Microbial Mediated Synthesis of Silver Nanoparticles by Lactobacillus Plantarum TA4 and its Antibacterial and Antioxidant Activity
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Bacterial Culture and Biomass Extraction
2.3. Determination of Maximum Tolerable Concentration (MTC) against Ag+
2.4. Biosynthesis of Silver Nanoparticles (AgNPs) Using L. plantarum TA4
2.5. Characterization of Biosynthesized AgNPs
2.6. Scanning Electron Microscope (SEM) and Energy Dispersive X-ray (EDX) of L. plantarum TA4 Exposed to Ag+
2.7. Antibacterial Assay of Biosynthesized AgNPs
2.8. Antioxidant Activity of Biosynthesized AgNPs
2.9. Statistical Analysis
3. Results and Discussion
3.1. Maximum Tolerable Concentration (MTC) of L. plantarum TA4 against Ag+
3.2. Biosynthesis of AgNPs Using L. plantarum TA4 Biomass
3.3. Dynamic Light Scattering (DLS) Analysis
3.4. FTIR Analysis
3.5. SEM-EDX and HR-TEM Analysis
3.6. Antibacterial Activity of Biosynthesized AgNPs
3.7. Antioxidant Activity of Biosynthesized AgNPs
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Species | Route | Size (nm) | Reference |
---|---|---|---|
Lactobacillus sp. | Supernatant | 30–100 | [19] |
Lactobacillus acidophilus | Cell biomass | 30 | [35] |
Lactobacillus plantarum | Cell biomass | 33.4 | [35] |
L. acidophilus | Supernatant | 4–50 | [17] |
Lactobacillus delbrueckii | Supernatant | 54–113 | [36] |
Lactobacillus sp. | Supernatant | 14 | [37] |
Lactobacillus mindensis | Supernatant | 20 | [38] |
Spectra Band (cm−1) | Functional Group and Vibrational Assignment | |
---|---|---|
L. plantarum TA4 | Biosynthesized AgNPs | |
3254 | 3197 | Hydroxyl group (-OH)/amide group (N–H stretching vibration) |
2924 | C–H stretching vibration | |
1636 | 1634 | Amide I (C=O stretching vibration) |
1151 | 1518 | Amide II |
1456 | 1450 | C–H bending vibration |
1224 | 1209 | C–N stretching vibration, PO2- asymmetric stretching |
1078 | 1030 | C–O stretching vibration |
994 | 961 | C=C bending vibration |
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Mohd Yusof, H.; Abdul Rahman, N.; Mohamad, R.; Zaidan, U.H. Microbial Mediated Synthesis of Silver Nanoparticles by Lactobacillus Plantarum TA4 and its Antibacterial and Antioxidant Activity. Appl. Sci. 2020, 10, 6973. https://doi.org/10.3390/app10196973
Mohd Yusof H, Abdul Rahman N, Mohamad R, Zaidan UH. Microbial Mediated Synthesis of Silver Nanoparticles by Lactobacillus Plantarum TA4 and its Antibacterial and Antioxidant Activity. Applied Sciences. 2020; 10(19):6973. https://doi.org/10.3390/app10196973
Chicago/Turabian StyleMohd Yusof, Hidayat, Nor’Aini Abdul Rahman, Rosfarizan Mohamad, and Uswatun Hasanah Zaidan. 2020. "Microbial Mediated Synthesis of Silver Nanoparticles by Lactobacillus Plantarum TA4 and its Antibacterial and Antioxidant Activity" Applied Sciences 10, no. 19: 6973. https://doi.org/10.3390/app10196973
APA StyleMohd Yusof, H., Abdul Rahman, N., Mohamad, R., & Zaidan, U. H. (2020). Microbial Mediated Synthesis of Silver Nanoparticles by Lactobacillus Plantarum TA4 and its Antibacterial and Antioxidant Activity. Applied Sciences, 10(19), 6973. https://doi.org/10.3390/app10196973