LrHSP17.2 Plays an Important Role in Abiotic Stress Responses by Regulating ROS Scavenging and Stress-Related Genes in Lilium regale
Abstract
:1. Introduction
2. Results
2.1. LrHSP17.2 Is a Heat-Inducible Heat Shock Protein Member of Lilium regale
2.2. LrHSP17.2 Localizes to the Cytoplasm and Accumulates Near the Nucleus
2.3. Overexpression of LrHSP17.2 Promote Root Growth at Seedling Stages
2.4. Overexpression of LrHSP17.2 Enhances the Thermotolerance of Transgenic Plants by ROS Pathways
2.5. Promoter Cloning and Analysis of LrHSP17.2
2.6. LrHSP17.2 Is Involved in Drought and Salt Stress Regulation in Transgenic Tobacco Plants
2.7. LrHSP17.2 Is Induced by ABA and MT and Participates in Their Synthetic Pathways
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Molecular Cloning and Sequence Analysis of LrHSP17.2
4.3. Subcellular Localization of LrHSP17.2
4.4. Heat Stress Treatment of Lily
4.5. Gene Expression Analysis in Lily
4.6. Isolation and Analysis of Promoter Sequences
4.7. Plant Transformation and Generation of Transgenic Lines
4.8. Abiotic Stress Treatment of Tobacco
4.9. Physiological Assays
4.10. Exogenous Hormone Treatment
4.11. Related Gene Expression in Transgenic Plants
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Element | Core | Function | Number |
---|---|---|---|
ABRE | ACGTG | ABA response | 1 |
ARE | AAACCA | Anaerobic-induced regulation | 3 |
CGTCA-motif | CGTCA | MeJA response | 2 |
G-BOX | CACGTT | Light response | 1 |
LTR | CCGAAA | Low temperature response | 1 |
MYB | C/TAACCA | MYB transcription factor binding | 9 |
TC-rich repeats | GTTTTCTTAC | defense and stress response | 1 |
W-BOX | TGAC | WRKY transcription factor binding | 10 |
CCAAT-BOX | CCAAT | related to plant heat tolerance | 7 |
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Tang, S.; Ling, Q.; Ma, Q.; Cheng, Y.; Mei, P.; Miao, Y.; Pan, Y.; Jia, Y.; Wu, M.; Yong, X.; et al. LrHSP17.2 Plays an Important Role in Abiotic Stress Responses by Regulating ROS Scavenging and Stress-Related Genes in Lilium regale. Plants 2024, 13, 2416. https://doi.org/10.3390/plants13172416
Tang S, Ling Q, Ma Q, Cheng Y, Mei P, Miao Y, Pan Y, Jia Y, Wu M, Yong X, et al. LrHSP17.2 Plays an Important Role in Abiotic Stress Responses by Regulating ROS Scavenging and Stress-Related Genes in Lilium regale. Plants. 2024; 13(17):2416. https://doi.org/10.3390/plants13172416
Chicago/Turabian StyleTang, Shaokang, Qin Ling, Qiqi Ma, Yuqing Cheng, Peng Mei, Yuan Miao, Yuanzhi Pan, Yin Jia, Mengxi Wu, Xue Yong, and et al. 2024. "LrHSP17.2 Plays an Important Role in Abiotic Stress Responses by Regulating ROS Scavenging and Stress-Related Genes in Lilium regale" Plants 13, no. 17: 2416. https://doi.org/10.3390/plants13172416