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Journal of Functional Biomaterials, Volume 16, Issue 10

2025 October - 38 articles

Cover Story: Bone regeneration relies on the balance between osteoblast-mediated bone formation and osteoclast-driven resorption, governed by complex biochemical cues. Magnesium ions (Mg2+) critically regulate these processes, yet their dual influence on osteogenesis and osteoclastogenesis remains unclear. In this study, Mg2+ exhibited dose-dependent effects in 2D culture, with moderate concentrations suppressing osteoclast maturation while limiting osteogenic differentiation. Incorporating Mg2+ into collagen/nano-hydroxyapatite (Coll/nHA) scaffolds enabled localized delivery, revealing synergistic Mg2+–nHA interactions that enhanced bone matrix deposition and reduced osteoclast activity, offering a promising strategy for advanced bone tissue engineering. View this paper
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Articles (38)

  • Article
  • Open Access
2,042 Views
20 Pages

Experimental Biomechanical Analysis of the Bone-to-Implant Connection in Single-Piece Implants

  • Karina Krawiec,
  • Adam Kurzawa,
  • Jakub J. Słowiński,
  • Calin Romulus Fodor and
  • Łukasz Pałka

19 October 2025

The mechanical properties of dental implants are critical for their durability. The purpose of this study was to determine the maximum force required to induce full pull-out of a titanium implant from the bone and to characterize the mechanical behav...

  • Article
  • Open Access
1 Citations
3,433 Views
19 Pages

Biological Characterization of 3D-Printed, Sintered Hydroxyapatite Scaffolds Obtained by Fused Filament Fabrication: An In Vitro Study

  • Eddy Shan,
  • Cristina Chamorro,
  • Ana Ferrández-Montero,
  • Rosa M. Martin-Rodriguez,
  • Leire Virto,
  • María José Marín,
  • Begoña Ferrari,
  • Antonio Javier Sanchez-Herencia,
  • Elena Figuero and
  • Mariano Sanz

19 October 2025

This study characterized the biological response of MG-63 cells to synthetic, hydroxyapatite scaffolds (HAsint) fabricated via fused filament fabrication. Scaffolds were compared to 2D plate-adherent cultures using six assays: cell morphology and dis...

  • Article
  • Open Access
1 Citations
1,284 Views
20 Pages

Strength–Ductility Synergy in Biodegradable Mg-Rare Earth Alloy Processed via Multi-Directional Forging

  • Faseeulla Khan Mohammad,
  • Uzwalkiran Rokkala,
  • Sohail M. A. K. Mohammed,
  • Hussain Altammar,
  • Syed Quadir Moinuddin and
  • Raffi Mohammed

18 October 2025

In this study, a biodegradable Mg-Zn-Nd-Gd alloy was processed via multi-directional forging (MDF) to evaluate its microstructural evolution, mechanical performance, and corrosion behavior. Electron backscattered diffraction (EBSD) analysis was condu...

  • Article
  • Open Access
2,271 Views
25 Pages

Developments and Assessments of Crude Tea Saponin-Incorporated Silica Nanoparticles for Their Bioactivity Improvement

  • Tanrada Likitsatian,
  • Pimpisid Koonyosying,
  • Sittiruk Roytrakul,
  • Patcharawan Srisilapanan,
  • Somdet Srichairatanakool and
  • Jetsada Ruangsuriya

17 October 2025

The use of saponins with biosurfactant, antioxidant, anti-inflammatory, and anti-cancer properties is limited by their toxicity and bioavailability. This study focused on the fabrication, characterization, and bioactivity of crude tea saponin (TS) an...

  • Article
  • Open Access
2 Citations
1,732 Views
14 Pages

Plate Breakage After Mandibular Condylar Fracture Osteosynthesis

  • Marcin Kozakiewicz,
  • Paulina Agier and
  • Paulina Pruszyńska

16 October 2025

Despite the significant ongoing development of fixation materials, plate breakages still occur after osteosynthesis of the mandibular condyles. The aim of this study is to demonstrate the complications caused by fixation material breakages in the hop...

  • Article
  • Open Access
1,033 Views
14 Pages

Antibacterial PEEK-Ag Surfaces: Development and In Vitro Evaluation Against Staphylococcus aureus and Pseudomonas aeruginosa

  • Flávio Rodrigues,
  • Mariana Fernandes,
  • Filipe Samuel Silva,
  • Óscar Carvalho and
  • Sara Madeira

16 October 2025

In the pursuit of addressing the persistent challenge of bacterial adhesion and biofilm formation in dental care, this study investigates the efficacy of electric current as an alternative strategy, specifically focusing on its application in dental...

  • Article
  • Open Access
2 Citations
1,317 Views
13 Pages

15 October 2025

This randomized, prospective clinical trial was conducted to compare the effectiveness of the oscillating chitosan brush (OCB) and an air-abrasive device (AAD) in improving clinical outcomes during non-surgical management of peri-implant mucositis. F...

  • Article
  • Open Access
1,223 Views
16 Pages

Fibronectin- and Bioactive Glass-Modified Alginate Scaffolds Support Limited Primary Cell Proliferation In Vitro yet Demonstrate Effective Host Integration In Vivo

  • Benedetta Guagnini,
  • Andrea Mazzoleni,
  • Adrien Moya,
  • Arnaud Scherberich,
  • Barbara Medagli,
  • Ivan Martin,
  • Davide Porrelli,
  • Manuele G. Muraro and
  • Gianluca Turco

15 October 2025

Alginate-hydroxyapatite (AL) scaffolds modified with fibronectin (FN) or bioactive glass (BGMS10) have recently been characterized for their physicochemical properties and proposed as promising candidates for bone regeneration. Here, we present their...

  • Review
  • Open Access
1,565 Views
17 Pages

14 October 2025

Impaired bone regeneration and wound healing represent a major clinical and socioeconomic challenge for our aging and multimorbid population. Fracture and wound healing share many common features, with transforming growth factor beta (TGF-β) bei...

  • Review
  • Open Access
1 Citations
1,926 Views
20 Pages

Natural Polymers, Their Modifications and Composites with Synthetic Polymers for Tympanic Membrane Regeneration

  • Roman O. Shaikenov,
  • Polina G. Serbun,
  • Jingran Zhang,
  • Hao Wu,
  • Zuobin Wang,
  • Petr P. Snetkov and
  • Svetlana N. Morozkina

13 October 2025

The perforation of the tympanic membrane is a world-wide clinical problem resulting from trauma or infection and requiring effective regeneration methods. In recent years researchers have focused on natural polymers as promising materials for restori...

  • Review
  • Open Access
7 Citations
3,977 Views
29 Pages

12 October 2025

Decellularized extracellular matrix (dECM) scaffolds preserve native tissue structure and biochemical cues while minimizing immune responses, creating biomimetic templates that promote cell integration and tissue remodeling. This review examines the...

  • Article
  • Open Access
1 Citations
1,403 Views
17 Pages

11 October 2025

Open reduction and internal fixation (ORIF) for mandibular condyle fractures remains a controversial and challenging issue, with the exception of basal and low-neck fractures. Currently, there is a consensus that fractures causing irreparable maloccl...

  • Review
  • Open Access
1 Citations
3,232 Views
30 Pages

10 October 2025

Local recurrence after breast cancer surgery presents a critical challenge, demanding novel local immunotherapies capable of eliminating residual disease while avoiding systemic toxicity. In situ-forming hydrogels, functionalized with bioactive cargo...

  • Article
  • Open Access
1 Citations
1,649 Views
19 Pages

Improving the Biocompatibility of Plant-Derived Scaffolds for Tissue Engineering Using Heat Treatment

  • Arvind Ramsamooj,
  • Nicole Gorbenko,
  • Cristian Olivares,
  • Sashane John and
  • Nick Merna

10 October 2025

Small-diameter vascular grafts often fail due to thrombosis and compliance mismatch. Decellularized plant scaffolds are a biocompatible, sustainable alternative. Leatherleaf viburnum leaves provide natural architecture and mechanical integrity suitab...

  • Article
  • Open Access
1,195 Views
20 Pages

10 October 2025

Suture anchors are widely used for tendon and ligament repair, but their fixation strength is compromised in osteoporotic bone and limited bone volume such as the coracoid process. Existing designs are prone to penetration and insufficient cortical e...

  • Article
  • Open Access
1,766 Views
16 Pages

A Preliminary Randomized Trial on the Efficiency and Clinical Value of a Cementless Screw-Retained Implant Workflow in Single-Implant Restorations

  • Sang-Yoon Park,
  • Sung-Woon On,
  • Tae-Yoon Park,
  • Seoung-Won Cho,
  • Sang-Min Yi,
  • Soo-Hwan Byun,
  • Hyun-Sook Han,
  • Lee-Kyoung Kim and
  • Byoung-Eun Yang

10 October 2025

This randomized controlled clinical trial compared a conventional combined screw- and cement-retained prosthesis (CSCRP) workflow (control group) with a fully digital cementless screw-retained prosthesis (CL-SRP) system (test group) for single poster...

  • Article
  • Open Access
1,663 Views
18 Pages

Collagen Analogs Promote Tissue Regeneration in HSV-1-Infected Corneas in Animal Models

  • Oleksiy Buznyk,
  • Hamid Goodarzi,
  • Jaime Gómez Laguna,
  • Jaganmohan Reddy,
  • Aneta Liszka,
  • Elle Edin,
  • Christos Boutopoulos,
  • James Chodosh,
  • Mohammad Mirazul Islam and
  • May Griffith

Herpes simplex virus type 1 (HSV-1) is a leading cause of infectious corneal blindness worldwide. Human donor corneal transplantation remains the primary treatment for scarred corneas resulting from herpes simplex keratitis (HSK), a severe inflammato...

  • Article
  • Open Access
3,903 Views
13 Pages

Background: Pulp canal calcification (PCC) poses a challenge for endodontic treatment, as it obscures the canal and increases the risk of complications. This study aimed to evaluate the accuracy of endodontic access cavity preparation using dynamic n...

  • Article
  • Open Access
1,246 Views
14 Pages

Fracture Resistance of 3D-Printed Hybrid Abutment Crowns Made from a Tooth-Colored Ceramic Filled Hybrid Composite: A Pilot Study

  • Josef Schweiger,
  • Kurt-Jürgen Erdelt,
  • Isabel Lente,
  • Daniel Edelhoff,
  • Tobias Graf and
  • Oliver Schubert

The aim of this pilot in vitro study is to investigate the fracture strength of hybrid abutment crowns (HACs) made of a 3D-printable, tooth-colored, ceramic-reinforced composite (CRC). Based on an upper first premolar, a crown was designed, and speci...

  • Article
  • Open Access
1,124 Views
14 Pages

Long-Term Chemical Solubility of 2.3Y-TZP Dental Ceramics

  • Lidija Ćurković,
  • Sanja Štefančić,
  • Irena Žmak,
  • Vilko Mandić,
  • Ivana Gabelica and
  • Ketij Mehulić

In this study, the chemical solubility (stability) of yttria-partially stabilized zirconia (2.3Y-TZP) dental ceramics, both glazed (Group 2) and non-glazed samples (Group 1), was evaluated using a modified testing protocol based on ISO 6872:2024. Che...

  • Article
  • Open Access
1,166 Views
14 Pages

Eco-Friendly Synthesis of Chitosan–Fatty Acid Nano Micelles and Their Differential Antibacterial Activity Against Escherichia coli and Bacillus subtilis

  • Alfio Pulvirenti,
  • Valentina Verdoliva,
  • Viviana De Luca,
  • Serena Traboni,
  • Clemente Capasso and
  • Stefania De Luca

Chitosan-based nanoparticles were prepared using an eco-friendly chemical procedure that conjugates natural fatty acids to the backbone of chitosan. This consists of reacting two molecules in the absence of a solvent and using microwaves to promote t...

  • Article
  • Open Access
2 Citations
2,408 Views
22 Pages

Stress Assessment of Abutment-Free and Three Implant–Abutment Connections Utilizing Various Abutment Materials: A 3D Finite Element Study of Static and Cyclic Static Loading Conditions

  • Maryam H. Mugri,
  • Nandalur Kulashekar Reddy,
  • Mohammed E. Sayed,
  • Khurshid Mattoo,
  • Osama Mohammed Qomari,
  • Mousa Mahmoud Alnaji,
  • Waleed Abdu Mshari,
  • Firas K. Alqarawi,
  • Saad Saleh AlResayes and
  • Raghdah M. Alshaibani

Background: The implant–abutment interface has been thoroughly examined due to its impact on the success of implant healing and longevity. Removing the abutment is advantageous, but it changes the biomechanics of the implant fixture and restora...

  • Article
  • Open Access
2,188 Views
11 Pages

A Comparison Between Two Bearing Surfaces for Total Hip Arthroplasty—Ceramic-on-Ceramic and Metal–Polycarbonate–Urethane—A Pseudo-Randomized Study

  • Daniel Donaire Hoyas,
  • Eladio Jiménez Mejías,
  • Jesús Moreta,
  • Manuel Sumillera García,
  • Alberto Albert Ullibarri and
  • Jorge Albareda Albareda

Background: Polycarbonate–urethane (PCU) is a recently developed bearing surface used in prosthetic hip surgery. It offers several theoretical advantages, including an elasticity modulus similar to that of natural cartilage, good lubrication pr...

  • Systematic Review
  • Open Access
2 Citations
3,573 Views
14 Pages

The Current State of 3D-Printed Prostheses Clinical Outcomes: A Systematic Review

  • Huthaifa Atallah,
  • Titeana Qufabz,
  • Rabee Naeem,
  • Hadeel R. Bakhsh,
  • Giorgio Ferriero,
  • Dorottya Varga,
  • Evelin Derkács and
  • Bálint Molics

Introduction: 3D-printing is an emerging technology in the field of prosthetics, offering advantages such as cost-effectiveness, ease of customization, and improved accessibility. While previous reviews have focused on limited aspects, the aim of thi...

  • Systematic Review
  • Open Access
1 Citations
1,311 Views
16 Pages

Background/Objective: Clinical statistics show that bacterial infection is a major driver of implant failure. To enhance antibacterial performance, some metallic elements, such as silver (Ag), zinc (Zn), and copper (Cu), are commonly used to modify t...

  • Article
  • Open Access
1,302 Views
12 Pages

Tamibarotene (Am80) is a promising anti-tumor drug that induces the expression of Meflin (a glycosylphosphatidyl inositol-anchored protein) in cancer-associated fibroblasts, thereby improving the tumor microenvironment. However, Am80, which is approv...

  • Systematic Review
  • Open Access
1 Citations
2,332 Views
19 Pages

Autologous Tooth-Derived Biomaterials in Alveolar Bone Regeneration: A Systematic Review of Clinical Outcomes and Histological Evidence

  • Angelo Michele Inchingolo,
  • Grazia Marinelli,
  • Francesco Inchingolo,
  • Roberto Vito Giorgio,
  • Valeria Colonna,
  • Benito Francesco Pio Pennacchio,
  • Massimo Del Fabbro,
  • Gianluca Tartaglia,
  • Andrea Palermo and
  • Gianna Dipalma
  • + 1 author

Background: Autologous tooth-derived grafts have recently gained attention as an innovative alternative to conventional biomaterials for alveolar ridge preservation (ARP) and augmentation (ARA). Their structural similarity to bone and osteoinductive...

  • Article
  • Open Access
1,570 Views
15 Pages

Comparison of Wound Healing Efficiency Between Bacterial Cellulose Dry Membrane and Commercial Dressings

  • Wei-Wen Sung,
  • Yu-Jing Zeng,
  • Tsung-Ming Yeh,
  • Yao-Yuan Chen,
  • Min-Kung Hsu,
  • Sung-Pin Tseng and
  • Hsian-Yu Wang

The development of dressing materials mainly protects the wound, prevents infection, and assists in wound healing. Apart from the most common gauze on the market, different dressing materials can accelerate wound healing. Bacterial cellulose (BC) dre...

  • Article
  • Open Access
1,058 Views
14 Pages

The intervertebral disc (IVD) is a biphasic tissue in which the extracellular matrix (ECM) acts as a structural scaffold and regulates hydration and solute transport. The influence of hydration on the swelling and mechanical properties of the IVD, pa...

  • Review
  • Open Access
1,783 Views
21 Pages

Background: The aim of this bibliometric study was to evaluate publication trends in the most frequently cited clinical trials on the treatment of gingival recession, taking into account the augmentation materials used. Methods: A Web of Science sear...

  • Article
  • Open Access
2 Citations
1,742 Views
26 Pages

Magnesium Ion-Mediated Regulation of Osteogenesis and Osteoclastogenesis in 2D Culture and 3D Collagen/Nano-Hydroxyapatite Scaffolds for Enhanced Bone Repair

  • Sílvia Sá Paiva,
  • Avelino Ferreira,
  • Eavan Pakenham,
  • Kulwinder Kaur,
  • Brenton Cavanagh,
  • Fergal J. O’Brien and
  • Ciara M. Murphy

29 September 2025

Bone regeneration depends on a delicate balance between osteoblast-driven bone formation and osteoclast-mediated resorption, coordinated by complex biochemical cues. Magnesium (Mg2+) is known to modulate these processes. However, despite extensive re...

  • Article
  • Open Access
1 Citations
1,075 Views
25 Pages

Metal Ion Release from PEO-Coated Ti6Al4V DMLS Alloy for Orthopedic Implants

  • Shaghayegh Javadi,
  • Laura Castro,
  • Raúl Arrabal and
  • Endzhe Matykina

28 September 2025

This study investigates the influence of plasma electrolytic oxidation (PEO) on corrosion resistance of Ti6Al4V alloys produced by direct metal laser sintering (DMLS) for orthopedic implants. PEO (300 s) and flash-PEO (60 s) coatings containing Si, C...

  • Article
  • Open Access
2 Citations
1,423 Views
20 Pages

Enhanced Osteogenesis by Combining Exogenous BMPs with Hydroxyapatite/Aragonite Bone Grafts: In Vitro and In Vivo Studies

  • Hong Lu,
  • Ines Sousa dos Santos,
  • Emma Steijvers,
  • Miriam Lazim,
  • Victoria Higginbotham,
  • Baichuan Wang,
  • Zengwu Shao,
  • Venkateswarlu Kanamarlapudi and
  • Zhidao Xia

26 September 2025

Synthetic biomaterials are widely used as bone graft substitutes, but their osteogenic capacity is limited as they lack osteogenic growth factors. This study aimed to enhance the osteogenesis of a novel hydroxyapatite/aragonite (HAA) biomaterial by i...

  • Systematic Review
  • Open Access
1,613 Views
16 Pages

26 September 2025

Background: The growing interest in the field of platelet-rich fibrins has led to the development of novel generations of these concentrates, with one of the newest additions being advanced platelet-rich fibrin plus (A-PRF+). The updated centrifuge p...

  • Article
  • Open Access
1 Citations
1,542 Views
18 Pages

Effect of Cleaning Protocols on Surface Roughness of Current Polymeric Denture Materials

  • Lisa Brinkmann,
  • Florian Fuchs,
  • Martin Rosentritt,
  • Oliver Schierz,
  • Andreas Koenig and
  • Daniel R. Reissmann

24 September 2025

Surface roughness influences biofilm adhesion on denture base materials, impacting oral health. Despite advances in polymeric denture materials, the effects of common cleaning protocols on their surface texture remain inadequately characterized. This...

  • Article
  • Open Access
1,476 Views
21 Pages

Development of Bioceramic Bone-Inspired Scaffolds Through Single-Step Melt-Extrusion 3D Printing for Segmental Defect Treatment

  • Aikaterini Dedeloudi,
  • Pietro Maria Bertelli,
  • Laura Martinez-Marcos,
  • Thomas Quinten,
  • Imre Lengyel,
  • Sune K. Andersen and
  • Dimitrios A. Lamprou

23 September 2025

The increasing demand for novel tissue engineering (TE) applications in bone tissue regeneration underscores the importance of exploring advanced manufacturing techniques and biomaterials for personalised treatment approaches. Three-dimensional print...

  • Article
  • Open Access
2 Citations
2,088 Views
25 Pages

Eighty-Four-Month Clinical Outcomes of Autologous Dentin Graft Using Tooth Transformer® and Concentrated Growth Factors in Maxillary Atrophy: A Retrospective Study of 31 Patients

  • Gianna Dipalma,
  • Alessio Danilo Inchingolo,
  • Francesca Calò,
  • Rosalba Lagioia,
  • Paola Bassi,
  • Elisabetta de Ruvo,
  • Francesco Inchingolo,
  • Andrea Palermo,
  • Grazia Marinelli and
  • Angelo Michele Inchingolo

23 September 2025

Aim: This retrospective observational clinical cohort study evaluated 84-month clinical and radiographic outcomes of a regenerative protocol combining autologous dentin grafts processed with the Tooth Transformer® device and Concentrated Growth F...

  • Review
  • Open Access
1 Citations
3,191 Views
31 Pages

Organ-Specific Strategies in Bioprinting: Addressing Translational Challenges in the Heart, Liver, Kidney, and Pancreas

  • Mohamad Al Qassab,
  • Moustafa Merheb,
  • Safaa Sayadi,
  • Pia Salloum,
  • Zeina Dabbousi,
  • Anthony Bayeh,
  • Frederic Harb,
  • Sami Azar and
  • Hilda E. Ghadieh

23 September 2025

Organ bioprinting is a rapidly evolving field designed to address the persistent shortage of donor organs by engineering patient-specific tissues that replicate the function and structure of natural organs. Despite significant technological advanceme...

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J. Funct. Biomater. - ISSN 2079-4983