Leaf Membrane Stability under High Temperatures as an Indicator of Heat Tolerance in Potatoes and Genome-Wide Association Studies to Understand the Underlying Genetics
Abstract
:1. Introduction
2. Results
2.1. Extent of Electrolyte Leakage Observed
2.2. REC Significantly Correlated with Tuber Yields, Defects, and Mineral Content
2.3. Genome-Wide Associations
2.4. Heat Shock Protein 18
3. Discussion
4. Materials and Methods
4.1. Electrolyte Leakage Assay
4.1.1. Plant Materials and Growing Conditions
4.1.2. Sample Collection and Heat Treatment
4.1.3. Data Analysis for Electrolyte Leakage Assay
4.2. DNA Extraction and Genotyping
4.3. Genome-Wide Association Studies and Correlation Analysis
4.4. Plant Materials and Growing Conditions for Heat Shock Proteins
4.5. Heat Shock Protein Assessment and Immunoblot Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Traits | Correlation to REC (%) | Probability | Sig. Level |
---|---|---|---|
Total Yield | −0.05 | 0.5710 | ns |
Yield without culls | −0.09 | 0.3200 | ns |
Culls | 0.25 | 0.0042 | ** |
% Tubers with knobs | 0.33 | 0.0001 | *** |
% Tubers with growth cracks | 0.12 | 0.1830 | ns |
% Tubers with external defects | 0.35 | 0.0001 | *** |
% Tubers with hollow heart | 0.15 | 0.0810 | ns |
% Tubers with vascular discoloration | 0.12 | 0.1640 | ns |
% Tubers with internal heat necrosis | 0.27 | 0.0070 | ** |
% Tubers with internal defects | 0.23 | 0.0040 | ** |
Total Yield (Z) | −0.18 | 0.0380 | * |
% Tubers with external defects (Z) | 0.40 | 0.0001 | *** |
% Tubers with internal defects (Z) | 0.34 | 0.0001 | *** |
Weighted multi-index selection (Z) | −0.64 | 0.0001 | *** |
Minerals | Correlation to REC (%) | Probability | Sig. Level |
---|---|---|---|
Nitrogen | −0.21 | 0.0160 | * |
Phosphorus | −0.17 | 0.0550 | ns |
Potassium | −0.08 | 0.3800 | ns |
Calcium | −0.10 | 0.2830 | ns |
Magnesium | −0.25 | 0.0051 | ** |
Sodium | −0.13 | 0.1541 | ns |
Zinc | −0.08 | 0.3492 | ns |
Iron | −0.15 | 0.0843 | ns |
Copper | 0.07 | 0.4391 | ns |
Manganese | −0.22 | 0.0121 | * |
Sulfur | −0. 20 | 0.0230 | * |
Boron | −0.17 | 0.0630 | ns |
Weighted multi-index selection (Z) | −0.25 | 0.0041 | ** |
Model | SNP at QTL Peak | Chr | Peak Position (bp) | Score 1 − log10(p) | R2 (%) |
---|---|---|---|---|---|
Additive | PotVar0071937 | 1 | 1,158,384 | 5.9 | 2.60 |
Additive | solcap_snp_c2_30963 | 1 | 88,362,869 | 7.3 | 1.40 |
Additive | PotVar0056569 | 3 | 50,880,446 | 18.5 | 4.30 |
Additive | PotVar0020249 | 3 | 61,550,994 | 13.1 | 1.50 |
Additive | solcap_snp_c2_55774 | 4 | 65,972,478 | 6.8 | 0.01 |
Additive | solcap_snp_c2_48581 | 5 | 39,349,874 | 6.0 | 1.50 |
Additive | solcap_snp_c2_2034 | 7 | 37,157,836 | 11.3 | 3.90 |
Additive | solcap_snp_c1_7381 | 7 | 42,659,912 | 8.4 | 1.20 |
Additive | solcap_snp_c2_57107 | 11 | 10,505,722 | 5.8 | 0.05 |
1-dom-ref | PotVar0056569 | 3 | 50,880,446 | 7.1 | 8.20 |
1-dom-ref | solcap_snp_c1_7381 | 7 | 42,659,912 | 16.6 | 13.80 |
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Ifeduba, A.M.; Zhen, S.; Pandey, J.; Vales, M.I. Leaf Membrane Stability under High Temperatures as an Indicator of Heat Tolerance in Potatoes and Genome-Wide Association Studies to Understand the Underlying Genetics. Plants 2024, 13, 2175. https://doi.org/10.3390/plants13162175
Ifeduba AM, Zhen S, Pandey J, Vales MI. Leaf Membrane Stability under High Temperatures as an Indicator of Heat Tolerance in Potatoes and Genome-Wide Association Studies to Understand the Underlying Genetics. Plants. 2024; 13(16):2175. https://doi.org/10.3390/plants13162175
Chicago/Turabian StyleIfeduba, Amaka M., Shuyang Zhen, Jeewan Pandey, and M. Isabel Vales. 2024. "Leaf Membrane Stability under High Temperatures as an Indicator of Heat Tolerance in Potatoes and Genome-Wide Association Studies to Understand the Underlying Genetics" Plants 13, no. 16: 2175. https://doi.org/10.3390/plants13162175