Morphological and Biophysical Study of S100A9 Protein Fibrils by Atomic Force Microscopy Imaging and Nanomechanical Analysis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Preparation
2.2. Mica Preparation and AFM Measurements
2.3. Data Analysis
2.3.1. Height Measurements
2.3.2. Mechanical Properties
3. Results and Discussion
3.1. The S100A9 Protein
3.2. Formation of Fibrillar Oligomers
3.3. Fibril Height
3.4. Period and Fibril Length
3.5. Mechanical Properties and Biophysical Characterization of Fibrillar Oligomers
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
AD | Alzheimer’s disease |
AFM | Atomic force microscopy |
TEM | Transmission electron microscopy |
SPM | Scanning probe microscopy |
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Carapeto, A.P.; Marcuello, C.; Faísca, P.F.N.; Rodrigues, M.S. Morphological and Biophysical Study of S100A9 Protein Fibrils by Atomic Force Microscopy Imaging and Nanomechanical Analysis. Biomolecules 2024, 14, 1091. https://doi.org/10.3390/biom14091091
Carapeto AP, Marcuello C, Faísca PFN, Rodrigues MS. Morphological and Biophysical Study of S100A9 Protein Fibrils by Atomic Force Microscopy Imaging and Nanomechanical Analysis. Biomolecules. 2024; 14(9):1091. https://doi.org/10.3390/biom14091091
Chicago/Turabian StyleCarapeto, Ana P., Carlos Marcuello, Patrícia F. N. Faísca, and Mário S. Rodrigues. 2024. "Morphological and Biophysical Study of S100A9 Protein Fibrils by Atomic Force Microscopy Imaging and Nanomechanical Analysis" Biomolecules 14, no. 9: 1091. https://doi.org/10.3390/biom14091091