Exogenous Application of Methyl Jasmonate and Salicylic Acid Mitigates Drought-Induced Oxidative Damages in French Bean (Phaseolus vulgaris L.)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material, Experimental Conditions, and Treatments
2.2. Pot Capacity and Water Content of the Experimental Soil
2.3. Growth Parameters
2.4. Physiological Parameters
2.4.1. Leaf Chlorophyll and Pigment Content
2.4.2. Canopy Temperature Depression
2.4.3. Leaf Relative Water Content
2.4.4. Cell Membrane Stability
2.5. Biochemical Observations
2.5.1. Proline Content
2.5.2. Oxidative Stress Indicators
2.5.3. Extraction and Quantitation of Soluble Protein
2.5.4. Assays of Enzymatic Activities
2.6. Statistical Analysis
3. Results
3.1. Soil Water Content
3.2. MeJA and SA Enhanced Plant Growth
3.3. Impact of MeJA and SA on Photosynthetic Pigments
3.4. MeJA and SA Improved Physiological Traits
3.5. MeJA and SA Suppressed the Generation of Oxidative Stress Indicators
3.6. Antioxidant Enzyme Activities and MeJA and SA Pre-Treatment
3.7. Comparative Assessment of Responses across Treatments
3.8. Principal Component Analysis (PCA)
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Treatment | Root Length (cm) | Shoot Length (cm) | Root Dry Weight (g) | Shoot Dry Weight (g) | Leaf Dry Weight (g) | Total Dry Weight (g) |
---|---|---|---|---|---|---|
Control | 58.63 ± 3.45 a | 36.49 ± 0.87 a | 2.88 ± 0.26 a | 9.04 ± 0.74 a | 9.93 ± 0.77 a | 21.86 ± 1.62 a |
Drought (D) | 55.69 ± 1.23 a | 21.27 ± 0.65 c | 1.38 ± 0.12 b | 2.77 ± 0.21 c | 2.61 ± 0.28 d | 6.76 ± 0.35 c |
D + MeJA | 57.59 ± 2.13 a | 22.10 ± 0.49 c | 1.53 ± 0.17 b | 3.55 ± 0.19 c | 3.91 ± 0.37 b c | 8.99 ± 0.64 c |
D + SA | 58.91 ± 2.55 a | 22.46 ± 0.63 c | 1.54 ± 0.18 b | 3.69 ± 0.49 c | 3.62 ± 0.13 c d | 8.85 ± 0.65 c |
D + MeJA + SA | 59.81 ± 1.07 a | 25.63 ± 0.55 b | 1.86 ± 0.19 b | 5.09 ± 0.28 b | 4.89 ± 0.12 b | 11.84 ± 0.23 b |
Treatments | SPAD | Leaf Pigments (mg g−1 Fresh Weight) | |||
---|---|---|---|---|---|
Chl a | Chl b | Total Chl | Carotenoids | ||
Control | 49.85 ± 0.49 a | 1.39 ± 0.03 a | 0.49 ± 0.02 a | 1.97 ± 0.06 a | 0.50 ± 0.03 a |
Drought (D) | 39.65 ± 1.18 c | 0.65 ± 0.10 c | 0.24 ± 0.05 c | 0.98 ± 0.07 c | 0.26 ± 0.01 c |
D + MeJA | 43.65 ± 0.93 b | 0.87 ± 0.11 bc | 0.29 ± 0.04 bc | 1.25 ± 0.17 bc | 0.34 ± 0.05 bc |
D + SA | 44.35 ± 1.52 b | 0.91 ± 0.16 bc | 0.30 ± 0.07 bc | 1.30 ± 0.25 bc | 0.33 ± 0.06 bc |
D + MeJA + SA | 48.18 ± 0.94 a | 1.12 ± 0.06 b | 0.39 ± 0.02 ab | 1.61 ± 0.08 ab | 0.42 ± 0.03 ab |
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Mohi-Ud-Din, M.; Talukder, D.; Rohman, M.; Ahmed, J.U.; Jagadish, S.V.K.; Islam, T.; Hasanuzzaman, M. Exogenous Application of Methyl Jasmonate and Salicylic Acid Mitigates Drought-Induced Oxidative Damages in French Bean (Phaseolus vulgaris L.). Plants 2021, 10, 2066. https://doi.org/10.3390/plants10102066
Mohi-Ud-Din M, Talukder D, Rohman M, Ahmed JU, Jagadish SVK, Islam T, Hasanuzzaman M. Exogenous Application of Methyl Jasmonate and Salicylic Acid Mitigates Drought-Induced Oxidative Damages in French Bean (Phaseolus vulgaris L.). Plants. 2021; 10(10):2066. https://doi.org/10.3390/plants10102066
Chicago/Turabian StyleMohi-Ud-Din, Mohammed, Dipa Talukder, Motiar Rohman, Jalal Uddin Ahmed, S. V. Krishna Jagadish, Tofazzal Islam, and Mirza Hasanuzzaman. 2021. "Exogenous Application of Methyl Jasmonate and Salicylic Acid Mitigates Drought-Induced Oxidative Damages in French Bean (Phaseolus vulgaris L.)" Plants 10, no. 10: 2066. https://doi.org/10.3390/plants10102066