Global Phenolic Composition and Antioxidant Capacity of Extracts from the Endophytic Fungi Cophinforma mamane with Potential Use in Food Systems: The Effects of Time, Temperature, and Solvent on the Extraction Process

Round 1

Reviewer 1 Report

Comments and Suggestions for Authors

This article entitled "Global phenolic composition and antioxidant capacity of extracts from the endophytic fungi Cophinforma mamane with potential use in food systems: effect of time, temperature and solvent on the extraction process", the authors that the best extraction condition was defined by evaluating the binomial “total phenolic compounds antioxidant activity” obtained with 2 h of extraction with AcOEt at 20 ℃.

It is significant value in assessing the potential utilization of phenolic extracts derived from endophytic fungi as additives or adjuvants in the food industry, therefore these results are potential of practical use. Overall, the manuscript is properly organized and nearly written. I suggest to accept the article for publication after a minor revision. The authors need to address the comments to improve the quality of the manuscript significantly.

1.       How about the screening of fungus C. mamane CF2-13 isolated from F. chica ?

2.       Are there any other substances, except polyphenols, for example, alkaloids and peptides in extracts. Whether these substances affect the antioxidant capacity or not?

3.       There were already similar studies on that research field. Authors can clearly give statements on novelty, necessity, and justification in Conclusions Section.

4.       Please recheck the statistical analysis in Figure 1A, Figure 2A, etc.

5.       Please recheck the writing format, for example, “h” or “hour” in line 512, etc.

Comments on the Quality of English Language

Please recheck the writing format, for example, “h” or “hour”, etc.

Author Response

Please, see the attachment containing the response and comments to reviewer 1.

Author Response File: Author Response.pdf

Reviewer 2 Report

Comments and Suggestions for Authors

General comments

Comments 1: The selection of the extraction method is appropriate, given its wide application in food industry to produce extracts. However, the choice of solvents such as ethyl acetate, 1-butanol (ButOH), AcOEt, etc., must be carefully justified, taking into account that the extract is intended for use in the food industry. 

Comments 2: The authors mention that they optimised the extraction process and the overall amount of phenolic compounds, how was this done? Was an optimisation model or experimental design used? What is your criterion to affirm that the extraction method was optimised?

Comments 3: The amount of total phenols in the extract and the antioxidant capacity is not high compared to other sources of bioactive compounds (fruits), so why state that there is a high amount of these bioactive compounds in the extracts obtained? 

 

Author Response

Please, see the attachment containing the response and comments to reviewer 2.

Author Response File: Author Response.pdf

Reviewer 3 Report

Comments and Suggestions for Authors

The manuscript on “Global phenolic composition and antioxidant capacity of extracts from the endophytic fungus Cophinforma mamane with potential use in food systems: effect of time, temperature and solvent on the extraction process” is an experimental article, clearly and well-structured. The authors have isolated endophytic mushrooms Conphinforma mamane, extracted their metabolites, and studied the antioxidant activity of their extracts. The optimal conditions for the extraction of endophytic fungi were established. It was determined that the mushroom extract was able to protect olive oil against lipid oxidation, without a significant difference compared to tocopherol, indicating its potential as an antioxidant for use in the food industry.

1. In subsection 2.1, the purity of the reagents used and where they were used should be indicated.

2. Describe the method of isolation of endophytic fungi Conphinforma mamane CF2-13.

 

This manuscript is scientifically substantiated, is characterized by relevance and novelty, and contains 45 references. The conclusions are consistent with the evidence and arguments presented. Ethical and data availability statements are adequate.

In my opinion, the manuscript can be accepted for publication in the journal Applied Sciences after minor revisions.

Comments for author File: Comments.pdf

Comments on the Quality of English Language

+

Author Response

Please, see the attachment containing the response and comments to reviewer 3.

Author Response File: Author Response.pdf

Round 2

Reviewer 2 Report

Comments and Suggestions for Authors

The authors have corrected the remarks