Flow Cytometry of Hematological Malignancies

A special issue of Cancers (ISSN 2072-6694). This special issue belongs to the section "Methods and Technologies Development".

Deadline for manuscript submissions: 1 December 2024 | Viewed by 1125

Special Issue Editors


E-Mail Website
Guest Editor
UT Southwestern Medical Center, Dallas, TX 75390, USA
Interests: flow cytometry; hematopathology (leukemias and lymphomas)

E-Mail Website
Guest Editor
UT Southwestern Medical Center, Dallas, TX 75390, USA
Interests: pathology and diagnosis of hematologic cancers

Special Issue Information

Dear Colleagues,

Flow cytometry (FC) is a primary tool for establishing immunophenotypes of cell populations to identify and characterize hematolymphoid disorders. Its analytic approach is evolving to facilitate efficient and accurate analysis in a world with ever-changing challenges and opportunities created by novel therapeutic approaches and technological advancements.

In this Special Issue, we will present current FC diagnostic approaches to various hematologic malignancies (including lymphoblastic leukemia, lymphoma, plasma cell neoplasm, and acute myeloid leukemia) and strategies used in minimal/measurable residual disease analysis and post-immunotherapy settings [including chimeric antigen receptor-T cell therapy (CAR-T) and bispecific T-cell engager (BiTE) monoclonal antibodies]. Lastly, future directions of the field will be examined, including the incorporation of spectral flow cytometry into clinical practice and the increased use of machine learning for flow cytometry analysis.

Prof. Dr. Weina Chen
Prof. Dr. Franklin S. Fuda
Guest Editors

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Keywords

  • flow cytometry
  • minimal or measurable residual disease (MRD)
  • immunotherapy
  • leukemia
  • lymphoma
  • myeloma
  • chimeric antigen receptor T cells (CAR-T)
  • bispecific T-cell engager (BiTE)
  • B-cell maturation antibody (BCMA)
  • spectral flow cytometry
  • machine learning

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Published Papers (1 paper)

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Review

11 pages, 4061 KiB  
Review
Flow Cytometry Profiling of Plasmacytoid Dendritic Cell Neoplasms
by Siba El Hussein and Wei Wang
Cancers 2024, 16(11), 2118; https://doi.org/10.3390/cancers16112118 - 1 Jun 2024
Viewed by 719
Abstract
In this review, we aim to provide a summary of the diverse immunophenotypic presentations of distinct entities associated with plasmacytoid dendritic cell (pDC) proliferation. These entities include the following: (1) blastic plasmacytoid dendritic cell neoplasm (BPDCN); (2) mature pDC proliferation (MPDCP), most commonly [...] Read more.
In this review, we aim to provide a summary of the diverse immunophenotypic presentations of distinct entities associated with plasmacytoid dendritic cell (pDC) proliferation. These entities include the following: (1) blastic plasmacytoid dendritic cell neoplasm (BPDCN); (2) mature pDC proliferation (MPDCP), most commonly seen in chronic myelomonocytic leukemia (CMML); and (3) myeloid neoplasms with pDC differentiation, in which pDCs show a spectrum of maturation from early immature pDCs to mature forms, most commonly seen in acute myeloid leukemia (pDC-AML). Our aim is to provide a flow cytometry diagnostic approach to these distinct and sometimes challenging entities and to clarify the immunophenotypic spectrum of neoplastic pDCs in different disease presentations. In this review, we also cover the strategies in the evaluation of residual disease, as well as the challenges and pitfalls we face in the setting of immune and targeted therapy. The differential diagnosis will also be discussed, as blasts in some AML cases can have a pDC-like immunophenotype, mimicking pDCs. Full article
(This article belongs to the Special Issue Flow Cytometry of Hematological Malignancies)
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