An Effective Somatic-Cell Regeneration and Genetic Transformation Method Mediated by Agrobacterium tumefaciens for Portulaca oleracea L.
Abstract
:1. Introduction
2. Results
2.1. Callus Induction of Different Parts of Purslane and Medium Optimization
2.2. Shoot Regeneration and Medium Optimization
2.3. The Developmental Directions of Leaf Explants Vary on Different Media
2.4. Sensitivity of Seedlings and Explants to Antibiotics
2.5. Genetic Transformation Workflow Mediated by Agrobacterium tumefaciens
2.6. Transgenic Plant Identification
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Species Identification
4.2. Sterilization and Aseptic Seedling Preparation
4.3. Callus Induction and Somatic-Cell Regeneration
4.4. Identification of the Sensitivity to Different Antibiotics
4.5. Agrobacterium tumefaciens Culture and Plant Transformation
4.6. GUS Staining and Polymerase Chain Reaction (PCR) Identification
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Accession No. | City | Province | District | Shoot Regeneration |
---|---|---|---|---|
0# | Guangzhou | Guangdong | South China | Yes |
8# | Dehong | Yunnan | Southwest China | Yes |
10# | Baise | Guangxi | South China | Yes |
17# | Jiulongpo | Chongqing | Southwest China | Yes |
21# | Shangqiu | Henan | Central China | Yes |
33# | Xi’an | Shanxi | North China | Yes |
38# | Guiping | Guangxi | South China | Yes |
43# | Jiamusi | Heilongjiang | Northeast China | Yes |
46# | Yulin | Guangxi | South China | Yes |
47# | Yingtan | Jiangxi | East China | Yes |
62# | Zhoukou | Henan | Central China | Yes |
64# | Zhenghzhou | Henan | Central China | Yes |
65# | Ledong | Hainan | South China | Yes |
78# | Datong | Shanxi | North China | Yes |
102# | Hechuan | Chongqing | Southwest China | Yes |
106# | Zhanjiang | Guangdong | South China | Yes |
115# | Suihua | Heilongjiang | Northeast China | Yes |
116# | Huainan | Anhui | East China | Yes |
117# | Liuyang | Hunan | Central China | Yes |
118# | Liuyang | Hunan | Central China | No |
119# | Suzhou | Anhui | East China | Yes |
320# | Baicheng | Jilin | Northeast China | Yes |
326# | Zhoukou | Henan | Central China | Yes |
313# | Yuxi | Yunan | Southwest China | Yes |
325# | Hefei | Anhui | East China | Yes |
500# | Guangzhou | Guangdong | South China | Yes |
ZP# | Huai’an | Jiangsu | East China | Yes |
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Xu, M.; Zhao, X.; Fang, J.; Yang, Q.; Li, P.; Yan, J. An Effective Somatic-Cell Regeneration and Genetic Transformation Method Mediated by Agrobacterium tumefaciens for Portulaca oleracea L. Plants 2024, 13, 2390. https://doi.org/10.3390/plants13172390
Xu M, Zhao X, Fang J, Yang Q, Li P, Yan J. An Effective Somatic-Cell Regeneration and Genetic Transformation Method Mediated by Agrobacterium tumefaciens for Portulaca oleracea L. Plants. 2024; 13(17):2390. https://doi.org/10.3390/plants13172390
Chicago/Turabian StyleXu, Mengyun, Xinyu Zhao, Jiahui Fang, Qinwen Yang, Ping Li, and Jian Yan. 2024. "An Effective Somatic-Cell Regeneration and Genetic Transformation Method Mediated by Agrobacterium tumefaciens for Portulaca oleracea L." Plants 13, no. 17: 2390. https://doi.org/10.3390/plants13172390