Complete Chloroplast Genome of Rhipsalis baccifera, the only Cactus with Natural Distribution in the Old World: Genome Rearrangement, Intron Gain and Loss, and Implications for Phylogenetic Studies
Abstract
:1. Introduction
2. Results
2.1. Chloroplast Genome Organization and Features of Rhipsalis baccifera
2.2. Inversions and Rearrangements in the cp Genome of Rhipsalis baccifera
2.2.1. rbcL - atpB - atpE-trnM Inversion
2.2.2. A 19-Gene Inversion in the LSC of Rhipsalis baccifera
2.2.3. Rearrangements in the SSC Region
2.3. Amino Acid Composition of Coded Proteins in R. baccifera cp Genome
2.4. Repeats
2.5. Boundaries between IRs and the Single Copy Regions
2.6. Genome Comparison and Sequence Divergence
2.7. Phylogenetic Analysis
3. Discussion
3.1. Chloroplast Genome Organization and Features of Rhipsalis baccifera
3.2. Inversions and Rearrangements in the cp Genome of Rhipsalis baccifera
3.3. Amino Acid Composition of Coded Proteins in R. baccifera
3.4. Repeats
3.5. Boundaries between IRs and the Single Copy Regions
3.6. Genome Comparison and Sequence Divergence
3.7. Phylogenetic Analysis
4. Materials and Methods
4.1. Chloroplast Genome Sequencing and Assembly
4.2. Genome Annotation and Codon Usage Analysis
4.3. Repeat Structure and Single Sequence Repeats (SSRs) Analysis
4.4. Genome Comparison and Sequence Divergence
4.5. Phylogenetic Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Region | Size (bp) | T (U) (%) | A (%) | G (%) | C (%) | Genes |
---|---|---|---|---|---|---|
LSC | 81,459 | 32.6 | 31.4 | 17.7 | 18.3 | 84 |
SSC | 23,531 | 30.5 | 30.2 | 20.3 | 19.0 | 19 |
IRA | 8530 | 31.4 | 31.7 | 19.0 | 17.9 | 4 |
IRB | 8530 | 31.4 | 31.7 | 19.0 | 17.9 | 4 |
Total | 122,333 | 32.1 | 31.2 | 18.3 | 18.4 | 110 |
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Oulo, M.A.; Yang, J.-X.; Dong, X.; Wanga, V.O.; Mkala, E.M.; Munyao, J.N.; Onjolo, V.O.; Rono, P.C.; Hu, G.-W.; Wang, Q.-F. Complete Chloroplast Genome of Rhipsalis baccifera, the only Cactus with Natural Distribution in the Old World: Genome Rearrangement, Intron Gain and Loss, and Implications for Phylogenetic Studies. Plants 2020, 9, 979. https://doi.org/10.3390/plants9080979
Oulo MA, Yang J-X, Dong X, Wanga VO, Mkala EM, Munyao JN, Onjolo VO, Rono PC, Hu G-W, Wang Q-F. Complete Chloroplast Genome of Rhipsalis baccifera, the only Cactus with Natural Distribution in the Old World: Genome Rearrangement, Intron Gain and Loss, and Implications for Phylogenetic Studies. Plants. 2020; 9(8):979. https://doi.org/10.3390/plants9080979
Chicago/Turabian StyleOulo, Millicent Akinyi, Jia-Xin Yang, Xiang Dong, Vincent Okelo Wanga, Elijah Mbandi Mkala, Jacinta Ndunge Munyao, Victor Omondi Onjolo, Peninah Cheptoo Rono, Guang-Wan Hu, and Qing-Feng Wang. 2020. "Complete Chloroplast Genome of Rhipsalis baccifera, the only Cactus with Natural Distribution in the Old World: Genome Rearrangement, Intron Gain and Loss, and Implications for Phylogenetic Studies" Plants 9, no. 8: 979. https://doi.org/10.3390/plants9080979