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Applications of Nuclear Magnetic Resonance (NMR), Mass Spectroscopy (MS), and...
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Applications of Nuclear Magnetic Resonance (NMR), Mass Spectroscopy (MS), and GC-MS to Food Science

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Food Engineering and Technology".

Deadline for manuscript submissions: closed (24 October 2024) | Viewed by 1923

Special Issue Editor

Prof. Dr. Hesham El-Seedi

E-Mail Website
Guest Editor
1. International Joint Research Laboratory of Intelligent Agriculture and Agri-Products Processing, Jiangsu Education Department, Zhenjiang 212013, China
2. Pharmacognosy Group, Department of Pharmaceutical Biosciences, Uppsala University, Biomedical Centre, Box 574, 751 23 Uppsala, Sweden
Interests: isolation; biologically active compounds; medicinal plants; anti-inflammatory; anti-cancer; insecticidal; anti-microbial
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS) are pivotal analytical methods extensively employed in food science applications. By combining their capabilities, they offer complementary insights, facilitating a comprehensive analysis of food samples. In the realm of food science, NMR and MS are indispensable tools for evaluating quality, detecting adulterants and identifying contaminants such as pesticides and mycotoxins. Their versatility and sensitivity make them invaluable for studying various aspects of food, including composition, safety and functionality, thereby significantly contributing to the enhancement of food products. Furthermore, NMR and MS are crucial for investigating the effects of preservation methods on food quality, monitoring reactions, identifying degradation products and assessing the impact of storage conditions. These techniques are instrumental and non-destructive tools in flavor and aroma analysis, enabling the identification and quantification of volatile compounds that influence sensory attributes. Moreover, they support metabolomics studies, facilitating the analysis of metabolic profiles in food samples to understand composition, biochemical pathways and health implications. Their multifaceted applications underscore their importance in food science and its various research areas.

Prof. Dr. Hesham El-Seedi
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Foods is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • nutritional analysis
  • metabolomics and food omics
  • food processing and preservation
  • food safety
  • food quality and authenticity
  • food structure
  • quantitative analysis
  • structural elucidation

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Published Papers (2 papers)

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Research

20 pages, 7278 KiB  
Article
Extraction, Identification, and Antioxidant Activity of Flavonoids from Hylotelephium spectabile (Boreau) H. Ohba
by Na Li, Xiao Wu, Qin Yin, Zeng Dong, Lele Zheng, Yihui Qian, Yulu Sun, Ziping Chen and Kefeng Zhai
Foods 2024, 13(17), 2652; https://doi.org/10.3390/foods13172652 - 23 Aug 2024
Cited by 2 | Viewed by 956
Abstract
The extraction of total flavonoids from Hylotelephium spectabile (Boreau) H. Ohba (H. spectabile) leaves was studied through the use of a double enzyme-assisted ultrasonic method, and the extraction process was optimized using the Box–Behnken design. Eight different macroporous resins were screened [...] Read more.
The extraction of total flavonoids from Hylotelephium spectabile (Boreau) H. Ohba (H. spectabile) leaves was studied through the use of a double enzyme-assisted ultrasonic method, and the extraction process was optimized using the Box–Behnken design. Eight different macroporous resins were screened for purification in single-factorial experiments, and the flavonoid compounds in the extract of H. spectabile leaves were identified using HPLC-MS. Through the evaluation of the total reducing capacity and capacity for reducing 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH), hydroxyl radicals (·OH), and 2,2’-biazobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), the in vitro antioxidant activities of the crude extracts of the total flavonoids and purified total flavonoids of H. spectabile leaves were investigated. The results showed that the most efficient conditions for flavonoid extraction were an ultrasonic extraction time of 60 min, an ethanol concentration of 35%, a liquid-to-material ratio of 20:1 mL/g, and an amount of enzyme (cellulose/pectinase = 1:1) of 1.5%, forming H. spectabile powder. Under these conditions, the total flavonoid extraction rate in the H. spectabile leaf extract was 4.22%. AB-8 resin showed superior performance in terms of purification, and the optimal adsorption and desorption times were 1.5 h and 3 h, respectively. The recommended parameters for purification included a liquid volume of 5.5 BV, a flow rate of 1.2 BV/min, a pH of 5, and a concentration of 0.8 mg/mL. The observed order for reducing capacity was ascorbic acid (VC) > rutin > purified total flavonoids > crude extract of total flavonoids. The purified total flavonoid extract from H. spectabile showed a good scavenging ability against DPPH, ·OH, and ABTS·+, suggesting strong antioxidant activity. Therefore, this study can serve as technical support and reference data for the further development and utilization of H. spectabile resources. Full article
(This article belongs to the Special Issue Applications of Nuclear Magnetic Resonance (NMR), Mass Spectroscopy (MS), and GC-MS to Food Science)
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16 pages, 3397 KiB  
Article
Comparative 1H NMR-Based Metabolomics of Traditional Landrace and Disease-Resistant Chili Peppers (Capsicum annuum L.)
by Gi-Un Seong, Dae-Yong Yun, Dong-Hyeok Shin, Jeong-Seok Cho, Gyuseok Lee, Jeong Hee Choi, Kee-Jai Park, Kyung-Hyung Ku and Jeong-Ho Lim
Foods 2024, 13(13), 1966; https://doi.org/10.3390/foods13131966 - 21 Jun 2024
Cited by 3 | Viewed by 1190
Abstract
Chili peppers (Capsicum annuum L.) are economically valuable crops belonging to the Solanaceae family and are popular worldwide because of their unique spiciness and flavor. In this study, differences in the metabolomes of landrace (Subicho) and disease-resistant pepper cultivars (Bulkala and Kaltanbaksa) [...] Read more.
Chili peppers (Capsicum annuum L.) are economically valuable crops belonging to the Solanaceae family and are popular worldwide because of their unique spiciness and flavor. In this study, differences in the metabolomes of landrace (Subicho) and disease-resistant pepper cultivars (Bulkala and Kaltanbaksa) widely grown in Korea are investigated using a 1H NMR-based metabolomics approach. Specific metabolites were abundant in the pericarp (GABA, fructose, and glutamine) and placenta (glucose, asparagine, arginine, and capsaicin), highlighting the distinct physiological and functional roles of these components. Both the pericarp and placenta of disease-resistant pepper cultivars contained higher levels of sucrose and hexoses and lower levels of alanine, proline, and threonine than the traditional landrace cultivar. These metabolic differences are linked to enhanced stress tolerance and the activation of defense pathways, imbuing these cultivars with improved resistance characteristics. The present study provides fundamental insights into the metabolic basis of disease resistance in chili peppers, emphasizing the importance of multi-resistant varieties to ensure sustainable agriculture and food security. These resistant varieties ensure a stable supply of high-quality peppers, contributing to safer and more sustainable food production systems. Full article
(This article belongs to the Special Issue Applications of Nuclear Magnetic Resonance (NMR), Mass Spectroscopy (MS), and GC-MS to Food Science)
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