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Volume 10
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10.3390/plants10112459
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Article

Establishment of a Novel and Efficient Agrobacterium-Mediated in Planta Transformation System for Passion Fruit (Passiflora edulis)

by
Hafiz Muhammad Rizwan
1,
Qiang Yang
1,
Ahmed Fathy Yousef
1,2,
Xiaoxue Zhang
1,
Yasir Sharif
3,
Jia Kaijie
1,
Meng Shi
1,
Han Li
1,
Nigarish Munir
1,
Xuelian Yang
1,
Xiaoxia Wei
4,
Ralf Oelmüller
1,5,
Chunzhen Cheng
1,* and
Faxing Chen
1,*
1
College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2
Department of Horticulture, College of Agriculture, University of Al-Azhar (Branch Assiut), Assiut 71524, Egypt
3
Institute of Oil Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China
4
Fruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350002, China
5
Matthias Schleiden Institute, Plant Physiology, Friedrich-Schiller-University Jena, Dornburger Str. 159, 07743 Jena, Germany
*
Authors to whom correspondence should be addressed.
Plants 2021, 10(11), 2459; https://doi.org/10.3390/plants10112459
Submission received: 24 September 2021 / Revised: 31 October 2021 / Accepted: 3 November 2021 / Published: 15 November 2021
(This article belongs to the Special Issue Plant Regeneration and Organ Formation)
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Abstract

Passion fruit (Passiflora edulis) is an important fruit crop with high economic value. Genetic engineering plays an important role in crop improvement with desired traits and gene functional studies. The lack of a simple, efficient, and stable transformation system for passion fruit has greatly limited gene functional studies. In this study, a simple and efficient Agrobacterium-mediated in planta transformation system for passion fruit was established, using Agrobacterium virulent strain EHA105 harboring the binary vectors pCAMBIA1301 and pCAMBIA1302 with GUS and GFP reporter genes. The system requires less time and labor costs than conventional transformation systems, and no additional phytohormones and sterile conditions are required. Regeneration efficiency of 86% and transformation efficiency of 29% were achieved, when the wounds were wrapped with Parafilm and the plants were kept in darkness for 15 days. Approximately 75% of the regenerated plants had a single shoot and 26% multiple shoots. The transformation was confirmed at the DNA and RNA levels as well as by GUS staining and GFP fluorescent measurements. The developed protocol will contribute to the genetic improvement of passion fruit breeding.
Keywords: Passiflora edulis; dark incubation; parafilm wrapping; in planta; Agrobacterium-mediated transformation Passiflora edulis; dark incubation; parafilm wrapping; in planta; Agrobacterium-mediated transformation

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MDPI and ACS Style

Rizwan, H.M.; Yang, Q.; Yousef, A.F.; Zhang, X.; Sharif, Y.; Kaijie, J.; Shi, M.; Li, H.; Munir, N.; Yang, X.; et al. Establishment of a Novel and Efficient Agrobacterium-Mediated in Planta Transformation System for Passion Fruit (Passiflora edulis). Plants 2021, 10, 2459. https://doi.org/10.3390/plants10112459

AMA Style

Rizwan HM, Yang Q, Yousef AF, Zhang X, Sharif Y, Kaijie J, Shi M, Li H, Munir N, Yang X, et al. Establishment of a Novel and Efficient Agrobacterium-Mediated in Planta Transformation System for Passion Fruit (Passiflora edulis). Plants. 2021; 10(11):2459. https://doi.org/10.3390/plants10112459

Chicago/Turabian Style

Rizwan, Hafiz Muhammad, Qiang Yang, Ahmed Fathy Yousef, Xiaoxue Zhang, Yasir Sharif, Jia Kaijie, Meng Shi, Han Li, Nigarish Munir, Xuelian Yang, and et al. 2021. "Establishment of a Novel and Efficient Agrobacterium-Mediated in Planta Transformation System for Passion Fruit (Passiflora edulis)" Plants 10, no. 11: 2459. https://doi.org/10.3390/plants10112459

APA Style

Rizwan, H. M., Yang, Q., Yousef, A. F., Zhang, X., Sharif, Y., Kaijie, J., Shi, M., Li, H., Munir, N., Yang, X., Wei, X., Oelmüller, R., Cheng, C., & Chen, F. (2021). Establishment of a Novel and Efficient Agrobacterium-Mediated in Planta Transformation System for Passion Fruit (Passiflora edulis). Plants, 10(11), 2459. https://doi.org/10.3390/plants10112459

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