AUXIN RESPONSE FACTOR 1 Acts as a Positive Regulator in the Response of Poplar to Trichoderma asperellum Inoculation in Overexpressing Plants
Abstract
:1. Introduction
2. Results
2.1. PdPapARF1 Expression Is Responsive to T. asperellum Inoculation
2.2. Production of Transgenic Poplar with Modified Expression of PdPapARF1
2.3. PdPapARF1 Overexpression Promoted Growth and Adventitious Root Formation
2.4. PdPapARF1 Overexpression Altered the Response of Poplar Leaf to A. alternata Infection
2.5. PdPapARF1 Overexpression Regulated Hormone Levels in Planta
2.6. PdPapARF1 Overexpression Influenced the IAA, JA and SA Signaling Cascades in Planta
3. Discussion
4. Materials and Methods
4.1. Plant, Fungal Material, and Growth
4.2. Cloning of PdPapARF1 Sequences and Production of Transgenic Poplars
4.3. Inoculation of Plants
4.4. DAB Staining Assays, Determination of Hormone concentrations, qRT-PCR, and Statistic Analyses
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Wang, Y.-F.; Hou, X.-Y.; Deng, J.-J.; Yao, Z.-H.; Lyu, M.-M.; Zhang, R.-S. AUXIN RESPONSE FACTOR 1 Acts as a Positive Regulator in the Response of Poplar to Trichoderma asperellum Inoculation in Overexpressing Plants. Plants 2020, 9, 272. https://doi.org/10.3390/plants9020272
Wang Y-F, Hou X-Y, Deng J-J, Yao Z-H, Lyu M-M, Zhang R-S. AUXIN RESPONSE FACTOR 1 Acts as a Positive Regulator in the Response of Poplar to Trichoderma asperellum Inoculation in Overexpressing Plants. Plants. 2020; 9(2):272. https://doi.org/10.3390/plants9020272
Chicago/Turabian StyleWang, Yue-Feng, Xue-Yue Hou, Jun-Jie Deng, Zhi-Hong Yao, Man-Man Lyu, and Rong-Shu Zhang. 2020. "AUXIN RESPONSE FACTOR 1 Acts as a Positive Regulator in the Response of Poplar to Trichoderma asperellum Inoculation in Overexpressing Plants" Plants 9, no. 2: 272. https://doi.org/10.3390/plants9020272