Interplay between Proline Metabolism and ROS in the Fine Tuning of Root-Meristem Size in Arabidopsis
Abstract
:1. Introduction
2. Results
2.1. Proline Affects the Local Distribution of Superoxide and Hydrogen Peroxide in the Arabidopsis Root
2.2. Proline and Hydrogen Peroxide Have Similar Effects on Root Meristem Size
2.3. The Effect of Proline on Root Meristem Size Is Mediated by Hydrogen Peroxide
2.4. Role of Proline Catabolism in Proline-Mediated Root Elongation
2.5. Proline Enhances the Activity of H2O2-Scavenging Enzymes
3. Discussion
4. Materials and Methods
4.1. Plant Growth Conditions and Genetic Crosses
4.2. Analysis of the Root Meristem
4.3. Proline Analysis
4.4. Molecular Techniques
4.5. Enzymatic Assays
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Primer Name | Sequence | Gene |
---|---|---|
RCH1_for | GGCGTGTTGGCGGTTATACG | At5g48940 |
RCH1_rev | ATCCCGGAGCAACCTTTCCC | |
CYCB1;1_for | TGGTAGCTGCTTCTGCAATC | At4g37490 |
CYCB1;1_rev | AGCTTTGCACAGTCCATGAG | |
CAT1_for | TCTCCCACCACCCAGAGAGT | At1g20630 |
CAT1_rev | AGCTTCCTCATCCGACAGGC | |
APX1_for | CGTCCATTTTAAAGCCGTGCG | At1g07890 |
APX1_rev | CGAGTGGCTGGCACGAGTAA | |
DHAR1_for | CCCACTGGTGGGTGGAGAAT | At3g24170 |
DHAR1_rev | CGGACAGTCGCCGAGATGAT | |
CSD1_for | AGCAGTGAGGGTGTTACGGG | At1g08830 |
CSD1_rev | GGGGCACCGTGTGTTTTACC | |
MSD1_for | TTCAACGGCGGAGGTCATGT | At3g10920 |
MSD1_rev | AGCCACCATCCTGAGCCTTG | |
Per_39_for | AAGCTTGCTCCTCCGAATCT | At4g11290 |
Per_39_rev | GTCGGTCCACCAATAGCAAC | |
Per_40_for | CTTGGCCTTTCACAAACCGA | At4g16270; |
Per_40_rev | TGGTTGTCCAGTTTGCAGTG | |
Per_57_for | AAGCTTGCTCCTCCGAATCT | At5g17820 |
Per_57_rev | GTCGGTCCACCAATAGCAAC |
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Bauduin, S.; Latini, M.; Belleggia, I.; Migliore, M.; Biancucci, M.; Mattioli, R.; Francioso, A.; Mosca, L.; Funck, D.; Trovato, M. Interplay between Proline Metabolism and ROS in the Fine Tuning of Root-Meristem Size in Arabidopsis. Plants 2022, 11, 1512. https://doi.org/10.3390/plants11111512
Bauduin S, Latini M, Belleggia I, Migliore M, Biancucci M, Mattioli R, Francioso A, Mosca L, Funck D, Trovato M. Interplay between Proline Metabolism and ROS in the Fine Tuning of Root-Meristem Size in Arabidopsis. Plants. 2022; 11(11):1512. https://doi.org/10.3390/plants11111512
Chicago/Turabian StyleBauduin, Sara, Martina Latini, Irene Belleggia, Marta Migliore, Marco Biancucci, Roberto Mattioli, Antonio Francioso, Luciana Mosca, Dietmar Funck, and Maurizio Trovato. 2022. "Interplay between Proline Metabolism and ROS in the Fine Tuning of Root-Meristem Size in Arabidopsis" Plants 11, no. 11: 1512. https://doi.org/10.3390/plants11111512