microRNA Expression Dynamics in Culicoides sonorensis Biting Midges Following Blood-Feeding
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Culicoides sonorensis Maintenance and Blood Feeding
2.2. Sample Collection
2.2.1. Insect Sample Collection
2.2.2. Cell Line Sample Collection
2.3. RNA Isolation
2.4. Sequencing
2.5. Bioinformatic Analyses
2.5.1. miRNA Identification
2.5.2. Identification of Conserved miRNAs
2.5.3. Differential Expression Analyses
2.5.4. Identification of miRNAs Potentially Involved in Viral Infection
2.6. RT-qPCR Analyses
3. Results
3.1. Small RNA Sequencing Libraries
3.2. Annotated miRNAs
3.3. Transcriptome Analyses across Blood Meal and Tissue
3.4. miRNAs Potentially Involved in Culicoides–Virus Interactions
4. Discussion
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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miRNA | Tissue Enrichment | BM—Expression * |
---|---|---|
cso-miR-100 | MG | |
cso-miR-1174 | MG | |
cso-miR-1175 | MG | |
cso-miR-12 | MG | |
cso-miR-190 | MG | + |
cso-miR-2796 | MG | − |
cso-miR-281 | MG | |
cso-miR-283 | MG | |
cso-miR-2942 | MG | |
cso-miR-305 | MG | |
cso-miR-306 | MG | |
cso-miR-308 | MG | |
cso-miR-316 | MG | |
cso-miR-33 | MG | |
cso-miR-956 | MG | |
cso-miR-965 | MG | |
cso-miR-988 | MG | |
cso-miR-9a | MG | |
cso-miR-9b | MG | |
cso-miR-1 | n-MG | |
cso-miR-1000 | n-MG | |
cso-miR-124 | n-MG | |
cso-miR-133 | n-MG | + |
cso-miR-137 | n-MG | |
cso-miR-210 | n-MG | |
cso-miR-252 | n-MG | |
cso-miR-263a | n-MG | + |
cso-miR-263b | n-MG | + |
cso-miR-277 | n-MG | |
cso-miR-285 | n-MG | + |
cso-miR-286a | n-MG | + |
cso-miR-2944a | n-MG | + |
cso-miR-310 | n-MG | |
cso-miR-315 | n-MG | + |
cso-miR-7 | n-MG | |
cso-miR-87 | n-MG | |
cso-miR-927 | n-MG | |
cso-miR-929 | n-MG | |
cso-miR-932 | n-MG | + |
cso-miR-957 | n-MG | + |
cso-miR-981 | n-MG | + |
cso-miR-993 | n-MG | |
cso-miR-X3 | n-MG |
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Mills, M.K.; Rozo-Lopez, P.; Bryant, W.B.; Drolet, B.S. microRNA Expression Dynamics in Culicoides sonorensis Biting Midges Following Blood-Feeding. Insects 2023, 14, 611. https://doi.org/10.3390/insects14070611
Mills MK, Rozo-Lopez P, Bryant WB, Drolet BS. microRNA Expression Dynamics in Culicoides sonorensis Biting Midges Following Blood-Feeding. Insects. 2023; 14(7):611. https://doi.org/10.3390/insects14070611
Chicago/Turabian StyleMills, Mary Katherine, Paula Rozo-Lopez, William Bart Bryant, and Barbara S. Drolet. 2023. "microRNA Expression Dynamics in Culicoides sonorensis Biting Midges Following Blood-Feeding" Insects 14, no. 7: 611. https://doi.org/10.3390/insects14070611