Response Mechanisms of “Hass” Avocado to Sequential 1–methylcyclopropene Applications at Different Maturity Stages during Cold Storage
Abstract
:1. Introduction
2. Results
2.1. Ripening Related Physiological Parameters
2.1.1. Ripening Parameters
2.1.2. Physiological Parameters
2.2. Fruit Characteristics at the 1–MCP Application Time
2.2.1. Ripening Parameters
2.2.2. Physiological Parameters
2.2.3. Transcriptomic Analysis
2.2.4. Expression Patterns of Genes Involved in Ethylene Biosynthesis
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Dry Matter (DM)
4.3. 1–MCP Applications
4.4. Mesocarp Firmness
4.5. Skin Color
4.6. Respiration and Ethylene Production Rates
4.7. Bioinformatic Analysis
4.7.1. RNA-Seq Experiment
4.7.2. Sequencing Data Analysis
4.7.3. Differential Gene Expression Analysis
4.8. cDNA Synthesis and Real-Time Quantitative PCR Assays
4.9. Experimental Design and Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Harvest | Trial | Up | Down |
---|---|---|---|
LDM | 14 d at 5 °C vs. Harvest | 701 | 657 |
>21 d at 5 °C vs. Harvest | 1829 | 2651 | |
HDM | 14 d at 5 °C vs. Harvest | 477 | 644 |
>21 d at 5 °C vs. Harvest | 2214 | 3065 |
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Olivares, D.; García-Rojas, M.; Ulloa, P.A.; Riveros, A.; Pedreschi, R.; Campos-Vargas, R.; Meneses, C.; Defilippi, B.G. Response Mechanisms of “Hass” Avocado to Sequential 1–methylcyclopropene Applications at Different Maturity Stages during Cold Storage. Plants 2022, 11, 1781. https://doi.org/10.3390/plants11131781
Olivares D, García-Rojas M, Ulloa PA, Riveros A, Pedreschi R, Campos-Vargas R, Meneses C, Defilippi BG. Response Mechanisms of “Hass” Avocado to Sequential 1–methylcyclopropene Applications at Different Maturity Stages during Cold Storage. Plants. 2022; 11(13):1781. https://doi.org/10.3390/plants11131781
Chicago/Turabian StyleOlivares, Daniela, Miguel García-Rojas, Pablo A. Ulloa, Aníbal Riveros, Romina Pedreschi, Reinaldo Campos-Vargas, Claudio Meneses, and Bruno G. Defilippi. 2022. "Response Mechanisms of “Hass” Avocado to Sequential 1–methylcyclopropene Applications at Different Maturity Stages during Cold Storage" Plants 11, no. 13: 1781. https://doi.org/10.3390/plants11131781