Exploring the Effects of Different Drying Methods on Related Differential Metabolites of Pleurotus citrinopileatus Singer Based on Untargeted Metabolomics
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fresh Mushroom and Reagents
2.2. Mushroom Preparation
- (1)
- Material selection: When the fruit body color of PCS changed to fresh yellow, and this can also be golden yellow, the fruiting bodies free from blemishes and mechanical damage were collected. And mushrooms of uniform size (including the cap and stipe) and maturity were selected as experimental material.
- (2)
- Mushroom picking: The root of PCS was carefully pulled and picked out. Or a knife was used to cut the roots off at slightly above where the root began.
- (3)
- Washing: The picked fruiting bodies were cleaned with running water to remove remaining attached soil, and then the moisture on the surface was wiped with filter papers.
2.3. Drying Process
2.4. Untargeted Metabolomics Analysis by UHPLC-QE-MS
2.4.1. Sample Preparation and Extraction
2.4.2. LC-MS/MS Conditions
2.4.3. Data Processing and Multivariate Analysis
2.5. Statistical Analysis
3. Results
3.1. Overview of the Metabolic Profiles of PCS Collected from Three Different Drying Methods
3.2. Visualize Analysis of Three Drying Methods of PCS
3.3. Differential Metabolite (DM) Analysis of PCS in Three Different Drying Methods
3.3.1. DMs Profiles in Different Drying Treatments of PCS
3.3.2. Effects of Different Drying Methods on the DMs of PCS
3.4. Metabolic Pathway Analysis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Lu, H.; Peng, S.; Xu, N.; Shang, X.; Liu, J.; Xu, Z.; Jiang, N.; Dong, H.; Wang, R.; Dong, H. Exploring the Effects of Different Drying Methods on Related Differential Metabolites of Pleurotus citrinopileatus Singer Based on Untargeted Metabolomics. Plants 2024, 13, 1594. https://doi.org/10.3390/plants13121594
Lu H, Peng S, Xu N, Shang X, Liu J, Xu Z, Jiang N, Dong H, Wang R, Dong H. Exploring the Effects of Different Drying Methods on Related Differential Metabolites of Pleurotus citrinopileatus Singer Based on Untargeted Metabolomics. Plants. 2024; 13(12):1594. https://doi.org/10.3390/plants13121594
Chicago/Turabian StyleLu, Huan, Simin Peng, Ning Xu, Xiaodong Shang, Jianyu Liu, Zhen Xu, Ning Jiang, Haoran Dong, Ruijuan Wang, and Hui Dong. 2024. "Exploring the Effects of Different Drying Methods on Related Differential Metabolites of Pleurotus citrinopileatus Singer Based on Untargeted Metabolomics" Plants 13, no. 12: 1594. https://doi.org/10.3390/plants13121594