Effects of Exogenous Abscisic Acid on the Physiological and Biochemical Responses of Camellia oleifera Seedlings under Drought Stress
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Materials and Design
2.2. Measurement of Photosynthetic Parameters
2.3. Determination of Physiological and Biochemical Indices
2.4. Membership Function Method
2.5. RNA Extraction and Real-Time Fluorescent Quantitative Analysis
2.6. Statistical Analysis
3. Results
3.1. Effects of Exogenous ABA on Photosynthetic Parameters of C. oleifera Seedlings under Drought Stress
3.2. Effects of Exogenous ABA on Physiological Indicators of C. oleifera Seedlings under Drought Stress
3.3. Effects of Exogenous ABA on Gene Expression in C. oleifera Seedlings under Drought Stress
3.4. Comprehensive Evaluation through Membership Function Analysis
4. Discussion
4.1. Photosynthetic Parameters
4.2. Physiological Indicators
4.3. Gene Expression
4.4. Limitations and Future Prospects
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Gene | Forward Primer (5′-3′) | Reverse Primer (5′-3′) |
---|---|---|
CoTubulin | CCATGCCTTGGATCACATTT | TGGGGCCATTAATGTAGACG |
CoPP2C16 | GGAGGCAAGGTGATTCAGTGG | GGCATTCGTCTTCTTTCGTTCG |
CoPP2C51 | CAGGGTGTTTGGTGTTCTTTCC | CTCATCCTCGTCACTCCTTGTC |
CoPP2C75 | TTCTCGGTGTTCTCGCTATGTC | GTCGCTCGCCAGTATCAGG |
CoSNRK2.8 | GCATTCCCAACCCAAATCTACAG | GCACCAACCAACATCACATATAAGG |
CoTOC1 | AGGAGAAGGCGAATGCTTGG | CTCTACAGGAGCAGCAGCAG |
CoDMR6 | TCAAGGATGGCAAGTGGATGG | GGCAGAGGAAAGAGGCTATGG |
CoaccA | CGAAACCGGTCTGGACTTCA | CTCGATCTCCGTGAAGCTCC |
CoaccB | CCACACCACCACCTATTCCC | TCCAGAAGCCTCCAATGCTG |
CoGK | AGTTGAGTCCACTGGCGGAGTT | ACAGCACGAGCGATGTGAGACT |
CoFAD6 | GGCTCAGCTCAATGGCACAGTT | CGCCAATTCCAAGTCGCCTCAT |
CoFAD7 | ATCATGGCATCCGTTGTCTGA | GGTCTTCCCAGGACTTCTACCC |
CoFAD8 | TCAATGGCGTCAATGGGG | CGGAATCGCAGCTCGAATAT |
Group | Membership Function Value | D | Rank | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Pn | Gs | Tr | Ci | SOD | POD | MDA | Pro | GSH | SP | SS | |||
A0 | 1 | 0.67 | 0.24 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.17 | 4 |
A50 | 0.54 | 0.41 | 0.61 | 0.29 | 1 | 1 | 0.66 | 1 | 1 | 1 | 0.59 | 0.73 | 1 |
A100 | 0.87 | 1 | 0 | 0.8 | 0.59 | 0.52 | 0.19 | 0.71 | 0.75 | 0.88 | 1 | 0.66 | 2 |
A200 | 0 | 0 | 1 | 1 | 0.31 | 0.54 | 1 | 0.18 | 0.68 | 0.27 | 0.58 | 0.51 | 3 |
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Yang, D.; Chen, Y.; Wang, R.; He, Y.; Ma, X.; Shen, J.; He, Z.; Lai, H. Effects of Exogenous Abscisic Acid on the Physiological and Biochemical Responses of Camellia oleifera Seedlings under Drought Stress. Plants 2024, 13, 225. https://doi.org/10.3390/plants13020225
Yang D, Chen Y, Wang R, He Y, Ma X, Shen J, He Z, Lai H. Effects of Exogenous Abscisic Acid on the Physiological and Biochemical Responses of Camellia oleifera Seedlings under Drought Stress. Plants. 2024; 13(2):225. https://doi.org/10.3390/plants13020225
Chicago/Turabian StyleYang, Dayu, Yongzhong Chen, Rui Wang, Yimin He, Xiaofan Ma, Jiancai Shen, Zhilong He, and Hanggui Lai. 2024. "Effects of Exogenous Abscisic Acid on the Physiological and Biochemical Responses of Camellia oleifera Seedlings under Drought Stress" Plants 13, no. 2: 225. https://doi.org/10.3390/plants13020225