Plants

26 pages, 12486 KB

Open AccessArticle

Diversity Patterns of Spontaneous Plants and Their Multi-Scale Driving Mechanisms in Cold Regions: A Case of 14 Cities in Heilongjiang Province, China

by Feinuo Li, Congcong Zhao, Haiyan Zhu, Xueting Yang and Yuandong Hu

Plants 2025, 14(20), 3145; https://doi.org/10.3390/plants14203145 (registering DOI) - 12 Oct 2025

Abstract

Cold-climate cities remain poorly studied, yet their spontaneous flora is strongly shaped by severe winters and short growing seasons. Heilongjiang Province, the northernmost region of China, provides a valuable case study given its rapid urbanization. As an important component of urban biodiversity, the [...] Read more.

Cold-climate cities remain poorly studied, yet their spontaneous flora is strongly shaped by severe winters and short growing seasons. Heilongjiang Province, the northernmost region of China, provides a valuable case study given its rapid urbanization. As an important component of urban biodiversity, the diversity distribution patterns of spontaneous plants and their underlying causes remain underexplored from multi-scale and multi-dimensional perspectives. Therefore, this study aimed to test how climatic subzones and habitat types jointly influence spontaneous plant diversity across urban landscapes in 14 cities of Heilongjiang Province. Based on vegetation surveys, we applied calculations of α- and β-diversity, along with hierarchical clustering, across climatic subzones, cities, and habitat types to elucidate the diversity patterns and their multi-scale driving mechanisms. The results showed the following: (1) A total of 778 spontaneous plant species were recorded, belonging to 98 families and 395 genera. Native plants accounted for 58.7%, and non-native plants accounted for 41.3% (including 77 invasive species). (2) Perennial herbs dominated overall (45.2%), consistent with winter filtering, whereas annual/biennial herbs were more common in warmer subzones such as II B2. (3) Forest gaps (FG) and shrub–grassland gaps (SG) supported the most diverse spontaneous plant communities, highlighting habitat heterogeneity. (4) Species richness peaked in subzone II B2 and was lowest in subzone I A1, while abandoned land (SA) and shrub–grassland gaps (SG) supported the richest communities. (5) β-diversity analyses indicated homogenization under extreme cold in subzone I A1 and greater turnover in warmer subzone II B2, reflecting contrasting climatic filters. The “light patches” in FG habitats and the “disturbance filtering” in LA habitats further shaped the differences in local communities. This study reveals the diversity distribution patterns and adaptation strategies of spontaneous plants in cold cities, emphasizing their integration into urban planning while addressing the dominance of invasive species. Full article

(This article belongs to the Section Plant Ecology)

20 pages, 1326 KB

Open AccessArticle

Effects of Canopy Litter Removal on Canopy Structure, Understory Light and Vegetation Dynamics in Cunninghamia lanceolata Plantations of Varying Densities

by Lili Zhou, Lixian Zhang, Qi Liu, Yulong Chen, Zongming He, Shubin Li and Xiangqing Ma

Plants 2025, 14(20), 3144; https://doi.org/10.3390/plants14203144 (registering DOI) - 12 Oct 2025

Abstract

The prolonged retention of senescent branches and needles (canopy litter) in Cunninghamia lanceolata canopies is an evolutionary adaptation, yet its impacts on stand microenvironment and understory succession remain poorly quantified. To address this gap, we conducted a 5-year field experiment across six planting [...] Read more.

The prolonged retention of senescent branches and needles (canopy litter) in Cunninghamia lanceolata canopies is an evolutionary adaptation, yet its impacts on stand microenvironment and understory succession remain poorly quantified. To address this gap, we conducted a 5-year field experiment across six planting densities (1800, 2400, 3000, 3600, 4200, and 4800 trees·ha⁻¹), aiming to evaluate the effects of canopy litter removal on canopy structure, forest light environment, and understory biodiversity. Results demonstrated that leaf area index (LAI) and mean tilt angle of the leaf (MTA) significantly increased with density (p < 0.05), leading to marked reductions in photosynthetic photon flux density (PPFD) and light transmittance (T). Canopy litter removal significantly reduced LAI across all densities after 4–5 years (p < 0.05) and consistently enhanced PPFD and transmittance (p < 0.01). MTA and light quality parameters (red:blue and red:far-red ratios) both exhibited variable responses to litter removal, driven by density and time interactions, with effects diminishing over time. Understory vegetation diversity exhibited pronounced temporal dynamics and density-dependent responses to canopy litter removal, with increases in species richness (S), Simpson diversity (D), and Shannon–Wiener diversity (H), while Pielou Evenness (J) responded more variably. The most notable increase in species richness occurred in the 4th year, when 21 new species were recorded, largely due to the expansion of light-demanding bamboos (e.g., Indocalamus tessellatus and Pleioblastus amarus), heliophilic grasses (e.g., Lophatherum gracile) and pioneer ferns (e.g., Pteris dispar and Microlepia hancei). Correlation analyses confirmed PPFD as a key positive driver of all diversity indices (p < 0.01), whereas LAI was significantly negatively correlated with PPFD, light transmittance, and understory diversity (p < 0.01). These findings demonstrate that strategic management of canopy litter incorporating stand density regulation can improve understory light availability, thereby facilitating heliophilic species recruitment and biodiversity enhancement in subtropical coniferous plantations. Full article

(This article belongs to the Special Issue Climate Change and Metal Stress on Plants: Potential Impacts and Survival Strategies)

20 pages, 4272 KB

Open AccessArticle

Transcription Factor Analysis of Rhodophytes Suggests Trihelix Transcription Factors Across the Florideophyceae

by Lachlan J. McKinnie, Scott F. Cummins, Sankar Subramanian and Min Zhao

Plants 2025, 14(20), 3143; https://doi.org/10.3390/plants14203143 (registering DOI) - 12 Oct 2025

Abstract

Transcription factors (TFs) are important gene transcription regulators involved in myriad functions such as development, metabolism, and stress response. TFs are found in all eukaryotes, with many families of TFs unique to plants and algae. Algae are of interest due to a wide [...] Read more.

Transcription factors (TFs) are important gene transcription regulators involved in myriad functions such as development, metabolism, and stress response. TFs are found in all eukaryotes, with many families of TFs unique to plants and algae. Algae are of interest due to a wide range of novel metabolites, of which TFs play an important role in regulating their biosynthesis. In particular, the red algae (phylum Rhodophyta) are a source of several important metabolites that are a current focus of further research. However, to date, investigations of TF families in rhodophytes have been limited due to the relative lack of genomic resources available and the small number of in silico analyses of their TFs. In this study, we used genomic and transcriptomic data to identify rhodophyte TFs. We found that the general proportion of TFs in rhodophytes was overall consistent with previous research. However, for the first time in the rhodophyte class Florideophyceae, we report the presence of a putative TF within the trihelix TF (TTF) family, which are light-sensitive TFs associated with growth and stress response. In particular, we demonstrate evidence suggesting the presence of putative TTFs in three Asparagopsis taxiformis genomes, as well as in several other florideophyte assemblies. This was supported by analyses including Neighbour-Joining phylogeny, protein structure prediction, and motif analysis. In summary, this research reported the repertoire of TFs in rhodophyte algae across a much greater range than previously reported and identified putative TTFs in several algae from the class Florideophyceae. This opens an avenue for further research into the evolution of various TFs in early plants, as well as key regulatory factors in rhodophyte metabolism, though future research, such as functional characterisation, will be required to confirm these findings. Full article

(This article belongs to the Section Plant Molecular Biology)

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16 pages, 6994 KB

Open AccessArticle

Physiological Responses of Grapevine Leaves to High Temperature at Different Senescence Periods

by Shiwei Guo, Riziwangguli Abudureheman, Zekai Zhang, Haixia Zhong, Fuchun Zhang, Xiping Wang, Mansur Nasir and Jiuyun Wu

Plants 2025, 14(20), 3142; https://doi.org/10.3390/plants14203142 (registering DOI) - 12 Oct 2025

Abstract

Leaf senescence is a precisely regulated developmental process that is critical for grapevine growth and yield, which is easily influenced by environmental factors. High temperature is a major factor that accelerates senescence rapidly, adversely affects photosynthetic performance, severely hindering fruit nutrient metabolism and [...] Read more.

Leaf senescence is a precisely regulated developmental process that is critical for grapevine growth and yield, which is easily influenced by environmental factors. High temperature is a major factor that accelerates senescence rapidly, adversely affects photosynthetic performance, severely hindering fruit nutrient metabolism and growth. This study investigated chlorophyll fluorescence and physiological traits in grape (Vitis vinifera L.) leaves at different senescence stages under natural high-temperature conditions in Turpan. Measurements included chlorophyll content, MDA levels, antioxidant enzyme activities, and chlorophyll fluorescence parameters. The results showed that (1) young leaves exhibited higher and more sustained chlorophyll content but were prone to wilting, whereas older leaves showed accelerated chlorosis and functional decline; (2) high temperature severely impaired PSII function, inhibiting electron transport and photochemical efficiency, reflected in increased ABS/RC, TR_o/RCC, and DI_o/RC, and decreased F_v/F_m, F_v/F_o, and PI_abs; (3) POD, SOD, CAT and MDA levels initially increased then decreased, correlating with photosynthetic changes and leaf age; and (4) young leaves maintained stronger photosynthetic capability and physiological resilience than older ones. Although partial recovery occurred after temperature reduction, photosynthetic and antioxidant activities did not fully revert. This suggests persistent heat-induced functional decline and accelerated senescence, providing insights for understanding heat-induced leaf senescence and developing strategies for cultivating grapevines. Full article

(This article belongs to the Section Horticultural Science and Ornamental Plants)

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17 pages, 1462 KB

Open AccessArticle

Seed Germination Ecology and Longevity of the Invasive Aquatic Plant Sagittaria platyphylla

by Nguyen Nguyen, Tobias Bickel, Sundaravelpandian Kalaipandian and Steve Adkins

Plants 2025, 14(20), 3138; https://doi.org/10.3390/plants14203138 (registering DOI) - 12 Oct 2025

Abstract

Sagittaria platyphylla (Engelm.) J.G.Sm. is an invasive aquatic plant of concern in Australian freshwater systems. Understanding its seed germination ecology and seedbank longevity is critical for effective management. This study examined environmental influences on germination and longevity through three controlled experiments. Seeds germinated [...] Read more.

Sagittaria platyphylla (Engelm.) J.G.Sm. is an invasive aquatic plant of concern in Australian freshwater systems. Understanding its seed germination ecology and seedbank longevity is critical for effective management. This study examined environmental influences on germination and longevity through three controlled experiments. Seeds germinated between 17 and 29 °C, with optimal germination (96 ± 2%) at 21 °C under a 12/12 h light/dark photoperiod. High germination (93–99%) also occurred under light in diurnal regimes of 15/5 °C, 25/15 °C, and 30/20 °C. In a burial experiment, seedlings emerged only from surface-sown seeds (76 ± 4%); no emergence occurred from buried seeds, though viability remained high, peaking at 98 ± 2% at 2.5 cm depth. A controlled aging test indicated a 50% viability loss (P₅₀) in 36 days under warm, moist laboratory conditions. Based on established criteria, S. platyphylla produces short-lived seeds, which are likely to persist in the substrate seedbank for <1 to 3 years. The strong light dependence of germination suggests that sediment disturbance, which exposes buried seeds to light, could significantly enhance recruitment, highlighting the importance of minimizing disturbance for effective long-term management. Full article

(This article belongs to the Special Issue Ecology and Management of Invasive Plants—2nd Edition)

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15 pages, 2736 KB

Open AccessArticle

Exploring the Hyperspectral Response of Quercetin in Anoectochilus roxburghii (Wall.) Lindl. Using Standard Fingerprints and Band-Specific Feature Analysis

by Ziyuan Liu, Haoyuan Ding, Sijia Zhao, Hongzhen Wang and Yiqing Xu

Plants 2025, 14(20), 3141; https://doi.org/10.3390/plants14203141 (registering DOI) - 11 Oct 2025

Abstract

Quercetin, a key flavonoid in Anoectochilus roxburghii (Wall.) Lindl., plays an important role in determining the pharmacological value of this medicinal herb. However, traditional methods for quercetin quantification are destructive and time-consuming, limiting their application in real-time quality monitoring. This study investigates the [...] Read more.

Quercetin, a key flavonoid in Anoectochilus roxburghii (Wall.) Lindl., plays an important role in determining the pharmacological value of this medicinal herb. However, traditional methods for quercetin quantification are destructive and time-consuming, limiting their application in real-time quality monitoring. This study investigates the hyperspectral response characteristics of quercetin using near-infrared hyperspectral imaging and establishes a feature-based model to explore its detectability in A. roxburghii leaves. We scanned standard quercetin solutions of known concentration under the same imaging conditions as the leaves to produce a dilution series. Feature-selection methods used included the successive projections algorithm (SPA), Pearson correlation, and competitive adaptive reweighted sampling (CARS). A 1D convolutional neural network (1D-CNN) trained on SPA-selected wavelengths yielded the best prediction performance. These key wavelengths—particularly the 923 nm band—showed strong theoretical and statistical relevance to quercetin’s molecular absorption. When applied to plant leaf spectra, the standard-trained model produced continuous predicted quercetin values that effectively distinguished cultivars with varying flavonoid contents. PCA visualization and ROC-based classification confirmed spectral transferability and potential for functional evaluation. This study demonstrates a non-destructive, spatially resolved, and biochemically interpretable strategy for identifying bioactive markers in plant tissues, offering a methodological basis for future hyperspectral inversion studies and intelligent quality assessment in herbal medicine. Full article

(This article belongs to the Special Issue Application of Hyperspectral Remote Sensing Technology in Plant Research)

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23 pages, 18619 KB

Open AccessArticle

Comprehensive Identification and Expression Analysis of the SWEET Gene Family in Actinidia eriantha Reveals That Two AeSWEET11 Genes Function in Sucrose and Hexose Transport

by Xin Feng, Qingqing Huang, Minxia Gao, Ruilian Lai and Yiting Chen

Plants 2025, 14(20), 3140; https://doi.org/10.3390/plants14203140 (registering DOI) - 11 Oct 2025

Abstract

Sugars are key metabolites influencing the flavor and quality of kiwifruit, with their accumulation in fruit relying on sugar transporters. Recently identified sugar transporters known as SWEETs play significant roles in modulating plant growth, development, and fruit ripening. However, the characteristics of SWEET [...] Read more.

Sugars are key metabolites influencing the flavor and quality of kiwifruit, with their accumulation in fruit relying on sugar transporters. Recently identified sugar transporters known as SWEETs play significant roles in modulating plant growth, development, and fruit ripening. However, the characteristics of SWEET genes in Actinidia eriantha remain poorly understood. In this study, a total of 26 AeSWEET genes were identified across 17 chromosomes. These genes encoded proteins ranging from 198 to 305 amino acids in length and contained 5 to 7 transmembrane helices. Both interspecific and intraspecific phylogenetic trees categorized AeSWEET proteins into four distinct clades. The motif and domain structures were conserved within each clade, although variations were observed in exon-intron organizations. One tandem and fourteen segmental duplication events were identified as primary drivers of the AeSWEET family expansion. Comparative syntenic mapping showed a closer homology of the AeSWEET family with that of dicotyledons compared to monocotyledons. Promoter cis-element analysis indicated the potential responses of AeSWEET genes to five phytohormones and seven environmental stressors. Quantitative real-time PCR analysis revealed tissue-specific expression profiles of AeSWEET genes, with two AeSWEET11 genes (AeSWEET11a and AeSWEET11b) showing significantly higher expression levels in fruit tissues. Their expressions were positively correlated with sucrose, fructose, and glucose contents throughout fruit development and ripening. Transient transformation tests in tobacco leaves verified the predominant localization of AeSWEET11a and AeSWEET11b to the plasma membrane. Functional assays in yeast mutants revealed that AeSWEET11a and AeSWEET11b both possessed sucrose and hexose transport activities. These findings highlight the potential of targeting AeSWEET11a and AeSWEET11b to enhance sugar accumulation in the fruit of A. eriantha, thereby providing a foundation for improving the flavor profile of commercial cultivars. Full article

(This article belongs to the Special Issue Quality Improvement of Fruit Trees: Integrating Omics and Cultivation Techniques)

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20 pages, 2684 KB

Open AccessArticle

Genome-Wide Identification and Expression Analysis of the SRS Gene Family in Hylocereus undatus

by Fanjin Peng, Lirong Zhou, Shuzhang Liu, Renzhi Huang, Guangzhao Xu and Zhuanying Yang

Plants 2025, 14(20), 3139; https://doi.org/10.3390/plants14203139 (registering DOI) - 11 Oct 2025

Abstract

SHORT INTERNODE (SHI)-Related Sequence (SRS) transcription factors play crucial roles in plant growth, development, and stress responses and have been extensively studied in various plant species. However, the molecular functions and regulatory mechanisms of SRS genes in the economically important tropical fruit crop [...] Read more.

SHORT INTERNODE (SHI)-Related Sequence (SRS) transcription factors play crucial roles in plant growth, development, and stress responses and have been extensively studied in various plant species. However, the molecular functions and regulatory mechanisms of SRS genes in the economically important tropical fruit crop pitaya (Hylocereus undatus) remain poorly understood. This study identified 9 HuSRS genes in pitaya via bioinformatics analysis, with subcellular localization predicting nuclear distributions for all. Gene structure analysis showed 1–4 exons, and conserved motifs (RING-type zinc finger and IXGH domains) were shared across subclasses. Phylogenetic analysis classified the HuSRS genes into three subfamilies. Subfamily I (HuSRS1–HuSRS4) is closely related to poplar and tomato homologs and subfamily III (HuSRS6–HuSRS8) contains a recently duplicated paralogous pair (HuSRS7/HuSRS8) and shows affinity to rice SRS genes. Protein structure prediction revealed dominance of random coils, α-helices, and extended strands, with spatial similarity correlating to subfamily classification. Interaction networks showed HuSRS1, HuSRS2, HuSRS7 and HuSRS8 interact with functional proteins in transcription and hormone signaling. Promoter analysis identified abundant light/hormone/stress-responsive elements, with HuSRS5 harboring the most motifs. Transcriptome and qPCR analyses revealed spatiotemporal expression patterns: HuSRS4, HuSRS5, and HuSRS7 exhibited significantly higher expression levels in callus (WG), which may be associated with dedifferentiation capacity. In seedlings, HuSRS9 exhibited extremely high transcriptional accumulation in stem segments, while HuSRS1, HuSRS5, HuSRS7 and HuSRS8 were highly active in cotyledons. This study systematically analyzed the characteristics of the SRS gene family in pitaya, revealing its evolutionary conservation and spatio-temporal expression differences. The research results have laid a foundation for in-depth exploration of the function of the SRS gene in the tissue culture and molecular breeding of pitaya. Full article

(This article belongs to the Section Plant Genetics, Genomics and Biotechnology)

13 pages, 3937 KB

Open AccessArticle

Evaluation of Leguminous Plants as Phytoremediator Species in Soil with Pesticide and Vinasse Interactions

by Munick Beato Aragão, Emanuella Roberto Ribeiro, Yanca Araujo Frias, Victor Hugo Cruz, Thalia Silva Valério, Alexandre Ribeiro Batista, Paulo Henrique Frata Ferreira, Henzo Henrique Simionatto and Paulo Renato Matos Lopes

Plants 2025, 14(20), 3137; https://doi.org/10.3390/plants14203137 (registering DOI) - 11 Oct 2025

Abstract

Sugarcane is a key crop for sugar, biofuels, and bioenergy, with Brazil as the world’s largest producer. Intensive cultivation demands pesticides like tebuthiuron and thiamethoxam, while fertigation with vinasse may alter their environmental behavior. Sustainable approaches, such as phytoremediation, are needed to mitigate [...] Read more.

Sugarcane is a key crop for sugar, biofuels, and bioenergy, with Brazil as the world’s largest producer. Intensive cultivation demands pesticides like tebuthiuron and thiamethoxam, while fertigation with vinasse may alter their environmental behavior. Sustainable approaches, such as phytoremediation, are needed to mitigate negative impacts on soil quality. This study assessed the phytoremediation potential of Canavalia ensiformis and Mucuna pruriens in soils contaminated with tebuthiuron, thiamethoxam, and vinasse under greenhouse conditions. Experiments used a completely randomized design (five replicates, 4 × 2 factorial). Plant development impacts on the sentinel species Crotalaria juncea, and ecotoxicity via Lactuca sativa bioassays were evaluated. Tebuthiuron strongly inhibited C. ensiformis, while thiamethoxam showed mild stimulatory effects. M. pruriens maintained better growth in the presence of contaminants. Bioassays revealed greater residual toxicity in tebuthiuron treatments. Overall, M. pruriens demonstrated superior biomass production and capacity to lessen soil toxicity, underscoring its potential as a sustainable tool for phytoremediation of pesticide-impacted soils. Full article

(This article belongs to the Special Issue Advances in Phytoremediation Strategies to Address Global Environmental Challenges)

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19 pages, 2038 KB

Open AccessArticle

Synergistic Remediation of Eutrophic Rural Pond Water Using Submerged Macrophytes and Daphnia magna

by Haoyu Cao, Chunxue Zhang, Bo Yang, Liyuan Liu, Jiarui Wang and Xiangqun Zheng

Plants 2025, 14(20), 3136; https://doi.org/10.3390/plants14203136 (registering DOI) - 11 Oct 2025

Abstract

Eutrophication in rural ponds has become a widespread environmental concern, particularly in regions affected by agricultural irrigation. This study proposes an innovative Submerged Macrophytes–Daphnia magna combined remediation technology, aiming to synergistically improve water quality in naturally eutrophic ponds. Experimental water was sourced [...] Read more.

Eutrophication in rural ponds has become a widespread environmental concern, particularly in regions affected by agricultural irrigation. This study proposes an innovative Submerged Macrophytes–Daphnia magna combined remediation technology, aiming to synergistically improve water quality in naturally eutrophic ponds. Experimental water was sourced from rural ponds with preserved natural phytoplankton and bacterial communities. Treatments included low- and high-density D. magna, two submerged macrophyte species (Myriophyllum aquaticum and Ceratophyllum demersum), and their combinations. Results showed that combined treatments had no significant effect on pH but improved water transparency by up to 63.6% and significantly increased dissolved oxygen. Nutrient removal was notably enhanced in combined groups, with low-density D. magna + M. aquaticum achieving TN, TP, and NO₃⁻-N reductions of 56.1%, 63.2%, and 58.7%, respectively. Both macrophytes effectively mitigated NH₄⁺-N accumulation caused by D. magna, with M. aquaticum showing stronger inhibition. Furthermore, low-density D. magna reduced phytoplankton density, cyanobacteria density, chlorophyll-a, and microcystins by 74.8%, 80.3%, 68.9%, and 71.2%, respectively. This combined bioremediation approach demonstrates high ecological efficiency, scalability potential, and practical applicability for rural pond restoration. Full article

(This article belongs to the Section Plant Ecology)

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14 pages, 1332 KB

Open AccessArticle

Understory Dwarf Bamboo Modulates Leaf Litter Decomposition via Interception-Induced Litter Redistribution and Space-Dependent Decomposition Dynamics: A Case Study from Jinfo Mountain, China

by Hai-Yan Song, Feng Qian, Chun-Yan Xia, Hong Xia, Jin-Chun Liu, Wei-Xue Luo and Jian-Ping Tao

Plants 2025, 14(20), 3135; https://doi.org/10.3390/plants14203135 (registering DOI) - 11 Oct 2025

Abstract

Understory vegetation, particularly dwarf bamboo, plays a crucial role in regulating forest nutrient cycles by intercepting litter and altering decomposition processes, yet its overall impacts remain understudied and insufficiently quantified. This study employs a combination of field surveys and decomposition bag experiments to [...] Read more.

Understory vegetation, particularly dwarf bamboo, plays a crucial role in regulating forest nutrient cycles by intercepting litter and altering decomposition processes, yet its overall impacts remain understudied and insufficiently quantified. This study employs a combination of field surveys and decomposition bag experiments to investigate how understory dwarf bamboo (Fargesia decurvata) alters the spatial–temporal patterns of leaf litter production and decomposition. We found that the dwarf bamboo intercepted more than 25% of canopy litterfall, altering its spatial distribution and reducing decomposition efficiency in the bamboo crown (BC). Leaf trait-decomposition relationships differed strongly across habitats, being positive for saturated fresh weight (SFW), leaf thickness (LFT), and leaf area (LA) and dry weight (DW) in bamboo habitats but weaker in the bamboo-free habitat (NB). Potassium release was significantly higher in the BC treatment, whereas carbon release showed the opposite trend. In contrast, nitrogen and phosphorus exhibited net enrichment across all treatments, with phosphorus enrichment being slower in BC than in bamboo-covered ground surface (BG) and NB. Our results demonstrate that the understory dwarf bamboo reshapes the spatial distribution of litter and nutrient release dynamics during decomposition, resulting in element-specific nutrient release patterns. These findings provide mechanistic insights into how understory dwarf bamboo mediates nutrient cycling dynamics in forest communities. Full article

(This article belongs to the Section Plant Ecology)

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18 pages, 7772 KB

Open AccessArticle

High Red–Blue Light Ratio Promotes Accelerated In Vitro Flowering and Seed-Set Development in Amaranthus hypochondriacus Under a Long-Day Photoperiod

by Alex R. Bermudez-Valle, Norma A. Martínez-Gallardo, Eliana Valencia-Lozano and John P. Délano-Frier

Plants 2025, 14(20), 3134; https://doi.org/10.3390/plants14203134 (registering DOI) - 11 Oct 2025

Abstract

Grain amaranths are recalcitrant to conventional in vitro plant regeneration by organogenesis de novo or through somatic embryogenesis. Consequently, floral organogenesis by these methods, representing the culminating developmental point in angiosperms, is rarely achieved. In the present study, the manipulation of in vitro [...] Read more.

Grain amaranths are recalcitrant to conventional in vitro plant regeneration by organogenesis de novo or through somatic embryogenesis. Consequently, floral organogenesis by these methods, representing the culminating developmental point in angiosperms, is rarely achieved. In the present study, the manipulation of in vitro flowering was explored as part of a strategy designed to overcome grain amaranth’s regeneration recalcitrance. It led to an efficient and reproducible in vitro protocol in which half-longitudinally dissected zygotic embryos generated fully developed Amaranthus hypochondriacus (Ah) plants. The use of high-irradiance illumination with LED lamps with a 3:1 red–blue irradiance ratio was a critical factor, leading to a 70% rate of early flowering events under flowering-inhibiting long-day photoperiod conditions. Contrariwise, no flowering was induced under LED white lights. All in vitro flowering Ah plants yielded viable seeds. To understand the basic molecular mechanisms of the phenomenon observed, gene expression patterns and principal component analysis of key flowering-related genes were analyzed after cultivation in vitro for 4, 8, and 12 weeks under both lighting regimes. These coded for photoreceptors, photomorphogenetic regulators, embryogenic modulators, and flowering activators/repressors. The results highlighted the upregulation of key flowering-regulatory genes, including CONSTANS, FLOWERING LOCUS T, and LEAFY, together with the downregulation of the floral repressor TERMINAL FLOWER1. Ribosome biogenesis- and seed-development-related genes were also differentially expressed, supporting a key role in this process for protein synthesis and embryogenesis. A model is proposed to explain how this light-regulated molecular framework enables in vitro flowering and seed production in Ah plants kept under long-day photoperiods. Full article

(This article belongs to the Special Issue Molecular Mechanisms Involved in Somatic Embryogenesis and Organogenesis of Plants)

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15 pages, 3775 KB

Open AccessArticle

Influence of Basal Medium and Organic Additives on In Vitro Germination and Plant Growth of Endangered Orchid Gastrochilus fuscopunctatus

by Jung Eun Hwang, Hyeong Bin Park, Jae-Hwa Tho, Myojin Kim, Hwan Joon Park, Seongjun Kim, Chang Woo Lee and Young-Joong Kim

Plants 2025, 14(20), 3133; https://doi.org/10.3390/plants14203133 (registering DOI) - 11 Oct 2025

Abstract

Gastrochilus fuscopunctatus is a rare epiphytic orchid in Korea threatened by habitat loss and illegal collection. This study aimed to establish an efficient in vitro propagation system by evaluating asymbiotic germination and seedling growth. Mature seeds germinated on both Hyponex (Hy) and Orchid [...] Read more.

Gastrochilus fuscopunctatus is a rare epiphytic orchid in Korea threatened by habitat loss and illegal collection. This study aimed to establish an efficient in vitro propagation system by evaluating asymbiotic germination and seedling growth. Mature seeds germinated on both Hyponex (Hy) and Orchid Seed Sowing Medium (OSM), but protocorm development was more active on Hy, regardless of 1 µM NAA supplementation. For seedling culture, Murashige and Skoog (MS), Hy, and Orchid Maintenance Medium (OM) were tested with apple homogenate (AH), banana homogenate (BH), and coconut water (CW). At 7 months, Hy supported the greatest biomass and root formation, while Hy + BH produced the highest values (0.066 g fresh weight, 1.3 cm root length). Although BH improved growth on MS and OM, the effects were less pronounced. At 14 months, Hy + BH again yielded superior results (1.93 g fresh weight, 5.3 cm root length, 17.2 leaves), clearly outperforming all other combinations. AH and CW showed inconsistent or limited effects across media, indicating strong medium–additive interactions. These findings demonstrate that Hy + BH is the most effective combination for both early and prolonged growth of G. fuscopunctatus, providing a practical framework for ex situ conservation and reintroduction. Full article

(This article belongs to the Section Plant Development and Morphogenesis)

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17 pages, 1397 KB

Open AccessArticle

Activity-Based Profiling of Papain-like Cysteine Proteases During Late-Stage Leaf Senescence in Barley

by Igor A. Schepetkin and Andreas M. Fischer

Plants 2025, 14(20), 3132; https://doi.org/10.3390/plants14203132 (registering DOI) - 11 Oct 2025

Abstract

Leaf senescence is a developmental process that allows nutrients to be remobilized and transported to sink organs. Previously, papain-like cysteine proteases (PLCPs) have been found to be highly expressed during leaf senescence in different plant species. In this study, we analyzed active PLCPs [...] Read more.

Leaf senescence is a developmental process that allows nutrients to be remobilized and transported to sink organs. Previously, papain-like cysteine proteases (PLCPs) have been found to be highly expressed during leaf senescence in different plant species. In this study, we analyzed active PLCPs in barley (Hordeum vulgare L.) leaves during the terminal stage of natural senescence. Anion exchange chromatography of protein extracts from barley leaves, harvested six weeks after anthesis, followed by activity assays using the substrates Z-FR-AMC and Z-RR-AMC, revealed a single prominent peak corresponding to active PLCPs. This hydrolytic activity was completely inhibited by E-64, a potent and irreversible inhibitor of cysteine proteases. Fractions enriched for PLCP activity were affinity-labeled with DCG-04 and subjected to SDS-PAGE fractionation, separating two major bands at 43 and 38 kDa. These bands were analyzed using tandem mass spectrometry, allowing the identification of eleven PLCPs. Identified enzymes belong to eight PLCP subfamilies, including CTB/cathepsin B-like (HvPap-19 and -20), RD19/cathepsin F-like (HvPap-1), ALP/cathepsin H-like (HvPap-12 or aleurain), SAG12/cathepsin L-like A (HvPap-17), CEP/cathepsin L-like B (HvPap-14), RD21/cathepsin L-like D (HvPap-6 and -7), cathepsin L-like E (HvPap-13 and -16), and XBCP3 (HvPap-8). Among the identified PLCPs, HvPap-6 was the most abundant. Peptides corresponding to HvPap-6 were identified in both the 43 kDa and 38 kDa bands in approximately the same quantity based on total spectral count. Thus, our results indicate that two active HvPap-6 isoforms can be isolated from barley leaves at late senescence. Full article

(This article belongs to the Section Plant Development and Morphogenesis)

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17 pages, 1780 KB

Open AccessArticle

Effects of Different Slope Aspects on Leaf Non-Structural Carbohydrate Characteristics and Leaf–Soil Stoichiometry of Sapindus mukorossi

by Heng Wang, Chengyao Liu, Dingming Wei, Yunbin Zhou, Tingwen He, Tangjie Zhao, Chengbo Peng, Lianchun Wang and Yuan Zheng

Plants 2025, 14(20), 3131; https://doi.org/10.3390/plants14203131 (registering DOI) - 11 Oct 2025

Abstract

Slope aspect and slope position have an important influence on plant growth by changing the microclimate and soil conditions such as light, temperature, moisture, and nutrients. In this study, 15-year-old Sapindus mukorossi forests with different slope aspects and positions were selected and the [...] Read more.

Slope aspect and slope position have an important influence on plant growth by changing the microclimate and soil conditions such as light, temperature, moisture, and nutrients. In this study, 15-year-old Sapindus mukorossi forests with different slope aspects and positions were selected and the differences in tree height and diameter at breast height (DBH), leaf non-structural carbohydrate (NSC) characteristics, and leaf–soil nitrogen (N), phosphorus (P), and potassium (K) stoichiometric characteristics between sunny and shady slopes, and upper, middle, and down slope positions were compared and analyzed. The results show that the tree height and DBH of S. mukorossi were better in the same slope aspect and lower slope position, while in the same slope position, the tree height and diameter at DBH were better on the shady slopes. In the upper slope position, the starch content on the shady slope was significantly higher than that on the sunny slope, and the NSC content was significantly higher than that on the sunny slope. On shady and sunny slopes, S. mukorossi is mainly limited by N. The leaf and soil P content of S. mukorossi on the sunny slope was the highest and significantly higher than that on the upper slope. The coefficient of variation of each index of S. mukorossi on the shady slope and the sunny slope was medium and below. Soil N/P, soil N, soil N/K, soluble sugar/starch, leaf P, leaf K, leaf N, and soil K had strong plasticity under different slope aspects. Therefore, it indicated that the shady slope and down slope were more suitable for S. mukorossi. Full article

(This article belongs to the Section Plant Physiology and Metabolism)

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22 pages, 4480 KB

Open AccessArticle

Genome-Wide Analysis Reveals Chitinases as Putative Defense-Related Proteins Against Fungi in the Genomes of Coffea arabica and Its Progenitors

by Fernanda Rodrigues Silva, Mario Lucio V. de Resende, Katia V. Xavier, Jeremy T. Brawner and Mariana de Lima Santos

Plants 2025, 14(20), 3130; https://doi.org/10.3390/plants14203130 (registering DOI) - 10 Oct 2025

Abstract

Chitinases have been demonstrated to enhance plant resistance to fungi in various pathosystems. Although there is evidence of the effectiveness of these proteins in coffee–fungus interactions, no genome-wide identification or characterization of coffee chitinases has been performed. In this study, we employed phylogenetic [...] Read more.

Chitinases have been demonstrated to enhance plant resistance to fungi in various pathosystems. Although there is evidence of the effectiveness of these proteins in coffee–fungus interactions, no genome-wide identification or characterization of coffee chitinases has been performed. In this study, we employed phylogenetic analysis, domain architecture, gene structure analysis, and subcellular localization to identify and characterize putative genes and proteins in the genomes of Coffea arabica and its progenitors, Coffea canephora and Coffea eugenioides. A total of 113, 47, and 69 putative chitinase proteins were identified in C. arabica, C. canephora, and C. eugenioides, respectively. These chitinases were classified according to their catalytic domains, GH18 and GH19, and into Classes I, II, III, IV, and V, as determined through phylogenetic analysis based on the Arabidopsis thaliana classification. Furthermore, based on orthologous analysis, we identified ten, six, and seven putative chitinases associated with fungal defense responses in C. arabica, C. canephora, and C. eugenioides, respectively. These findings are valuable for future studies focusing on coffee chitinases, particularly on genetic programs involved in plant pathogen resistance. Full article

(This article belongs to the Special Issue Bioinformatics and Functional Genomics in Modern Plant Science)

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24 pages, 4622 KB

Open AccessArticle

Elucidating the Mechanistic Role of Exogenous Melatonin in Salt Stress Tolerance of Maize (Zea mays L.) Seedlings: An Integrated Physiological, Metabolomic, and Proteomic Profiling Analysis

by Zhichao Wang, Linhao Zong, Qiqi Cai, Yinjie Fu, Zhiping Gao and Guoxiang Chen

Plants 2025, 14(20), 3129; https://doi.org/10.3390/plants14203129 (registering DOI) - 10 Oct 2025

Abstract

Maize (Zea mays L.), as a globally significant cereal crop, exhibits high sensitivity to salt stress during early seedling stages. Although melatonin (MT) has demonstrated potential in mitigating abiotic stresses, the specific mechanisms underlying MT-mediated alleviation of salt stress in maize seedlings [...] Read more.

Maize (Zea mays L.), as a globally significant cereal crop, exhibits high sensitivity to salt stress during early seedling stages. Although melatonin (MT) has demonstrated potential in mitigating abiotic stresses, the specific mechanisms underlying MT-mediated alleviation of salt stress in maize seedlings remain unclear. In this study, we established four treatment groups: control (CK), melatonin treatment (MT), salt stress (NaCl), and combined treatment (NaCl_MT). Metabolomic and proteomic analyses were performed, supplemented by photosynthesis-related experiments as well as antioxidant-related experiments. Metabolomic analysis identified key metabolites in MT-mediated salt stress mitigation. Both metabolomic and proteomic analyses underscored the critical roles of photosynthetic and antioxidant pathways. Salt stress significantly decreased the net photosynthetic rate (Pn) by 67.7%, disrupted chloroplast ultrastructure, and reduced chlorophyll content by 41.6%. Conversely, MT treatment notably mitigated these detrimental effects. Moreover, MT enhanced the activities of antioxidant enzymes by approximately 10–20% and reduced the accumulation of oxidative stress markers by around 10–25% in maize seedlings under salt stress. In conclusion, this study conducted a systematic and multidimensional investigation into the mitigation of salt stress in maize seedlings by MT. Our results revealed that MT enhances antioxidant systems, increases chlorophyll content, and alleviates damage to chloroplast ultrastructure, thereby improving photosystem II performance and strengthening photosynthesis. This ultimately manifests as improved seedling phenotypes under salt stress. These findings provide a meaningful entry point for breeding salt-tolerant maize varieties and mitigating the adverse effects of salinized soil on maize growth and yield. Full article

(This article belongs to the Special Issue Abiotic Stress Responses in Plants—Second Edition)

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19 pages, 1450 KB

Open AccessArticle

Bryophytes of the Loess Cliffs in the Pannonian Area of Austria

by Harald G. Zechmeister and Michaela Kropik

Plants 2025, 14(20), 3128; https://doi.org/10.3390/plants14203128 - 10 Oct 2025

Abstract

Austrian loess cliffs represent unique habitats supporting a rich bryophyte flora, including numerous rare and endangered species. We conducted a comprehensive survey of 86 loess cliff sites in the Pannonian area of Lower Austria, Burgenland, and Vienna, recording 79 bryophyte species. The results [...] Read more.

Austrian loess cliffs represent unique habitats supporting a rich bryophyte flora, including numerous rare and endangered species. We conducted a comprehensive survey of 86 loess cliff sites in the Pannonian area of Lower Austria, Burgenland, and Vienna, recording 79 bryophyte species. The results highlight that Austrian loess cliffs, despite their small spatial extent, are key refugia for light-demanding, desiccation-tolerant bryophytes. Hilpertia velenovskyi, a critically endangered species, was recorded at six new sites, expanding its known Austrian distribution. Our study also documents the first Austrian occurrences of several Pterygoneurum species. Seven bryophyte communities were distinguished: Aloinetum rigidae, Hilpertio velenovskyi–Pterygoneuretum compacti, and the newly described subassociations Didymodontetum glauci didymodontetosum cordati and Eurhynchietum schleicheri didymodontetosum cordati, as well as Pterygoneuro–Acaulonetum triquetri ass. nov. Multivariate analyses suggest carbonate content as the most consistent environmental driver. Despite their ecological significance, loess cliffs are increasingly threatened by habitat loss, overgrowth by vascular plants, and shading from invasive trees. Our study provides a detailed syntaxonomic and ecological framework for bryophyte communities on loess cliffs, underlining their role as refugia for rare species and the urgent need to protect remaining sites in the Pannonian region. Full article

(This article belongs to the Special Issue Bryophyte Biology, 2nd Edition)

15 pages, 2069 KB

Open AccessArticle

A Cryopreservation and Regeneration Protocol for Embryogenic Callus of Larix olgensis

by Chen Wang, Wenna Zhao, Yu Liu, Hao Dong, Yajing Ning, Chengpeng Cui, Hanguo Zhang, Meng Li and Shujuan Li

Plants 2025, 14(20), 3127; https://doi.org/10.3390/plants14203127 - 10 Oct 2025

Abstract

Larix olgensis is a valuable timber species in northern China, typically propagated through somatic embryogenesis (SE). However, long-term subculture can lead to a loss of embryogenic potential. This study aimed to establish a simple and stable protocol for the cryopreservation and regeneration of [...] Read more.

Larix olgensis is a valuable timber species in northern China, typically propagated through somatic embryogenesis (SE). However, long-term subculture can lead to a loss of embryogenic potential. This study aimed to establish a simple and stable protocol for the cryopreservation and regeneration of L. olgensis embryogenic callus (EC) that preserves its SE potential and regenerative capacity. The slow-freezing method was employed for cryopreservation. A cryopreservation protocol for L. olgensis EC was developed by optimizing the preculture duration and conditions, cryoprotectant composition and thawing temperature. The results showed that optimal outcomes were achieved using a 24 h stepwise preculture on medium containing 0.2 and 0.4 mol∙L⁻¹ sucrose, followed by cryoprotectant treatment with 0.4 mol∙L⁻¹ sucrose, 2.5% (v/v) dimethyl sulfoxide (DMSO) and 10% polyethylene glycol 6000 (PEG₆₀₀₀), and thawing at 37 °C. EC cryopreserved using this protocol achieved a 100% recovery rate. Moreover, the cryopreserved recoverable EC successfully underwent SE, progressing through germination and rooting. Cryopreservation duration (storage duration in liquid nitrogen) did not affect cell viability and proliferation rate, confirming the protocol’s suitability for long-term cryopreservation of L. olgensis EC. This study provides a valuable reference for the cryopreservation and regeneration of L. olgensis EC, with potential applications for other coniferous species. It establishes a robust foundation for the large-scale propagation of conifers. Full article

(This article belongs to the Special Issue Sexual and Asexual Reproduction in Forest Plants—2nd Edition)

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30 pages, 9482 KB

Open AccessArticle

First Phenotypic Characterization of the Edible Fruits of Lardizabala biternata: A Baseline for Conservation and Domestication of a Neglected and Endemic Vine

by Jaime Herrera and Leonardo D. Fernández

Plants 2025, 14(20), 3126; https://doi.org/10.3390/plants14203126 (registering DOI) - 10 Oct 2025

Abstract

Lardizabala biternata is a culturally valued, endemic vine of the Chilean Winter Rainfall–Valdivian Forest biodiversity hotspot, traditionally harvested for its sweet, edible fruits. Despite its ecological singularity as the sole species in a monotypic genus, the species remains biologically and agronomically understudied, with [...] Read more.

Lardizabala biternata is a culturally valued, endemic vine of the Chilean Winter Rainfall–Valdivian Forest biodiversity hotspot, traditionally harvested for its sweet, edible fruits. Despite its ecological singularity as the sole species in a monotypic genus, the species remains biologically and agronomically understudied, with no formal cultivation systems. There is currently no baseline information on its fruit morphology, which limits the design of conservation strategies and the development of its agronomic potential. This study provides the first phenotypic characterisation of L. biternata fruits, aimed at supporting germplasm evaluation, ex situ conservation, and sustainable domestication of this rare species. A total of 205 fruits were sampled across two seasons and two geographically distant populations. We measured 14 traits, including weight, length, diameter, pulp content, and seed metrics, and analysed morphological variation using t-tests, ANOVA, regression, and principal component analysis or PCA. Fruits averaged 21.0 g in weight, 54.2 mm in length, and 23.8 mm in diameter. Edible pulp constituted 44.4% of total fruit weight and showed strong positive correlations with fruit size, seed number, and seed weight. Significant differences were observed across seasons and populations, with cooler, wetter conditions associated with larger fruits and higher pulp yield. Our findings reveal substantial morphological variability and climate sensitivity, providing a crucial baseline for selecting desirable traits. This work informs ongoing efforts in plant domestication, sustainable agriculture, and the conservation of underutilised species of cultural and ecological importance. Full article

(This article belongs to the Section Horticultural Science and Ornamental Plants)

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17 pages, 4611 KB

Open AccessArticle

Optimizing Rhizome Quality in Ligusticum chuanxiong Hort. Through High Maltose Concentration

by Hui-Yeong Jeong, Ho-Jun Son, Jun-Won Kang and Ji-Ah Kim

Plants 2025, 14(20), 3125; https://doi.org/10.3390/plants14203125 - 10 Oct 2025

Abstract

Ligusticum chuanxiong Hort. (L. chuanxiong) is a traditional medicinal food in East Asia. This study provides a comprehensive analysis of the effects of various carbohydrates on L. chuanxiong. It covers rhizome induction, acclimatization, and field growth. In the context of [...] Read more.

Ligusticum chuanxiong Hort. (L. chuanxiong) is a traditional medicinal food in East Asia. This study provides a comprehensive analysis of the effects of various carbohydrates on L. chuanxiong. It covers rhizome induction, acclimatization, and field growth. In the context of this study, the most effective carbohydrates for promoting rhizome induction in vitro to field growth ex vitro of L. chuanxiong were identified as maltose treatments with a concentration of 4% and 6%. It has been demonstrated that, particularly at a concentration of 4%, this treatment is particularly beneficial for in vitro rhizome development of L. chuanxiong. Following acclimatization, the 6% maltose treatments exhibited the highest fresh weight (3.3 ± 0.2 g). Following the process of field growth, there was a significant increase in the fresh weight of the rhizome under the 6% maltose treatment (160.8 ± 22.2 g) in comparison with the other treatments. This investigation is the first study on rhizome production of L. chuanxiong. It is clear that the appropriate carbohydrate treatment protocol is key to optimizing rhizome production and providing fundamental data for the best propagation of L. chuanxiong. Full article

(This article belongs to the Collection Plant Tissue Culture)

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17 pages, 2106 KB

Open AccessArticle

Differential Phosphorus Acquisition Strategies and Adaptive Mechanisms Evolved by Three Lespedeza Species to Tackle Phosphorus Deficiency

by Jingchong Li, Hao Shi, Guanqiang Zuo, Shasha Li, Yafei Chen and Shiwen Wang

Plants 2025, 14(20), 3124; https://doi.org/10.3390/plants14203124 - 10 Oct 2025

Abstract

Phosphorus (P) is essential for plant growth but is frequently limited in soils. Lespedeza species are well-known for their ecological and economic benefits, as well as their tolerance to nutrient-poor soils. This study investigated the P acquisition strategies and adaptive mechanisms of three [...] Read more.

Phosphorus (P) is essential for plant growth but is frequently limited in soils. Lespedeza species are well-known for their ecological and economic benefits, as well as their tolerance to nutrient-poor soils. This study investigated the P acquisition strategies and adaptive mechanisms of three Lespedeza species (L. davurica, L. bicolor, and L. cuneata), focusing on biomass allocation, P distribution, root exudation, and absorption kinetics under P deficiency. Under P deficiency, L. davurica and L. bicolor allocated more biomass to roots to enhance P acquisition, whereas L. cuneata increased specific root length and area. Moreover, all three species preferentially allocated P to roots, but L. bicolor showed higher P content in stems and leaves than the others. P absorption kinetics indicated that Michaelis constant (K_m) and equilibrium concentration (C_min) were significantly decreased in all three species under P deficiency, with L. bicolor exhibiting the strongest P affinity and acquisition capacity. Secretion analysis revealed that while L. davurica and L. cuneata secreted higher levels of organic acids under P deficiency, exudates from L. bicolor were significantly enriched in acid phosphatase activity. Overall, the three Lespedeza species developed distinct P acquisition and adaptive strategies to cope with P deficiency, with L. bicolor demonstrating the greatest low-P tolerance and most efficient adaptive mechanisms. Full article

(This article belongs to the Special Issue Nitrogen and Phosphorus Transport and Signaling in Plants)

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26 pages, 13654 KB

Open AccessArticle

Initiation of Direct Shoot Organogenesis in Coconut Using Immature Inflorescence

by Eveline Y. Y. Kong, Julianne Maree Biddle, Sisunandar Sisunandar, Sundaravelpandian Kalaipandian, Amirhossein Bazrafshan, Zhihua Mu and Steve W. Adkins

Plants 2025, 14(20), 3123; https://doi.org/10.3390/plants14203123 - 10 Oct 2025

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Abstract

Coconut (Cocos nucifera L.) is one of the most widely cultivated crops, with increasing popularity and demand for its products, which necessitates increased production. However, the lack of high-quality planting materials is a major limitation in replanting the senile palms worldwide. This [...] Read more.

Coconut (Cocos nucifera L.) is one of the most widely cultivated crops, with increasing popularity and demand for its products, which necessitates increased production. However, the lack of high-quality planting materials is a major limitation in replanting the senile palms worldwide. This study examined the possibility of using a direct shoot organogenesis pathway as an alternative to somatic embryogenesis to produce clonal coconut plantlets using immature inflorescence explants obtained from Indonesia and Australia, through investigation of the explant types, exogenous plant growth regulators, and additives. Histological analysis showed suitable stages of immature inflorescence explants to be used, which led to the formation of shoot-like structures resembling true vegetative shoots, in all treatments consisting of exogenous plant growth regulators except for those without activated charcoal. The culture medium supplemented with thidiazuron (100 μM) alone or those supplemented with various combinations of other plant growth regulators showed similar shoot induction percentages (ca. 63 to 80%) or shoot-like structures per explant (ca. 6 to 8). The addition of adenine sulphate (217 μM) was found to significantly improve shoot induction (ca. 50%) from immature inflorescence explants compared to the control (ca. 5%), whereas phloroglucinol was found to negatively impact shoot induction, and L-glutamine showed a positive influence. The current study showed several improvements, which warrant further studies to develop commercial protocol for mass production of clonal coconut plantlets through direct organogenesis. Full article

(This article belongs to the Special Issue Plant Tissue Culture V)

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16 pages, 6997 KB

Open AccessArticle

Ascorbic Acid Priming Boosts Cotton Seed Chilling Tolerance via Membrane Stability and Antioxidant Cycles

by Peng Han, Haixia Ma, Lu Lu, Jincheng Zhu, Xinhui Nie, Jianwei Xu and Zhibo Li

Plants 2025, 14(20), 3122; https://doi.org/10.3390/plants14203122 - 10 Oct 2025

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Abstract

Low-temperature stress severely restricts cotton seed germination and seedling establishment, especially in early spring. Ascorbic acid (AsA) priming is a promising strategy to enhance stress tolerance, yet its mechanisms in cotton remain unclear. This study examined the effects of AsA priming on seed [...] Read more.

Low-temperature stress severely restricts cotton seed germination and seedling establishment, especially in early spring. Ascorbic acid (AsA) priming is a promising strategy to enhance stress tolerance, yet its mechanisms in cotton remain unclear. This study examined the effects of AsA priming on seed germination at 15 °C. Seeds were treated with 0, 25, 50, or 100 mg/L AsA for 3, 6, 12, or 24 h. Results showed that 50 mg/L AsA for 24 h significantly improved germination potential, rate, index, and promptness index (p < 0.05). Compared with water-primed seeds, AsA-primed seeds exhibited greater radicle length (+17.67%) and fresh weight (+136.26%) under chilling stress. This treatment markedly increased antioxidant enzyme activities, including POD (+196.74%), SOD (+43.81%), and CAT (+49.43%), while also promoting the accumulation of Ascorbate–Glutathione cycle-related enzymes and metabolites, thereby reinforcing the antioxidant defense system. Multidimensional statistical analyses further indicated that AsA enhanced root growth by stimulating antioxidant defenses while inducing a trade-off that slightly reduced fresh weight, suggesting a balance between growth and oxidative protection. Overall, AsA priming improves cotton seed cold tolerance by activating enzymatic and non-enzymatic antioxidant systems and mediating a growth–defense trade-off, underscoring its potential as an effective priming agent for early sowing under low-temperature stress. Full article

(This article belongs to the Special Issue Plant Functioning Under Abiotic Stress)

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14 pages, 1800 KB

Open AccessArticle

Chilean Aloysia Essential Oils: A Medicinal Plant Resource for Postharvest Disease Control

by Valentina Silva, Catalina Ferreira, Susana Flores, Evelyn Muñoz, Constanza Reyes, Carmen Trujillo, Esperanza Gálvez, Katy Díaz and Alejandro Madrid

Plants 2025, 14(20), 3121; https://doi.org/10.3390/plants14203121 - 10 Oct 2025

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Abstract

Postharvest fungal rot causes significant economic losses in the agroindustry. Current control methods involving the use of synthetic fungicides are becoming increasingly ineffective and pose environmental risks. This necessitates exploring sustainable alternatives, such as essential oils derived from medicinal plants, to achieve safer [...] Read more.

Postharvest fungal rot causes significant economic losses in the agroindustry. Current control methods involving the use of synthetic fungicides are becoming increasingly ineffective and pose environmental risks. This necessitates exploring sustainable alternatives, such as essential oils derived from medicinal plants, to achieve safer and effective disease control. This research examined the chemical composition and efficacy of essential oils from Aloysia citriodora, Aloysia polystachya and their compounds against the postharvest rot fungi Monilinia fructicola, Monilinia laxa, and Botrytis cinerea. The main compounds of essential oils were analyzed by GC/MS and revealed differences in their composition. A. citriodora is characterized by the presence of spathulenol and caryophyllene oxide. In contrast, A. polystachya is characterized by the predominance of carvone. The results show that the essential oil of A. citriodora and the compound farnesol are able to inhibit the three pathogens. Notably, against M. fructicola, the EC₅₀ values were 61.89 μg/mL and 72.18 μg/mL, respectively. Against B. cinerea, the EC₅₀ values were 85.34 μg/mL and 47.6 μg/mL. Molecular docking also showed that farnesol has affinity for the enzyme succinate dehydrogenase suggesting a possible mechanism of action. This compound and A. citriodora essential oil show potential in the control of phytopathogens. Full article

(This article belongs to the Section Phytochemistry)

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19 pages, 3257 KB

Open AccessArticle

Integrated Multi-Omics Analysis Reveals the Survival Strategy of Dongxiang Wild Rice (DXWR, Oryza rufipogon Griff.) Under Low-Temperature and Anaerobic Stress

by Jilin Wang, Cheng Huang, Hongping Chen, Lijuan Tang and Dianwen Wang

Plants 2025, 14(20), 3120; https://doi.org/10.3390/plants14203120 - 10 Oct 2025

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Abstract

Dongxiang wild rice (DXWR, Oryza rufipogon Griff.), the northernmost known wild rice species, exhibits exceptional tolerance to combined low-temperature and anaerobic stress during seed germination, providing a unique model for understanding plant adaptation to complex environmental constraints. Here, we employed an integrated multi-omics [...] Read more.

Dongxiang wild rice (DXWR, Oryza rufipogon Griff.), the northernmost known wild rice species, exhibits exceptional tolerance to combined low-temperature and anaerobic stress during seed germination, providing a unique model for understanding plant adaptation to complex environmental constraints. Here, we employed an integrated multi-omics approach combining genomic, transcriptomic, and metabolomic analyses to unravel the synergistic regulatory mechanisms underlying this tolerance. Genomic comparative analysis categorized DXWR genes into three evolutionary groups: 18,480 core genes, 15,880 accessory genes, and 6822 unique genes. Transcriptomic profiling identified 10,593 differentially expressed genes (DEGs) relative to the control, with combined stress triggering the most profound changes, specifically inducing the upregulation of 5573 genes and downregulation of 5809 genes. Functional characterization revealed that core genes, including DREB transcription factors, coordinate energy metabolism and antioxidant pathways; accessory genes, such as glycoside hydrolase GH18 family members, optimize energy supply via adaptive evolution; and unique genes, including specific UDP-glycosyltransferases (UDPGTs), confer specialized stress resilience. Widely targeted metabolomics identified 889 differentially accumulated metabolites (DAMs), highlighting significant accumulations of oligosaccharides (e.g., raffinose) to support glycolytic energy production and a marked increase in flavonoids (153 compounds identified, e.g., procyanidins) enhancing antioxidant defense. Hormonal signals, including jasmonic acid and auxin, were reconfigured to balance growth and defense responses. We propose a multi-level regulatory network based on a “core-unique-adaptive” genetic framework, centered on ERF family transcriptional hubs and coordinated through a metabolic adaptation strategy of “energy optimization, redox homeostasis, and growth inhibition relief”. These findings offer innovative strategies for improving rice stress tolerance, particularly for enhancing germination of direct-seeded rice under early spring low-temperature and anaerobic conditions, by utilizing key genes such as GH18s and UDPGTs, thereby providing crucial theoretical and technological support for addressing food security challenges under climate change. Full article

(This article belongs to the Special Issue Genetic and Genomic Strategies for Enhancing Seed Quality and Grain Nutrition in Cereal Crops)

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15 pages, 2497 KB

Open AccessArticle

Colored Shade Nets and LED Lights at Different Wavelengths Increase the Production and Quality of Canada Goldenrod (Solidago canadensis L.) Flower Stems

by Fabíola Villa, Luciana Sabini da Silva Murara, Giordana Menegazzo da Silva, Edvan Costa da Silva, Larissa Hiromi Kiahara Sackser, Laís Romero Paula, Mateus Lopes Borduqui Cavalcante and Daniel Fernandes da Silva

Plants 2025, 14(20), 3119; https://doi.org/10.3390/plants14203119 - 10 Oct 2025

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Abstract

Canada goldenrod (Solidago canadensis L.), a short-day plant commonly cultivated as a cut flower, depends on proper lighting management to obtain long stems and higher commercial value. Thus, this study aimed to determine the effect of modifying the light spectrum through the [...] Read more.

Canada goldenrod (Solidago canadensis L.), a short-day plant commonly cultivated as a cut flower, depends on proper lighting management to obtain long stems and higher commercial value. Thus, this study aimed to determine the effect of modifying the light spectrum through the installation of light-emitting diodes (LEDs) and the use of colored shade nets on the production and quality of Canada goldenrod stems. The treatments used were colored shade nets and different LED lighting treatments. Production per plant and productivity per square meter were determined. Twenty stems were selected and evaluated for: stem length; inflorescence length and width; number of floral ramets per inflorescence; number of leaves; stem base diameter (mm); and fresh stem biomass (g). Canada goldenrod plants require an extension of the light period with artificial lighting to produce higher-quality stems, regardless of whether the bulbs emit red or white light. The use of nets with 50% red and white shading promoted higher production and elongation of Canada goldenrod stems, with a production that reached up to 4.2 floral stems per plant and 100.3 floral stems per square meter using the red shade net and white LED. These floral stems were of high commercial standard, with a length of up to 81.35 cm with the red shade net and red LED, and were 31 cm in diameter for the inflorescences, approximately, under black or white shade nets and white or red LEDs. More robust floral stems with greater biomass were observed using any shade net color and LED lamps. Full article

(This article belongs to the Special Issue Physiology and Seedling Production of Plants)

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26 pages, 1510 KB

Open AccessReview

Nanoparticles and Nanocarriers for Managing Plant Viral Diseases

by Ubilfrido Vasquez-Gutierrez, Gustavo Alberto Frias-Treviño, Luis Alberto Aguirre-Uribe, Sonia Noemí Ramírez-Barrón, Jesús Mendez-Lozano, Agustín Hernández-Juárez and Hernán García-Ruíz

Plants 2025, 14(20), 3118; https://doi.org/10.3390/plants14203118 - 10 Oct 2025

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Abstract

The nourishment of the human population depends on a handful of staple crops, such as maize, rice, wheat, soybeans, potatoes, tomatoes, and cassava. However, all crop plants are affected by at least one virus causing diseases that reduce yield, and in some parts [...] Read more.

The nourishment of the human population depends on a handful of staple crops, such as maize, rice, wheat, soybeans, potatoes, tomatoes, and cassava. However, all crop plants are affected by at least one virus causing diseases that reduce yield, and in some parts of the world, this leads to food insecurity. Conventional management practices need to be improved to incorporate recent scientific and technological developments such as antiviral gene silencing, the use of double-stranded RNA (dsRNA) to activate an antiviral response, and nanobiotechnology. dsRNA with antiviral activity disrupt viral replication, limit infection, and its use represents a promising option for virus management. However, currently, the biggest limitation for viral diseases management is that dsRNA is unstable in the environment. This review is focused on the potential of nanoparticles and nanocarriers to deliver dsRNA, enhance stability, and activate antiviral gene silencing. Effective carriers include metal-based nanoparticles, including silver, zinc oxide, and copper oxide. The stability of dsRNA and the efficiency of gene-silencing activation are enhanced by nanocarriers, including layered double hydroxides, chitosan, and carbon nanotubes, which protect and transport dsRNA to plant cells. The integration of nanocarriers and gene silencing represents a sustainable, precise, and scalable option for the management of viral diseases in crops. It is essential to continue interdisciplinary research to optimize delivery systems and ensure biosafety in large-scale agricultural applications. Full article

(This article belongs to the Special Issue Novel Insight into Elicitors of Plant Immunity and the Activated Defensive Pathways)

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24 pages, 3171 KB

Open AccessArticle

Investigating the Biocontrol and Plant Growth-Promoting Potential of Pseudomonas yamanorum for Sustainable Management of Tomato Early Blight (Alternaria alternata)

by Lobna Hajji-Hedfi, Takwa Wannassi, Amira Khlif, Nyasha J. Kavhiza and Nazih Y. Rebouh

Plants 2025, 14(20), 3117; https://doi.org/10.3390/plants14203117 - 10 Oct 2025

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Abstract

Tomato (Solanum lycopersicum L.) is among the most economically significant and nutritionally valuable vegetable crops grown globally. However, fungal diseases such as Early Blight caused by Alternaria alternata are a major factor limiting yield and fruit quality in tomato production. This study [...] Read more.

Tomato (Solanum lycopersicum L.) is among the most economically significant and nutritionally valuable vegetable crops grown globally. However, fungal diseases such as Early Blight caused by Alternaria alternata are a major factor limiting yield and fruit quality in tomato production. This study investigates the biocontrol potential of locally isolated rhizobacterium Pseudomonas yamanorum against A. alternata, the causal agent of early blight in tomato, under both in vitro and in planta conditions. In vitro assays demonstrated significant antifungal activity; in the dual confrontation assay, P. yamanorum (10⁸ CFU/mL) reduced A. alternata mycelial growth by 68.7%, while spore germination was inhibited by 88.7%. In planta trials demonstrated that plants treated with P. yamanorum (10⁷ CFU/mL) alone exhibited the lowest disease severity (2.5). The treatments also significantly enhanced plant growth, with shoot length reaching 45 cm versus 26 cm in infected controls. Biochemical analyses revealed increased catalase (94.84 units mg⁻¹ protein min⁻¹), peroxidase (5.83), and ascorbate peroxidase (67.01) activities in treated plants. Total polyphenol and protein contents also increased (0.81 mg/g and 15.82 mg/g, respectively). Furthermore, P. yamanorum treatments maintained fruit quality parameters such as firmness (3.13), sugar content (6.43 °Brix), and juice yield (55.88%), while reducing malondialdehyde (2.02 µmol/g Dry Weight) and electrical conductivity (0.59 mS/cm). These findings highlight P. yamanorum as a promising biocontrol agent and plant growth-promoting bacteria that improve disease resistance, which can be combined with salicylic acid to further enhance crop vigor and fruit quality under biotic stress. Full article

(This article belongs to the Special Issue Biological Control of Plant Diseases Caused by Pathogenic Microorganisms—2nd Edition)

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Open AccessArticle

Population-Specific Salinity Tolerance in the Extremophile Colobanthus quitensis: Evidence of Adaptive Plasticity

by Marely Cuba-Díaz, Yadiana Ontivero, Eduardo Fuentes-Lillo, Macarena Klagges, Paulina Arriagada, Gustavo Cabrera-Barja and Benjamín Sepúlveda

Plants 2025, 14(20), 3116; https://doi.org/10.3390/plants14203116 - 10 Oct 2025

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Abstract

Salinity is a major abiotic stress that limits plant growth and survival. Colobanthus quitensis, the only native dicotyledon in the Antarctic Peninsula and southern South America, naturally inhabits environments with contrasting salinity regimes. This study compared the salt stress responses of three [...] Read more.

Salinity is a major abiotic stress that limits plant growth and survival. Colobanthus quitensis, the only native dicotyledon in the Antarctic Peninsula and southern South America, naturally inhabits environments with contrasting salinity regimes. This study compared the salt stress responses of three geographically distinct populations—Antarctic (pA), Magellanic coastal (pPA), and Andean inland (pC)—exposed to 0, 50, and 150 mM NaCl under controlled conditions. Morpho-physiological traits, photosynthetic parameters, osmolyte accumulation, oxidative damage markers, and antioxidant responses were evaluated. Population-specific strategies were observed. In pA, salinity reduced shoot biomass by 58% and doubled lipid peroxidation levels at 50 mM, indicating high oxidative stress. In pPA, shoot growth was maintained even at 150 mM, although chlorophyll and carotenoid contents decreased by approximately 20%, along with a reduction in total antioxidant capacity. In contrast, pC showed a coordinated tolerance response, maintaining biomass while accumulating the highest proline levels (742 µmol g⁻¹ FW at 150 mM) and enhancing total antioxidant capacity by 35% compared to the control. Multivariate analyses supported the contrasting strategies among populations. These results provide novel evidence of local adaptation and ecological plasticity in C. quitensis, particularly highlighting the hidden resilience of non-coastal populations. The findings support the potential of this extremophile species as a model system for investigating salinity tolerance and as a promising genetic resource for developing biotechnological strategies aimed at improving crop resilience under saline conditions. Full article

(This article belongs to the Special Issue Mitigation Strategies and Tolerance of Plants to Abiotic Stresses—2nd Edition)

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Plants, Volume 14, Issue 20 (October-2 2025) – 30 articles

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