Systematic Analysis and Expression Profiling of the Ginger FWL Gene Family Reveal Its Potential Functions in Rhizome Development and Response to Abiotic Stress
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material Growth and Treatment Conditions
2.2. Identification and Physicochemical Properties Analysis of Ginger FWL Gene-Family Members
2.3. Phylogenetic Analysis of ZoFWL Gene Family
2.4. Analysis of Factorial Structure, Conserved Motifs, and Cis-Acting Elements
2.5. Gene Replication, Collinearity Analysis, and Protein Interaction Network Prediction
2.6. Expression Pattern and qRT-PCR Analysis of ZoFWL
2.7. Statistical Analyses
3. Results
3.1. Identification and Sequence Analysis of the Ginger FWL Gene Family
3.2. Chromosome Localization Analysis of ZoFWL Genes
3.3. Phylogenetic Analysis of the ZoFWL Gene Family
3.4. Phylogenetic Evolution, Gene Structure, and Conserved Motif Analysis of ZoFWL Gene
3.5. Analysis of Cis-Acting Elements of the ZoFWL Gene Family
3.6. Genome-Wide Duplication Events and Collinearity Analysis of ZoFWLs
3.7. Expression Patterns of ZoFWL Genes in Different Ginger Organs
3.8. Expression Patterns of ZoFWL Genes under Abiotic Stress
3.9. Prediction of ZoFWL Protein Interaction Network
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene Name | Gene ID | Chromosome | Localization (bp) | Animo Acid Number | Molecular Weight (kDa) | PI | Subcellular Localization |
---|---|---|---|---|---|---|---|
ZoFWL1 | Maker00025051 | Chr06 | 70661613–70663046(+) | 477 | 53.43 | 8.46 | Cell membrane Nucleus |
ZoFWL2 | Maker00026518 | Chr06 | 107790889–107801973(+) | 239 | 26.76 | 5.03 | Cell membrane Chloroplast |
ZoFWL3 | Maker00016179 | Chr08 | 118773164–118778419(−) | 551 | 62.51 | 8.02 | Cell membrane |
ZoFWL4 | Maker00009473 | Chr10 | 15788317–15794441(+) | 239 | 26.58 | 4.88 | Cell membrane |
ZoFWL5 | Maker00032137 | Chr10 | 133341584–133342428(−) | 123 | 13.24 | 4.46 | Cell membrane |
ZoFWL6 | Maker00032080 | Chr10 | 133355752–133356594(+) | 203 | 21.90 | 6.34 | Cell membrane |
ZoFWL7 | Maker00032144 | Chr10 | 133496230–133497188(+) | 204 | 22.30 | 5.69 | Cell membrane |
ZoFWL8 | Maker00030347 | Chr10 | 139765351–139766271(−) | 204 | 22.36 | 5.87 | Cell membrane |
ZoFWL9 | Maker00030271 | Chr10 | 139875528–139876205(−) | 167 | 18.08 | 6.37 | Cell membrane |
ZoFWL10 | Maker00020626 | Chr12 | 3849932–3854545(−) | 357 | 40.03 | 6.92 | Cell membrane Nucleus |
ZoFWL11 | Maker00042686 | Chr12 | 14606275–14607401(+) | 217 | 23.71 | 4.87 | Cell membrane |
ZoFWL12 | Maker00035127 | Chr14 | 122859462–122864489(−) | 273 | 30.00 | 4.93 | Cell membrane |
ZoFWL13 | Maker00014483 | Chr14 | 135425628–135429693(−) | 277 | 30.37 | 5.43 | Cell membrane |
ZoFWL14 | Maker00004172 | Chr16 | 2000297–2001809(+) | 369 | 40.34 | 4.92 | Cell membrane Nucleus |
ZoFWL15 | Maker00009037 | Chr16 | 113827519–113842946(−) | 737 | 83.68 | 6.11 | Cell membrane Nucleus |
ZoFWL16 | Maker00012794 | Chr18 | 40089638–40092331(+) | 244 | 26.93 | 5.36 | Cell membrane |
ZoFWL17 | Maker00028616 | Chr18 | 82462280–82465007(+) | 228 | 25.25 | 4.52 | Cell membrane |
ZoFWL18 | Maker00022350 | Chr20 | 4671208–4672823(−) | 182 | 19.83 | 6.83 | Cell membrane |
ZoFWL19 | Maker00022475 | Chr20 | 4717902–4718711(−) | 170 | 18.76 | 6.75 | Cell membrane |
ZoFWL20 | Maker00026904 | Chr20 | 24062166–24063058(+) | 176 | 19.06 | 7.47 | Cell membrane |
ZoFWL21 | Maker00001158 | Chr22 | 173270323–173283255(+) | 222 | 24.11 | 4.97 | Cell membrane |
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Jiang, Y.; Tang, S.; Xia, M.; Li, H.; Xiao, D.; Li, X.; Xing, H.; Wang, B.; Huang, H.; Zhou, S.; et al. Systematic Analysis and Expression Profiling of the Ginger FWL Gene Family Reveal Its Potential Functions in Rhizome Development and Response to Abiotic Stress. Agronomy 2024, 14, 1805. https://doi.org/10.3390/agronomy14081805
Jiang Y, Tang S, Xia M, Li H, Xiao D, Li X, Xing H, Wang B, Huang H, Zhou S, et al. Systematic Analysis and Expression Profiling of the Ginger FWL Gene Family Reveal Its Potential Functions in Rhizome Development and Response to Abiotic Stress. Agronomy. 2024; 14(8):1805. https://doi.org/10.3390/agronomy14081805
Chicago/Turabian StyleJiang, Yajun, Shihao Tang, Maoqin Xia, Hui Li, Daoyan Xiao, Xingyue Li, Haitao Xing, Biao Wang, Hao Huang, Shengmao Zhou, and et al. 2024. "Systematic Analysis and Expression Profiling of the Ginger FWL Gene Family Reveal Its Potential Functions in Rhizome Development and Response to Abiotic Stress" Agronomy 14, no. 8: 1805. https://doi.org/10.3390/agronomy14081805