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Applied Sciences
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Innovative Nanobiotechnologies and Their Applications
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Innovative Nanobiotechnologies and Their Applications

A special issue of Applied Sciences (ISSN 2076-3417). This special issue belongs to the section "Nanotechnology and Applied Nanosciences".

Deadline for manuscript submissions: closed (20 January 2024) | Viewed by 5947

Special Issue Editors

Dr. Murtaza Hasan

E-Mail
Guest Editor
1. Department of Biotechnology, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan
2. School of Chemistry and Chemical Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China
Interests: green chemistry; nanobiotechnology; nanoagriculture; nanobiointeraction; nanosensor
Prof. Dr. Xugang Shu

E-Mail Website
Guest Editor
College of Chemistry and Chemical Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China
Interests: nanoagriculture; nanobiofertilizers; biochemistry

Special Issue Information

Dear Colleagues,

The emergence of nanotechnology has revoutionarized the field of sciences such as chemistry, physics, biology, and agriculture. The synthesis of nanoparticles via biological methods has further enhanced the activity of materials by adding functional molecules on surfaces of nanoparticles. The biobased synthesis of nanoparticles has improved the degradation of industrial, pharmaceutical, and agricultural waste activity. Recently, there is an urgent need to create food products by enhancing green synthesiszed nanoparticles in the following ways.

  • Nanofertilizers used in nano-bioimaging for seed germination, growth, yield, and quality;
  • Nanotechnologies used for livestock and poultry breeding to improve animals’ immunity and oxidation resistance;
  • Nanobiomaterials used for rapid detection and diagnosis, notably for clinical examination, food safety testing, and animal epidemic surveillance.

Green synthesis of nanoparticles used for antibacterial, antifungal, and antibiofilm uses, among others.

Dr. Murtaza Hasan
Prof. Dr. Xugang Shu
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Applied Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Published Papers (3 papers)

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Research

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25 pages, 4282 KiB  
Article
Optimization of Growth Conditions for Magnetospirillum magnetotacticum and Green Synthesis of Metallic Nanoparticles
by Rebekah Eleasa Sancho, Anushka Govindsamy and Karen Pillay
Appl. Sci. 2023, 13(14), 8491; https://doi.org/10.3390/app13148491 - 22 Jul 2023
Cited by 2 | Viewed by 1146
Abstract
Nanotechnology is especially useful in biotechnological and biomedical applications as nanomaterials have unique physicochemical properties. Current physical and chemical techniques used for the production of nanoparticles have various disadvantages that has led to the evaluation of biological strategies. This study focused on the [...] Read more.
Nanotechnology is especially useful in biotechnological and biomedical applications as nanomaterials have unique physicochemical properties. Current physical and chemical techniques used for the production of nanoparticles have various disadvantages that has led to the evaluation of biological strategies. This study focused on the use of a bacterial species known as Magnetospirillum magnetotacticum for the production of metallic nanoparticles. The cultivation of MTB is known to be tedious and time-consuming using the current standardized magnetic spirillum growth media (MSGM). This study explored the optimization of MSGM for improved growth and nanoparticle yield. It was found that glucose significantly improved and sustained the growth of M. magnetotacticum compared to other sole carbon sources having a sustainable OD of ~1.15. However, use of a higher concentration of sodium nitrate (40 mM) as a nitrogen source was able to significantly improve iron-containing nanoparticle yield by 1.6× with a final yield of 22 mg/50 mL when compared to the yield obtained from the MSGM original media. Growth media with a combination of glucose, sodium nitrate, ammonium sulphate and yeast extract showed the highest exponential growth of Magnetospirillum magnetotacticum compared to all other MSGM modifications with the highest OD being 1.7. Silver and gold nanoparticles were also successfully produced in addition to iron-containing nanoparticles. Overall, no direct correlation between growth and nanoparticle yield was found. Full article
(This article belongs to the Special Issue Innovative Nanobiotechnologies and Their Applications)
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11 pages, 2068 KiB  
Article
Chitosan Nanoparticles Production: Optimization of Physical Parameters, Biochemical Characterization, and Stability upon Storage
by Eduardo M. Costa, Sara Silva and Manuela Pintado
Appl. Sci. 2023, 13(3), 1900; https://doi.org/10.3390/app13031900 - 1 Feb 2023
Cited by 3 | Viewed by 1988
Abstract
Ionic gelation is among the simplest processes for the development of chitosan nanoparticles reported so far in the literature. Its one-shot synthesis process in conjunction with the mild reaction conditions required are among the main causes for its success. In this work, we [...] Read more.
Ionic gelation is among the simplest processes for the development of chitosan nanoparticles reported so far in the literature. Its one-shot synthesis process in conjunction with the mild reaction conditions required are among the main causes for its success. In this work, we sought to optimize a set of physical parameters associated with the ionic gelation process at two different pH values. Following that, the NPs’ freeze-drying and long-term storage stability were assayed, and their biocompatibility with HaCat cells was evaluated. The results show that NPs were more homogenously produced at pH 5, and that at this pH value, it was possible to obtain a set of optimum production conditions. Furthermore, of the assayed parameters, TPP addition time and overall reaction time were the parameters which had a significant impact on the produced NPs. Nanoparticle freeze-drying led to particle aggregation, and, of the cryoprotectants, assayed mannitol at 10% (w/v) presented the best performance, as the NPs were stable to freeze-drying and maintained their size and charge in the long-term stability assay. Lastly, the chitosan NPs presented no toxicity towards the HaCat cell line. Full article
(This article belongs to the Special Issue Innovative Nanobiotechnologies and Their Applications)
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Review

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14 pages, 680 KiB  
Review
Hormonal Interactions Underlying Rootstock-Induced Vigor Control in Horticultural Crops
by Faisal Hayat, Juan Li, Shahid Iqbal, Ummara Khan, Nadia Ahmed Ali, Yang Peng, Leming Hong, Sumeera Asghar, Hafiz Umer Javed, Caiqin Li, Wenpei Song, Panfeng Tu, Jiezhong Chen and Muhammad Adnan Shahid
Appl. Sci. 2023, 13(3), 1237; https://doi.org/10.3390/app13031237 - 17 Jan 2023
Cited by 6 | Viewed by 2253
Abstract
Grafting has been utilized to develop horticultural crops to increase plant vigor, yield potential, and tolerance to various environmental stresses. Grafting employs selected rootstocks of the same species or near relatives. Moreover, dwarfing is a desirable feature for intensive cultivation and efficient orchard [...] Read more.
Grafting has been utilized to develop horticultural crops to increase plant vigor, yield potential, and tolerance to various environmental stresses. Grafting employs selected rootstocks of the same species or near relatives. Moreover, dwarfing is a desirable feature for intensive cultivation and efficient orchard management. However, information on the regulatory mechanisms of rootstock-induced vigor control remains unclear. Numerous studies comprehend the physiological and molecular mechanism of rootstock control scion vigor, which has significantly improved our understanding regarding vigor control processes in apple, litchi, pear, citrus, etc. This review summarizes the current knowledge on the impact of rootstocks on scion vigor and hormonal mechanisms involved in dwarfing, such as auxin (IAA), cytokinins (CK), abscisic acid (ABA), gibberellic acids (GAs), and brassinosteroids (BR). This study will provide fascinating information for future studies on rootstock-induced dwarfing effects and accelerate the breeding of dwarfing rootstocks. Full article
(This article belongs to the Special Issue Innovative Nanobiotechnologies and Their Applications)
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