, the growth rate was higher for the 50% ETc treatment. Cultivating cherry tomatoes in a greenhouse using a watering volume of 100% ETc is recommended based on the results. These conditions led to better growth, higher fruit yield, and improved fruit quality, making them favorable options for successful cherry tomato production. Full article

Throughout the world, salinity is a major environmental issue that limits agricultural productivity, particularly in arid and semi-arid regions. In addition, climate change is the most important reason for the salinization of agricultural soils in the world, so it is now essential to find solutions to increase salinity tolerance in plants. This study investigated the potential of arbuscular mycorrhizal fungi (AMF) inoculation to enhance the growth and yield performances of flax under different salinity levels by conducting a pot experiment. The experiment was laid out in a two-factor completely randomized design including AMF inoculation (AMF+: with inoculation; AMF−: without inoculation) and irrigation water salinity (0, 50, 100, and 150 mM NaCl). According to the results, it is evident that salt stress caused negative physiological effects, including limited growth, reduced photosynthesis, and decreased nitrogen (N) and phosphorus (P) content in the shoots and roots of flax plants. Moreover, mycorrhizal association improved the salt tolerance of the plants by increasing chlorophyll content, and enhancing N and P shoot and root contents and consequently yield parameters, such as seed and stem fiber yield, particularly at moderate salt concentrations (50 and 100 mM NaCl). In particular, under 100 mM, AMF increased the total chlorophyll content, N shoot and root content, P shoot and root content, and seed and stem fiber yield by 30.4%, 36.1%, 31.0%, 38.9%, 45.4%, 35.2%, and 26.9%, respectively. As a result of using AMF, flax plants grown under salt stress exhibited tolerance, suggesting that AMF could be applied in saline environments to maintain ecological stability. Full article

Fall armyworm (FAW), Spodoptera frugiperda, invaded the south of China in December 2018 and has since posed a huge threat to crop production in China. However, transgenic Bacillus thuringiensis (Bt) corn can efficiently control the damage caused by FAWs. In fact, the Chinese government has issued biosafety certificates for several Bt corn hybrids expressing any one of four Bt proteins, Vip3A, Cry1F, Cry1Ab, and Cry2Ab, or combinations thereof, to control FAWs. These Bt corn events are soon to be commercialized in China. Therefore, it is necessary to monitor and evaluate whether the FAW has developed resistance to any of the Bt corn hybrids planted in fields in China. To address this issue, we collected 11 geographical populations of FAWs and determined the sensitivity of each to the aforementioned four purified Bt proteins as assessed by diet surface overlay bioassays. The ranges for the 50% lethal concentration (LC₅₀) of the four Bt proteins to all FAW populations were as follows: 11.42–88.33 ng/cm² (for Vip3A), 111.21–517.33 (Cry1F), 135.76–1108.47 (Cry1Ab), and 994.42–5492.50 (Cry2Ab). The corresponding ranges for the 50% growth inhibition concentrations (GIC₅₀) were 1.43–14.86, 2.35–138.97, 1.58–464.86, and 25.01–1266.07 ng/cm². The lethal effects and growth inhibition effects of the four Bt proteins on FAW were in the same order of Vip3A > Cry1F > Cry1Ab > Cry2Ab. A comparison with published LC₅₀ values of Bt proteins towards sensitive FAW populations revealed that all 11 FAW populations in this study were sensitive to Vip3A, Cry1F, and Cry1Ab. This study provides foundational data for monitoring and controlling the resistance of Bt corn to FAW in China. Full article

This paper highlights the molecular composition of native peat and preparations of humic substances (HSs) isolated from permafrost hummock peatlands (Histosols) of the forest tundra zone of the European north-east of Russia. The structural and functional parameters of humic—(HAs) and fulvic acids (FAs) of the peatlands studied are determined by the combined action of cryogenic processes, species composition and the degree of peat decomposition, which reflects the climatic conditions during peat formation in the Holocene. The predominance of the proportion of HAs over FAs in the composition of peat, as well as the low acidity of FAs, makes HS-based preparations highly promising for use as organic fertilizers. The high contents of alkyl and carbohydrate fragments in the structure of the studied HSs allow us to recommend them for use in mineral loamy-textured soils. Full article