Blood Cells and Redox Homeostasis in Health and Disease, 2nd Edition

A special issue of Antioxidants (ISSN 2076-3921). This special issue belongs to the section "Health Outcomes of Antioxidants and Oxidative Stress".

Deadline for manuscript submissions: 20 February 2025 | Viewed by 673

Special Issue Editors


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Guest Editor
Department of Biochemistry and Molecular Genetics, University of Colorado Denver, Anschutz Medical Campus, Aurora, CO 80045, USA
Interests: blood cells; hypoxia; metabolism; cancer metabolism; trauma; ageing; hemoglobinopathies; transfusion medicine; omics markers
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Guest Editor
Department of Biochemistry, School of Medicine, University of Patras, 26504 Patras, Greece
Interests: red blood cells; redox biology; red blood cell metabolism; storage lesion; extracellular vesicles; donor variation effect; transfusion medicine; hemoglobinopathies

Special Issue Information

Dear Colleagues,

The interplay between blood cells and redox homeostasis is crucial in maintaining cellular health and preventing disease. Redox balance, which involves the regulation of reactive oxygen species (ROS) and antioxidant defenses, is essential to proper blood cell function and overall physiological stability. Disruptions to this balance can lead to a variety of health issues, including cardiovascular diseases, cancer, and neurodegenerative disorders.

This Special Issue, "Blood Cells and Redox Homeostasis in Health and Disease, 2nd Edition", builds on the insights provided by its predecessor, the 1st Edition, which set a strong foundation by addressing key aspects of how blood cells contribute to redox regulation and the consequences of dysregulation.

This second edition will continue this exploration with updated research and new perspectives. It will delve into the latest advancements in understanding the mechanisms by which blood cells manage oxidative stress and the resulting implications for various diseases. By expanding on the groundwork laid by the first edition, this Special Issue will enhance our comprehension of blood cells' roles in redox homeostasis and will advance diagnostic and therapeutic approaches.

Prof. Dr. Angelo D'Alessandro
Dr. Alkmini T. Anastasiadi
Guest Editors

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Keywords

  • blood
  • redox status
  • antioxidants
  • oxidative stress
  • redox therapy
  • oxidative stress biomarkers

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Published Papers (1 paper)

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Research

17 pages, 1714 KiB  
Article
Modulation of Antioxidant Enzyme Expression of In Vitro Culture-Derived Reticulocytes
by Hannah D. Langlands, Deborah K. Shoemark and Ashley M. Toye
Antioxidants 2024, 13(9), 1070; https://doi.org/10.3390/antiox13091070 - 2 Sep 2024
Viewed by 575
Abstract
The regulation of reactive oxygen species (ROS) in red blood cells (RBCs) is crucial for maintaining functionality and lifespan. Indeed, dysregulated ROS occurs in haematological diseases such as sickle cell disease and β-thalassaemia. In order to combat this, RBCs possess high levels of [...] Read more.
The regulation of reactive oxygen species (ROS) in red blood cells (RBCs) is crucial for maintaining functionality and lifespan. Indeed, dysregulated ROS occurs in haematological diseases such as sickle cell disease and β-thalassaemia. In order to combat this, RBCs possess high levels of protective antioxidant enzymes. We aimed to further boost RBC antioxidant capacity by overexpressing peroxiredoxin (Prxs) and glutathione peroxidase (GPxs) enzymes. Multiple antioxidant enzyme cDNAs were individually overexpressed in expanding immortalised erythroblasts using lentivirus, including Prx isoforms 1, 2, and 6 and GPx isoforms 1 and 4. Enhancing Prx protein expression proved straightforward, but GPx overexpression required modifications. For GPx4, these modifications included adding a SECIS element in the 3’UTR, the removal of a mitochondrial-targeting sequence, and removing putative ubiquitination sites. Culture-derived reticulocytes exhibiting enhanced levels of Prx and GPx antioxidant proteins were successfully engineered, demonstrating a novel approach to improve RBC resilience to oxidative stress. Further work is needed to explore the activity of these proteins and their impact on RBC metabolism, but this strategy shows promise for improving RBC function in physiological and pathological contexts and during storage for transfusion. Enhancing the antioxidant capacity of reticulocytes has exciting promise for developing culture-derived RBCs with enhanced resistance to oxidative damage and offers new therapeutic interventions in diseases with elevated oxidative stress. Full article
(This article belongs to the Special Issue Blood Cells and Redox Homeostasis in Health and Disease, 2nd Edition)
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