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Applied Sciences
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Bacteriophages in Therapy and Biosensor Devices
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Bacteriophages in Therapy and Biosensor Devices

A special issue of Applied Sciences (ISSN 2076-3417). This special issue belongs to the section "Applied Biosciences and Bioengineering".

Deadline for manuscript submissions: closed (25 September 2022) | Viewed by 4534

Special Issue Editors

Dr. Domenico Franco

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Guest Editor
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale Ferdinando Stagno d'Alcontres, 31, 98166 Messina, Italy
Interests: phage-display technology; drug delivery and drug targeting; nano and micro-structured systems for biosensor application; molecular biology
Special Issues, Collections and Topics in MDPI journals
Dr. Laura Maria De Plano

E-Mail Website
Guest Editor
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale Ferdinando Stagno d'Alcontres, 31, 98166 Messina, Italy
Interests: phage-display technology; drug delivery and drug targeting; nano and micro-structured systems for biosensor application; molecular biology
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Salvatore P.P. Guglielmino

E-Mail Website
Guest Editor
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale Ferdinando Stagno d'Alcontres, 31 - 98166 Messina, Italy
Interests: phage-display technology; biosensors; controlled cell adhesion on engineered surface biomaterials; IgA anti-herpes virus; arbovirus in humans and mammals

Special Issue Information

Dear Colleagues,

The search for more specific, robust and novel recognition probes is one of the major problems of innovative biosensor and therapy devices. In these applications, the bio-probes represent a new frontier to obtaining a specific and high affinity system to detect the analyte. Recent studies have employed bacteriophages (or simple phage) as a valid alternative to standard bio-probes. In this prospective, wild-type and engineered phage have been used with success as new recognition probes on the sensor substrates and/or in phage therapy to overcome the constant increase in microbial resistance to antibiotics. Moreover, phage derivatives, such as phage receptor-binding proteins (RBPs) and, most lately, phage-display peptides (PDPs), have been applied in the development of simple and economic systems that are able to detect several abiotic components, such as biotic target in diagnostic assay and in innovative targeting therapeutic devices.

The aim of this Special Issue is to provide an overview of the last generation of phage-based proof of concept, point of care and complete devices applied in the diagnostic and therapy field.

As Guest Editors of this Special Issue, we invite you to submit research articles, review articles, and short communications related to Bacteriophages in Therapy and Biosensor Devices.

Dr. Domenico Franco
Dr. Laura Maria De Plano
Prof. Dr. Salvatore P.P. Guglielmino
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Applied Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • phage-based biosensor
  • phage-based therapeutic devices
  • phage therapy
  • phage-derived antibacterials
  • phage display technology
  • receptor-binding proteins (RBPs)
  • phage-display peptides (PDPs)

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Published Papers (2 papers)

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Research

12 pages, 1852 KiB  
Article
Incidence of Phage Capsid Organization on the Resistance to High Energy Proton Beams
by Laura Maria De Plano, Letteria Silipigni, Lorenzo Torrisi, Alfio Torrisi, Mariapompea Cutroneo, Vladimir Havranek, Anna Mackova, Vincenzo Zammuto, Concetta Gugliandolo, Maria Giovanna Rizzo, Salvatore P. P. Guglielmino and Domenico Franco
Appl. Sci. 2022, 12(3), 988; https://doi.org/10.3390/app12030988 - 19 Jan 2022
Cited by 2 | Viewed by 1544
Abstract
The helical geometry of virus capsid allows simple self-assembly of identical protein subunits with a low request of free energy and a similar spiral path to virus nucleic acid. Consequently, small variations in protein subunits can affect the stability of the entire phage [...] Read more.
The helical geometry of virus capsid allows simple self-assembly of identical protein subunits with a low request of free energy and a similar spiral path to virus nucleic acid. Consequently, small variations in protein subunits can affect the stability of the entire phage particle. Previously, we observed that rearrangement in the capsid structure of M13 engineered phages affected the resistance to UV-C exposure, while that to H2O2 was mainly ascribable to the amino acids’ sequence of the foreign peptide. Based on these findings, in this work, the resistance to accelerated proton beam exposure (5.0 MeV energy) of the same phage clones was determined at different absorbed doses and dose rates. Then, the number of viral particles able to infect and replicate in the natural host, Escherichia coli F+, was evaluated. By comparing the results with the M13 wild-type vector (pC89), we observed that 12III1 phage clones, with the foreign peptide containing amino acids favorable to carbonylation, exhibited the highest reduction in phage titer associated with a radiation damage (RD) of 35 × 10−3/Gy at 50 dose Gy. On the other hand, P9b phage clones, containing amino acids unfavorable to carbonylation, showed the lowest reduction with an RD of 4.83 × 10−3/Gy at 500 dose Gy. These findings could improve the understanding of the molecular mechanisms underlying the radiation resistance of viruses Full article
(This article belongs to the Special Issue Bacteriophages in Therapy and Biosensor Devices)
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11 pages, 1721 KiB  
Article
Phage-Phenotype Imaging of Myeloma Plasma Cells by Phage Display
by Laura M. De Plano, Domenico Franco, Martina Bonsignore, Enza Fazio, Sebastiano Trusso, Alessandro Allegra, Caterina Musolino, Riccardo Cavaliere, Guido Ferlazzo, Fortunato Neri and Salvatore P. P. Guglielmino
Appl. Sci. 2021, 11(17), 7910; https://doi.org/10.3390/app11177910 - 27 Aug 2021
Cited by 3 | Viewed by 2235
Abstract
Multiple myeloma (MM) is a malignant disease based on differentiated plasma cells (PCs) in the bone marrow (BM). Flow cytometry and fluorescence microscopy, used to identify a large combination of clusters of differentiation (CDs), are applied for MM immunophenotyping. However, due to the [...] Read more.
Multiple myeloma (MM) is a malignant disease based on differentiated plasma cells (PCs) in the bone marrow (BM). Flow cytometry and fluorescence microscopy, used to identify a large combination of clusters of differentiation (CDs), are applied for MM immunophenotyping. However, due to the heterogeneous MM immunophenotypes, more antibody panels are necessary for a preliminary diagnosis and for the monitoring of minimal residual disease (MRD). In this study, we evaluated the use of phage clones as probes for the identification of several PCs immunophenotypes from MM patients. First, A 9-mer M13-pVIII phage display library was screened against an MM.1 cells line to identify peptides that selectively recognize MM.1 cells. Then, the most representative phage clones, with amino acid sequences of foreign peptides closer to the consensus, were labelled with isothiocyanate of fluorescein (FITC) and were used to obtain a fluorescent signal on cells in ex-vivo samples by fluorescence microscopy. Selected phage clones were able to discriminate different MM immunophenotypes from patients related to CD45, CD38, CD56, and CD138. Our results highlight the possibility of using a phage-fluorescence probe for the simultaneous examination of the presence/absence of CDs associated with disease usually detected by combination of anti-CD antibodies. The design of a multi-phage imaging panel could represent a highly sensitive approach for the rapid detection of immunophenotype subtypes and the subsequent characterization of patient disease status. Full article
(This article belongs to the Special Issue Bacteriophages in Therapy and Biosensor Devices)
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