Ultrasensitive Biosensors and Bioassays for Real-Time Monitoring of Food Contaminants

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Nano- and Micro-Technologies in Biosensors".

Deadline for manuscript submissions: 31 December 2025 | Viewed by 1520

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Light, Nanomaterials, Nanotechnologies (L2n) Laboratory, CNRS UMR 7076, University of Technology of Troyes, 12 Rue Marie Curie CS 42060, 10004 Troyes, France
Interests: nanoparticles; multi-analyte biosensing platforms; pollutants toxicity to living cells
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Special Issue Information

Dear Colleagues,

Food safety is a permanent and global concern to guarantee human health. Over the years, several analytical methods have been proposed/improved for the specific and selective detection of different classes of contaminants (pesticides, herbicides, etc.) necessary for the life cycle of foods based on complex seed preparation protocols followed by systematic chemical treatments of plants/soils/groundwater and optimal storage conditions. Unfortunately, there are growing concerns regarding abnormal amounts of some contaminants absorbed by fresh and dried foods often found in open and closed markets. Therefore, more sensitive and easier-to-use techniques are needed to monitor trace chemicals in fresh and processed food products by various industries and/or consumers.

This Special Issue aims to gather the latest developments in the use of nanomaterials in the development of biosensors and biological assays, with a particular focus on the improved robustness of different sensing platforms for real-time identification of environmental contaminants present in air, soil, and water.

Dr. Rodica Elena Ionescu
Guest Editor

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Keywords

  • synthesis of nanomaterials
  • food contaminants
  • real-time investigations
  • biosensors
  • bioassays
  • single use testing kits

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Published Papers (2 papers)

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Research

11 pages, 1405 KiB  
Article
Enhancing Biocide Safety of Milk Using Biosensors Based on Cholinesterase Inhibition
by Lynn Mouawad, Georges Istamboulie, Gaëlle Catanante and Thierry Noguer
Biosensors 2025, 15(1), 26; https://doi.org/10.3390/bios15010026 - 6 Jan 2025
Viewed by 475
Abstract
A sensitive and reliable electrochemical biosensor for the detection of benzalkonium chloride (BAC) and didecyldimethylammonium chloride (DDAC), the most commonly used disinfectant biocides in the agri-food industry, is described. Acetylcholinesterase from Drosophila melanogaster (DM AChE) and butyrylcholinesterase from horse serum (BChE) were immobilized [...] Read more.
A sensitive and reliable electrochemical biosensor for the detection of benzalkonium chloride (BAC) and didecyldimethylammonium chloride (DDAC), the most commonly used disinfectant biocides in the agri-food industry, is described. Acetylcholinesterase from Drosophila melanogaster (DM AChE) and butyrylcholinesterase from horse serum (BChE) were immobilized by entrapment in a photocrosslinkable polymer on the surface of carbon screen-printed electrodes. Preliminary tests conducted in phosphate buffer showed limits of detection (LODs) of 0.26 µM for BAC using the BChE-based sensor and 0.04 µM for DDAC using the DM AChE sensor. These performances comply with the European regulation for dairy products, which sets a maximum allowable concentration of 0.28 µM for biocides. However, when tested directly in milk samples, a dramatic decrease in the sensitivity of both sensors towards BAC and DDAC biocides was reported. To overcome this problem, a simple liquid–liquid extraction was necessary prior to biosensor measurements, ensuring that the biosensors met European regulatory standards and provided an unbiased response. Full article
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14 pages, 2747 KiB  
Article
Electrochemical Magnetic Immunoassay for the Determination of the Fish Allergen β-Parvalbumin
by José Pedro Rocha, Maria Freitas, Dulce Geraldo, Fátima Bento, Cristina Delerue-Matos and Henri P. A. Nouws
Biosensors 2024, 14(12), 639; https://doi.org/10.3390/bios14120639 - 23 Dec 2024
Viewed by 609
Abstract
β-parvalbumin (β-PV) is the primary fish allergen responsible for most allergic reactions in individuals sensitive to fish. To ensure food safety, a sandwich-based magnetic immunoassay was developed using maleimide-functionalized magnetic beads (NH-MBs). Specific anti-β-PV antibodies were immobilized on these MBs, and a screen-printed [...] Read more.
β-parvalbumin (β-PV) is the primary fish allergen responsible for most allergic reactions in individuals sensitive to fish. To ensure food safety, a sandwich-based magnetic immunoassay was developed using maleimide-functionalized magnetic beads (NH-MBs). Specific anti-β-PV antibodies were immobilized on these MBs, and a screen-printed carbon electrode was employed as the electrochemical transducer. A linear concentration range from 10 to 1000 ng/mL, a limit of detection of 1.8 ng/mL, and a limit of quantification of 7.1 ng/mL were achieved. Nineteen commercial food samples were analyzed to assess the potential of the sensor for routine use in food quality control. Important factors such as protein source and food processing (e.g., boiling, grilling, and frying) and preservation (e.g., in oil, and vacuum) were evaluated. The validated results confer the usefulness of the assay for food quality control. Full article
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