Translational Chickpea Genomics Consortium to Accelerate Genetic Gains in Chickpea (Cicer arietinum L.)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Marker-Assisted Backcrossing for Drought Tolerance and Fusarium wilt Resistance
2.2. Development of Trait-Associated Markers
2.3. Multi-Location Trials for Promising Chickpea Lines
2.4. Varietal Adoption through Farmer Participatory Varietal Selection (FPVS) Trials
3. Results
3.1. Genomics-Assisted Breeding
3.2. Mean Performance and Stability of Elite Breeding Lines for Grain Yield in Multi-Location Trials
3.3. Promising Desi Lines for Grain Yield Identified through Multi-Location Trials
3.4. Promising Kabuli Lines for Grain Yield Identified through Multi-Location Trials
3.5. Enhancing Varietal Adoption through FPVS Trials
3.6. Selection of High-Performing Varieties in Different States
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Intertek ID | SNP ID | Trait | Sequence * |
---|---|---|---|
snpCA0001 | CKAM2210 | Drought | TTAAACTCACTTACCCTCTTTCCTTTCCATTTCCTTTCTTTCAAAATTCTCCTATATCCT[G/T]CTAATACAGATACTTTGCAACCCATTTTTTTTGTCAACAAAGTGTTATTGGGTGAGTTCA |
snpCA0022 | CKAM2227 | Drought | CGAGGCCCAAATCCAAAACCGGATTCAAATTCATTTTAAATATCCGGTTAAAATCATATG[A/G]TTATAATTTGGTTTATTTATAAACCGGTTGGATAACCACTTATGTTTTATATTTGGATTT |
snpCA0023 | CKAM2228 | Drought | CATCTGAAGATTATGTGCAGCTTAAGGTGTTGGCGGCAATTCAAGGGGACGCTAGTGTTT[C/G]TAAGGATGACAAAATTGAGCATTTGTTCTTTTCCTTAATGTTTTTTCAAAAACTCTCAAT |
snpCA0004 | CKAM2179 | Drought | ATGTCTTCGGCTTCCAGATTTGTGTTTGGTGACATGACCGAAGAAAGCTTGAAATGAGCT[G/T]ATAGTGAAGAGCTCACTGCCTTTGATTCACACATATTGAATCTATTTAGAACCTTTCCAA |
snpCA0006 | CKAM2182 | Drought | AACCACATGAAGAAAATAAATTATGTAAAATGTGTTGTTTCTTCGAATCAACTATGGTAT[C/T]GAGGCTATTCTGGATATCGAAGGGACATAATGAAAGAGAGAGTAGTGGCTTCGAAATGCG |
snpCA0021 | CKAM2226 | Drought | CGCTATTAAGTACAAAAAATTGTCAAATAGCGGTTATAGCAATCTATAGCGTTGTTGCTT[A/T]GAGGAATATAAATAAACCACTATTTTTCACAATCTGCGATTCACAAAATTGGTATGTATG |
snpCA0018 | CKAM2223 | Drought | TGAACAAAAACTTCTACGTGATCAGTTTGTCATATTTCACAAAAAAAAAAAAAAGGAATA[A/T]ATGCAATATATGCGGCTCAATTGGATGTTGTAACCATGGATTCTATTGATTAGTGGTCAA |
snpCA0166 | FW2_30366103 | Fusarium wilt | TTCTATTATATTTTGATACTGTGGAGAATCATAGTCAAATACAATTGATA[C/A]ATACAACTTCAATTGGCCATAGAGGTCAGAGACTTCAAAAACTTTGATGT |
snpCA0168 | FW2_30366146 | Fusarium wilt | AAATACAATTGATACATACAACTTCAATTGGCCATAGAGGTCAGAGACTT[C/A]AAAAACTTTGATGTCGCAGCTCACATCACTATCACAATCACAATCACAAT |
State | Center | District | Variety | Number of FPVS Trials | Total FPVS Trials | ||
---|---|---|---|---|---|---|---|
2017–2018 | 2018–2019 | 2019–2020 | |||||
Andhra Pradesh | RARS-Nandyal | Anantapur, Kurnool and Prakasam | NBeG 47, NBeG 49 and NBeG3 | 30 | 90 | 90 | 210 |
Karnataka | ARS-Kalaburagi | Bijapur, Dharwad, Gadag and Kalaburagi | GBM 2, BGD103, JAKI 9218 and JG 11 | 20 | 70 | 51 | 141 |
Madhya Pradesh | RAKCA-Sehore | Indore, Sehore and Ujjain | RVG 202, RVG 203, RVKG 101 RVKG 102, RVG 204, RVG 205 and RVKG111 | 30 | 90 | 90 | 210 |
Maharashtra | MPKV-Rahuri | Ahmednagar, Pune and Solapur | Phule Vikram, RVG 203, RVG 202 and Kripa | 30 | 98 | 76 | 204 |
Uttar Pradesh | ICAR-IIPR, Kanpur | Jalaun, Mahoba and Fatehpur | JG 14, Ujjawal, Shubhra and RVG 202 | 25 | 93 | 116 | 234 |
Effect | Desi Lines | Kabuli Lines |
---|---|---|
Variance Components | Variance Components | |
Environment | 37.94 ** | 52.98 ** |
Replication (Environment) | 0.37 ** | 0 |
Block (Environment × Replication) | 0.004 * | 0 |
Genotype | 0.96 * | 0.73 |
Environment × Genotype | 8.76 ** | 15.93 ** |
Residual | 13.96 | 25.28 |
Desi Lines during 2016–2017 | Andhra Pradesh | Karnataka | Madhya Pradesh | Maharashtra |
---|---|---|---|---|
JG 2016-1614 | √ | √ | ||
IPC 2012-98 | √ | √ | ||
Kabuli during 2016–2017 | ||||
ICCX-060010-F3-BP-P17-BP-BP-BP-BP | √ | √ | ||
IPCK 2013-174 | √ | √ | ||
SAGL 152225 | √ | |||
SAGL 152289 | √ | |||
Desi lines during 2018–2019 | ||||
IPC 2015-105 | √ | √ | ||
IPC 2015-120 | √ | √ | ||
SAGL 152317 | √ | √ | ||
JG 2016-1614 | √ | √ | ||
JG 2016-634958 | √ | √ | ||
JG 2016-921814 | √ | √ | ||
Kabuli lines during 2018–2019 | ||||
IPCK 2014-98 | √ | √ | ||
SAGL 152289 | √ | √ | ||
ICCX-060010-F3-BP-P6-BP-BP-BP-BP | √ | √ |
State | Year | Variety | Kurnool | Prakasam | Anantapur | Mean |
---|---|---|---|---|---|---|
Andhra Pradesh | 2017–2018 | NBeG 3 | 686 | 413 | 492 | 530 |
NBeG 47 | 679 | 445 | 491 | 538 | ||
NBeG 49 | 857 | 488 | 561 | 635 | ||
2018–2019 | NBeG 49 | 1518 | 1040 | 1326 | 1295 | |
2019–2020 | NBeG 49 | 1810 | 1679 | 1606 | 1699 | |
NBeG 119 | 1716 | 1833 | 1161 | 1503 | ||
State | Year | Variety | Kalaburagi | Bijapur | Dharwad | Mean |
Karnataka | 2017–2018 | GBM 2 | 1565 | 1270 | 1016 | 1284 |
BGD 103 | 1490 | 1395 | 1232 | 1372 | ||
MNK 1 | 1105 | 1195 | 650 | 983 | ||
2018–2019 | GBM 2 | 1763 | 1628 | - | 1696 | |
2019–2020 | GBM 2 | 1795 | 1521 | - | 1658 | |
State | Year | Variety | Ujjain | Indore | Sehore | Mean |
Madhya Pradesh | 2017–2018 | RVG 202 | 1854 | 1852 | 2014 | 1907 |
RVG 203 | 1886 | 1830 | 1864 | 1860 | ||
RVGK 101 | 1429 | 1359 | 1737 | 1508 | ||
RVGK 102 | - | 1165 | 1537 | 1351 | ||
2019–2020 | RVG 205 | 1476 | 1212 | 1528 | 1405 | |
RVG 203 | 1965 | 1741 | 1610 | 1772 | ||
RVG 202 | 2080 | 2150 | 2085 | 2105 | ||
RVG 111 | 1548 | 1450 | 1654 | 1551 | ||
RVKG 101 | 1450 | 1633 | 1700 | 1594 | ||
State | Year | Variety | Ahmednagar | Pune | Solapur | Mean |
Maharashtra | 2017–2018 | Kripa | 315 | 320 | 509 | 381 |
Phule Vikram | 1380 | 1720 | 1658 | 1586 | ||
RVG 202 | 1211 | 1785 | 1809 | 1602 | ||
2018–2019 | Kripa | 1169 | 875 | - | 1022 | |
Phule Vikram | 1688 | 2000 | 1222 | 1637 | ||
RVG 202 | 1495 | 2062 | 1521 | 1693 | ||
RVG 203 | 1637 | 2015 | 1384 | 1679 | ||
2019–2020 | Phule Vikram | 1746 | 1298 | 987 | 1344 | |
State | Year | Variety | Fatehpur | Jalaun | Mahoba | Mean |
Uttar Pradesh | 2017–2018 | Shubhra | 850 | 1700 | - | 1275 |
RVG 202 | 1715 | 350 | - | 1033 | ||
2018–2019 | RVG 202 | 1846 | 938 | 1401 | 1395 | |
Shubhra | 3484 | 1853 | 1608 | 2315 | ||
JG 14 | 2388 | 1556 | 1392 | 1779 | ||
Ujjawal | 3718 | 2085 | 1529 | 2444 | ||
2019–2020 | RVG 202 | 650 | 869 | 1209 | 909 | |
RVG 203 | 629 | 610 | 1325 | 855 | ||
JG 14 | 608 | 786 | 1548 | 980 |
Effect | Andhra Pradesh (Nandyal) 2017–2018 | Karnataka (Kalaburagi) 2017–2018 | Madhya Pradesh (Sehore) 2017–2018 | Maharashtra (Rahuri) 2017–2018 | Maharashtra (Rahuri) 2018–2019 | Uttar Pradesh (Kanpur) 2017–2018 | Uttar Pradesh (Kanpur) 2018–2019 |
---|---|---|---|---|---|---|---|
District | 2.1 | 9.50 ** | 2.02 | 0.81 | 0.79 | 3.3 | 6.77 ** |
Variety | 7.26 ** | 89.79 ** | 27.47 ** | 0.08 | 0.02 | 3.67 | 0.26 |
District × Variety | 1.75 | 36.26 ** | 2 | 0.59 | 0.21 | 1.29 | 0.29 |
Residual | 0.25 | 0.52 | 1.57 | 10.78 | 7.47 | 14.87 | 14.98 |
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Palakurthi, R.; Jayalakshmi, V.; Kumar, Y.; Kulwal, P.; Yasin, M.; Kute, N.S.; Laxuman, C.; Yeri, S.; Vemula, A.; Rathore, A.; et al. Translational Chickpea Genomics Consortium to Accelerate Genetic Gains in Chickpea (Cicer arietinum L.). Plants 2021, 10, 2583. https://doi.org/10.3390/plants10122583
Palakurthi R, Jayalakshmi V, Kumar Y, Kulwal P, Yasin M, Kute NS, Laxuman C, Yeri S, Vemula A, Rathore A, et al. Translational Chickpea Genomics Consortium to Accelerate Genetic Gains in Chickpea (Cicer arietinum L.). Plants. 2021; 10(12):2583. https://doi.org/10.3390/plants10122583
Chicago/Turabian StylePalakurthi, Ramesh, Veera Jayalakshmi, Yogesh Kumar, Pawan Kulwal, Mohammad Yasin, Nandkumar Surendra Kute, Chinchole Laxuman, Sharanabasappa Yeri, Anilkumar Vemula, Abhishek Rathore, and et al. 2021. "Translational Chickpea Genomics Consortium to Accelerate Genetic Gains in Chickpea (Cicer arietinum L.)" Plants 10, no. 12: 2583. https://doi.org/10.3390/plants10122583
APA StylePalakurthi, R., Jayalakshmi, V., Kumar, Y., Kulwal, P., Yasin, M., Kute, N. S., Laxuman, C., Yeri, S., Vemula, A., Rathore, A., Samineni, S., Soren, K. R., Mondal, B., Dixit, G. P., Bharadwaj, C., Chaturvedi, S. K., Gaur, P. M., Roorkiwal, M., Thudi, M., ... Varshney, R. K. (2021). Translational Chickpea Genomics Consortium to Accelerate Genetic Gains in Chickpea (Cicer arietinum L.). Plants, 10(12), 2583. https://doi.org/10.3390/plants10122583