Oxidative Stress in Aquaculture

A special issue of Antioxidants (ISSN 2076-3921).

Deadline for manuscript submissions: closed (15 January 2023) | Viewed by 2470

Special Issue Editor


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Guest Editor
Department of Aquatic Life and Medical Science, Sun Moon University, Asan 31460, Korea
Interests: reactive oxygen species; oxidative stress in fish; antioxidant reactions and mechanisms; toxic physiology in fish exposed to aquatic toxicants; neurotoxicity by oxidative stress

Special Issue Information

Dear Colleagues,

Various toxic substances present in water or rapid changes in the water environment cause an increase in the production of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in the aquatic organisms living in the water. Most aquatic organisms have developed antioxidant systems to protect them from free radicals, and the production of free radicals exceeding their antioxidant capacity can act as oxidative stress in aquatic organisms, which has various toxic effects on the physiology of aquatic organisms. Therefore, research on oxidative stress in aquatic organisms will be an important topic to understand the physiological and metabolic mechanisms of aquatic organisms and to evaluate the effects of various toxic substances or changes in the water environment. This research topic will evaluate the oxidative stress and physiological effects of various factors such as environmental factors, chemicals or planktonic toxins present in the aquatic environment on aquatic organisms, and discuss various toxic effects and mechanisms on aquatic organisms.

Prof. Dr. Jun-Hwan Kim
Guest Editor

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Keywords

  • reactive oxygen species
  • reactive nitrogen species
  • oxidative stress in fish
  • toxic physiology in fish exposed to aquatic toxicants

Published Papers (1 paper)

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Research

21 pages, 3066 KiB  
Article
Transcriptional Inhibition of AGPAT2 Induces Abnormal Lipid Metabolism and Oxidative Stress in the Liver of Nile Tilapia Oreochromis niloticus
by Tiantian Feng, Yifan Tao, Yue Yan, Siqi Lu, Yan Li, Xing Zhang and Jun Qiang
Antioxidants 2023, 12(3), 700; https://doi.org/10.3390/antiox12030700 - 12 Mar 2023
Cited by 3 | Viewed by 1750
Abstract
The enzyme 1-acylglycerol-3-phosphate O-acyltransferase 2 (AGPAT2) is an intermediate enzyme in triglyceride synthesis. The aim was to study the regulatory mechanism of AGPAT2 on Nile tilapia, Oreochromis niloticus. In this study, antisense RNA technology was used to knock-down AGPAT2 in Nile [...] Read more.
The enzyme 1-acylglycerol-3-phosphate O-acyltransferase 2 (AGPAT2) is an intermediate enzyme in triglyceride synthesis. The aim was to study the regulatory mechanism of AGPAT2 on Nile tilapia, Oreochromis niloticus. In this study, antisense RNA technology was used to knock-down AGPAT2 in Nile tilapia. Compared with the control groups (transfected with ultrapure water or the blank expression vector), the AGPAT2 knock-down group showed a significantly higher weight gain rate, special growth rate, visceral somatic index, and hepatopancreas somatic index; and significantly increased the total cholesterol, triglycerides, glucose, low-density lipoprotein cholesterol, and insulin levels in serum. In addition, the contents of total cholesterol and triglycerides and the abundance of superoxide dismutase, catalase, and glutathione peroxidase in the liver significantly increased, while the malondialdehyde content significantly decreased. The liver cells became severely vacuolated and accumulated lipids in the AGPAT2 knock-down group. Comparative transcriptome analyses (AGPAT2 knock-down vs. control group) revealed 1789 differentially expressed genes (DEGs), including 472 upregulated genes and 1313 downregulated genes in the AGPAT2 knock-down group. Functional analysis showed that the main pathway of differentially expressed genes enrichment was lipid metabolism and oxidative stress, such as steroid biosynthesis, unsaturated fatty acid biosynthesis, the PPAR signaling pathway, and the P53 pathway. We used qRT-PCR to verify the mRNA expression changes of 13 downstream differential genes in related signaling pathways. These findings demonstrate that knock-down of AGPAT2 in tilapia leads to abnormal lipid metabolism and oxidative stress. Full article
(This article belongs to the Special Issue Oxidative Stress in Aquaculture)
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