AoRan1 Is Involved in Regulating Conidiation, Stress Resistance, Secondary Metabolism, and Pathogenicity in Arthrobotrys oligospora
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains and Culture Conditions
2.2. Phylogenetic Analysis of AoRan1
2.3. Deletion of Aoran1
2.4. Comparison of Hyphal Growth and Sporulation
2.5. Comparison of Mycelial Growth under Different External Pressures
2.6. Comparison of Pathogenicity and Observation of Trap Morphology
2.7. Staining Analysis
2.8. RT-qPCR Analysis
2.9. Metabolomics Analysis
2.10. Statistical Analysis
3. Results
3.1. Sequence Analysis of AoRan1
3.2. Verification of Positive Transformants
3.3. Comparison of Growth Rates between WT and ∆Aoran1 Mutant Strains
3.4. AoRan1 Plays a Vital Role in Conidiation
3.5. AoRan1 Is Critical to Trap Formation and Pathogenicity
3.6. AoRan1 Is Involved in Stress Response
3.7. AoRan1 Regulates LD Accumulation and Autophagic Level
3.8. AoRan1 Contributes to Secondary Metabolism
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Duan, S.; Liu, Q.; Shen, Y.; Zhu, L.; Yuan, H.; Yang, J. AoRan1 Is Involved in Regulating Conidiation, Stress Resistance, Secondary Metabolism, and Pathogenicity in Arthrobotrys oligospora. Microorganisms 2024, 12, 1853. https://doi.org/10.3390/microorganisms12091853
Duan S, Liu Q, Shen Y, Zhu L, Yuan H, Yang J. AoRan1 Is Involved in Regulating Conidiation, Stress Resistance, Secondary Metabolism, and Pathogenicity in Arthrobotrys oligospora. Microorganisms. 2024; 12(9):1853. https://doi.org/10.3390/microorganisms12091853
Chicago/Turabian StyleDuan, Shipeng, Qianqian Liu, Yanmei Shen, Lirong Zhu, Hui Yuan, and Jinkui Yang. 2024. "AoRan1 Is Involved in Regulating Conidiation, Stress Resistance, Secondary Metabolism, and Pathogenicity in Arthrobotrys oligospora" Microorganisms 12, no. 9: 1853. https://doi.org/10.3390/microorganisms12091853