A Survey of Enhanced Cold Tolerance and Low-Temperature-Induced Anthocyanin Accumulation in a Novel Zoysia japonica Biotype
Abstract
:1. Introduction
2. Results
2.1. YN-9 Is a Zoysia japonica Biotype
2.2. Enhanced Anthocyanin Accumulation and Cold Tolerance in YN-9
2.3. Read Trimming and Mapping
2.4. DEGs after Cold Treatment
2.5. Genes Enriched in Pigmentation GOs
2.5.1. HD-Zop IV
2.5.2. ABCB Transmembrane Transporter
2.6. Expression Variations of Anthocyanin-Synthesis Genes
2.7. Down-Regulation of Zinc Transporters in YN-9
3. Discussion
3.1. Anthocyanin Accumulation and Cold-Tolerance in YN-9
3.2. Enhanced Expressions of Genes Associated with Anthocyanin Accumulation in YN-9
3.2.1. Anthocyanin-Biosynthesis Genes
3.2.2. HD-IV Transcription Factors
3.3. Enhanced Expressions of Anthocyanin Transport Genes in YN-9
3.4. Down-Regulation of Zinc Transporters Genes in YN-9
3.5. A Proposed Regulation Network of Anthocyanin Accumulation in YN-9
3.6. Future Outlook
4. Materials and Methods
4.1. Plant Materials
4.2. Anthocyanin Assay
4.3. Cold tolerance Assessment
4.4. RNA Extraction, cDNA Library Construction and Illumina Sequencing
4.5. RNA Seq Analysis
4.6. Anthocyanin-Synthesis Gene Profiling
4.7. Phylogenetic Analysis
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Genotype | 2nd Leaf | 3rd Leaf | ||
---|---|---|---|---|
Length (cm) | Width (mm) | Length (cm) | Width (mm) | |
LY-3 | 10.19 ± 1.78 | 4.02 ± 0.55 | 11.93 ± 2.59 | 4.05 ± 0.58 |
YN-9 | 12.47 ± 2.28 ** | 3.92 ± 0.41 | 14.75 ± 2.59 ** | 3.93 ± 0.48 |
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Jin, H.-X.; Jiang, M.; Yang, J.-F.; Wu, Z.-H.; Ma, L.-L.; Wang, C.-C.; Liang, C.; Ning, X.-Y.; Ge, L.-F.; Chen, S. A Survey of Enhanced Cold Tolerance and Low-Temperature-Induced Anthocyanin Accumulation in a Novel Zoysia japonica Biotype. Plants 2022, 11, 429. https://doi.org/10.3390/plants11030429
Jin H-X, Jiang M, Yang J-F, Wu Z-H, Ma L-L, Wang C-C, Liang C, Ning X-Y, Ge L-F, Chen S. A Survey of Enhanced Cold Tolerance and Low-Temperature-Induced Anthocyanin Accumulation in a Novel Zoysia japonica Biotype. Plants. 2022; 11(3):429. https://doi.org/10.3390/plants11030429
Chicago/Turabian StyleJin, Hai-Xiang, Ming Jiang, Jian-Feng Yang, Zhi-Hao Wu, Long-Long Ma, Cong-Cong Wang, Chen Liang, Xin-Yi Ning, Liang-Fa Ge, and Shu Chen. 2022. "A Survey of Enhanced Cold Tolerance and Low-Temperature-Induced Anthocyanin Accumulation in a Novel Zoysia japonica Biotype" Plants 11, no. 3: 429. https://doi.org/10.3390/plants11030429