Genome-Wide Identification of Osmanthus fragrans Histone Modification Genes and Analysis of Their Expression during the Flowering Process and under Azacytidine and Ethylene Treatments
Abstract
:1. Introduction
2. Results
2.1. Identification and Characterization Analysis of HMs in the O. fragrans Genome
2.2. Phylogenetic Analysis of HMs between O. fragrans and Arabidopsis
2.3. Gene Structure and Conserved Motif Analyses of OfHMs
2.4. Cis-Acting Element Analysis of OfHMs
2.5. Chromosomal Distribution and Synteny Analysis of OfHMs
2.6. Functional Enrichment Analysis of OfHMs
2.7. Prediction of Interactions of OfHM Proteins
2.8. Expression Analysis of OfHMs in Different Tissues and Flowering Stages
2.9. Expression Analysis of OfHMs under Ethylene and 5′-Azacytidine (Aza) Treatment
2.10. Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) Analysis of OfHM Genes
3. Discussion
3.1. O. fragrans HM Genes in Comparison with Other Plant Species
3.2. Evolution and Amplification Analyses of OfHM Gene Family
3.3. OfHMs Are Involved in the Flowering Process and the Induction of Aza and Ethylene Responses
4. Materials and Methods
4.1. Identification and Chromosome Location of OfHM Gene Family
4.2. Physicochemical Characteristics Examination, Phylogenetic Tree Elaboration, and Gene Structure Research
4.3. Cis-Acting Regulatory Element Analysis of OfHM Genes
4.4. Tandem Duplication and Synteny Analyses
4.5. Protein–Protein Interaction Network Construction and Functional Enrichment Analysis of OfHM Genes
4.6. Plant Materials and Treatment
4.7. Identification of OfHM Expression Profiles Using High-Throughput Sequencing
4.8. qRT-PCR Analysis of OfHM Genes
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Xia, H.; Zhang, Y.; Chen, X.; Zeng, X.; Cai, X.; Li, Z.; Chen, H.; Yang, J.; Zou, J. Genome-Wide Identification of Osmanthus fragrans Histone Modification Genes and Analysis of Their Expression during the Flowering Process and under Azacytidine and Ethylene Treatments. Plants 2024, 13, 777. https://doi.org/10.3390/plants13060777
Xia H, Zhang Y, Chen X, Zeng X, Cai X, Li Z, Chen H, Yang J, Zou J. Genome-Wide Identification of Osmanthus fragrans Histone Modification Genes and Analysis of Their Expression during the Flowering Process and under Azacytidine and Ethylene Treatments. Plants. 2024; 13(6):777. https://doi.org/10.3390/plants13060777
Chicago/Turabian StyleXia, Hui, Yingting Zhang, Xiang Chen, Xiangling Zeng, Xuan Cai, Zeqing Li, Hongguo Chen, Jie Yang, and Jingjing Zou. 2024. "Genome-Wide Identification of Osmanthus fragrans Histone Modification Genes and Analysis of Their Expression during the Flowering Process and under Azacytidine and Ethylene Treatments" Plants 13, no. 6: 777. https://doi.org/10.3390/plants13060777