QTL Mapping for Wheat Seed Dormancy in a Yangmai16/Zhongmai895 Double Haploid Population
Abstract
:1. Introduction
2. Results
2.1. Phenotype Characterization and Analysis
2.2. QTL Mapping for Seed Dormancy and Grain Color
2.3. Effects of Qdor.hzau-3D.2 on Seed Dormancy in DH Population
2.4. QTL Validation of Qdor.hzau-3D.2
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Trial Environments
4.2. Phenotype Assessment
4.3. Genotyping and Linkage Mapping
4.4. Statistical Analysis
4.5. QTL Analysis
4.6. KASP Marker Development and Validation
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Environment | Parent | DH Population | ||||
---|---|---|---|---|---|---|
YM16 (%) | ZM895 (%) | Min (%) | Max (%) | Mean (%) | Heritability | |
LY21 | 7.52 **** | 76.38 | 0.00 | 96.38 | 28.95 | 0.94 |
LY22 | 4.95 **** | 78.67 | 0.10 | 74.00 | 16.83 | 0.78 |
WH22 | 3.11 **** | 74.79 | 0.10 | 93.52 | 17.43 | 0.87 |
XY22 | 4.48 **** | 78.48 | 0.29 | 92.57 | 25.02 | 0.84 |
BLUE | 9.00 **** | 80.00 | 5.54 | 79.12 | 25.90 | 0.88 |
Environment | Parent | DH Population | ||||
---|---|---|---|---|---|---|
YM16 (%) | ZM895 (%) | Min (%) | Max (%) | Mean (%) | Heritability | |
LY21 | 19.33 **** | 88.67 | 0.00 | 98.67 | 43.12 | 0.84 |
LY22 | 9.33 **** | 92.67 | 0.67 | 88.67 | 28.58 | 0.75 |
WH22 | 6.00 **** | 80.44 | 0.67 | 95.33 | 25.07 | 0.84 |
XY22 | 9.33 **** | 87.33 | 1.33 | 97.33 | 34.07 | 0.79 |
BLUE | 14.95 **** | 90.23 | 7.49 | 88.02 | 36.59 | 0.88 |
QTL | Trait | Env | Position | Left Marker | Right Marker | LOD | PVE (%) | Add |
---|---|---|---|---|---|---|---|---|
Qdor.hzau-2A | GI | LY21 | 57 | AX-109313988 | AX-109575090 | 3.5 | 6.12 | −0.06 |
Qdor.hzau-3A | GI | LY21 | 176 | AX-94633769 | AX-109554358 | 3.61 | 6.72 | −0.06 |
LY22 | 182 | AX-94455457 | AX-111235128 | 7.08 | 11.67 | −0.06 | ||
XY22 | 185 | AX-95119177 | AX-111670577 | 6.2 | 8.89 | −0.07 | ||
BLUE | 185 | AX-95119177 | AX-111670577 | 8.54 | 11.78 | −0.06 | ||
GP | LY21 | 176 | AX-94633769 | AX-109554358 | 3.56 | 6.46 | −0.07 | |
LY22 | 182 | AX-94455457 | AX-111235128 | 6.2 | 9.63 | −0.08 | ||
WH22 | 185 | AX-95119177 | AX-111670577 | 3.57 | 4.67 | −0.05 | ||
XY22 | 185 | AX-95119177 | AX-111670577 | 5.98 | 8.47 | −0.08 | ||
BLUE | 185 | AX-95119177 | AX-111670577 | 7.44 | 10.01 | −0.07 | ||
Qdor.hzau-3D.1 | GI | LY21 | 176 | AX-110967455 | AX-109649069 | 8.15 | 15.31 | −0.09 |
LY22 | 180 | AX-110194478 | AX-110515593 | 13.67 | 23.27 | −0.08 | ||
WH22 | 182 | AX-94543851 | AX-94850967 | 12.71 | 28.3 | −0.1 | ||
XY22 | 176 | AX-110967455 | AX-109649069 | 14.03 | 21.99 | −0.11 | ||
BLUE | 176 | AX-110967455 | AX-109649069 | 17.93 | 28.14 | −0.09 | ||
GP | LY21 | 180 | AX-110194478 | AX-110515593 | 11.05 | 21.08 | −0.13 | |
LY22 | 180 | AX-110194478 | AX-110515593 | 13.93 | 22.95 | −0.12 | ||
WH22 | 182 | AX-94543851 | AX-94850967 | 19.37 | 31.94 | −0.13 | ||
XY22 | 176 | AX-110967455 | AX-109649069 | 13.22 | 20.01 | −0.12 | ||
BLUE | 180 | AX-110194478 | AX-110515593 | 18.49 | 28.97 | −0.12 | ||
Qdor.hzau-3D.2 | GI | LY21 | 229 | AX-110937331 | AX-108883716 | 3.9 | 7.24 | 0.06 |
LY22 | 227 | AX-110937331 | AX-108883716 | 3.83 | 6.59 | 0.04 | ||
XY22 | 206 | AX-94926374 | AX-110536745 | 6.38 | 9.49 | 0.07 | ||
BLUE | 230 | AX-110937331 | AX-108883716 | 5.7 | 7.49 | 0.05 | ||
GP | LY21 | 228 | AX-110937331 | AX-108883716 | 3.84 | 7.32 | 0.08 | |
LY22 | 226 | AX-110937331 | AX-108883716 | 3.35 | 5.6 | 0.06 | ||
WH22 | 228 | AX-110937331 | AX-108883716 | 3.4 | 4.98 | 0.05 | ||
XY22 | 206 | AX-94926374 | AX-110536745 | 6.97 | 10.34 | 0.09 | ||
BLUE | 230 | AX-110937331 | AX-108883716 | 7.36 | 9.94 | 0.07 | ||
Qdor.hzau-4D | GP | LY22 | 43 | AX-108763556 | AX-110984743 | 3.26 | 4.75 | 0.05 |
Qdor.hzau-5B | GP | LY21 | 124 | AX-94615114 | AX-94962457 | 2.58 | 4.04 | 0.06 |
Qdor.hzau-5D | GI | WH22 | 170 | AX-110586161 | AX-108740226 | 4.68 | 9.26 | 0.06 |
GP | WH22 | 170 | AX-110586161 | AX-108740226 | 5.98 | 8.13 | 0.07 |
QTL | Env | Position | Left Marker | Right Marker | LOD | PVE (%) | Add |
---|---|---|---|---|---|---|---|
Qgc.hzau-3A | LY21 | 185 | AX-95119177 | AX-111670577 | 9.76 | 19.25 | 0.17 |
XY22 | 185 | AX-95119177 | AX-111670577 | 10.35 | 21.11 | 0.19 | |
Qgc.hzau-3D | LY21 | 182 | AX-94543851 | AX-94850967 | 14.99 | 30.44 | 0.23 |
XY22 | 180 | AX-110194478 | AX-110515593 | 14.72 | 30.25 | 0.23 |
Type | Num | GI (%) | GP (%) | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
LY21 | LY22 | WH22 | XY22 | BLUE | LY21 | LY22 | WH22 | XY22 | BLUE | ||
Class1 | 16 | 63.00 | 46.12 | 44.77 | 62.95 | 57.96 | 82.00 | 67.00 | 55.25 | 74.00 | 73.49 |
Class2 | 20 | 47.51 | 33.42 | 35.08 | 45.19 | 45.12 | 70.00 | 49.58 | 45.96 | 58.00 | 59.99 |
Class3 | 35 | 23.01 | 10.76 | 9.33 | 18.57 | 19.24 | 34.00 | 20.32 | 15.87 | 24.00 | 28.00 |
Class4 | 23 | 21.46 | 9.28 | 5.02 | 13.71 | 15.82 | 33.00 | 18.58 | 9.09 | 21.00 | 24.12 |
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Guo, G.; Xu, S.; Chen, H.; Hao, Y.; Mao, H. QTL Mapping for Wheat Seed Dormancy in a Yangmai16/Zhongmai895 Double Haploid Population. Plants 2023, 12, 759. https://doi.org/10.3390/plants12040759
Guo G, Xu S, Chen H, Hao Y, Mao H. QTL Mapping for Wheat Seed Dormancy in a Yangmai16/Zhongmai895 Double Haploid Population. Plants. 2023; 12(4):759. https://doi.org/10.3390/plants12040759
Chicago/Turabian StyleGuo, Gang, Shuhao Xu, Hao Chen, Yuanfeng Hao, and Hailiang Mao. 2023. "QTL Mapping for Wheat Seed Dormancy in a Yangmai16/Zhongmai895 Double Haploid Population" Plants 12, no. 4: 759. https://doi.org/10.3390/plants12040759