RNA-Targeting CRISPR Systems
A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Biochemistry".
Deadline for manuscript submissions: closed (20 August 2023) | Viewed by 13095
Special Issue Editor
Interests: CRISPR-Cas; microbiology; next-generation sequencing; bioinformatics data analysis
Special Issues, Collections and Topics in MDPI journals
Special Issue Information
Dear Colleagues,
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated (Cas) systems are mechanisms of acquired defense in prokaryotes. CRISPR arrays are DNA stretches in the prokaryote genomic that store short genomic sequences (spacers) of bacteriophages from past attacks. Upon a new phage challenge, these spacers are spliced and the resulting RNA acts as a guide to binding to the complementary sequence in the attacking phage; the associated Cas effector then cleaves the phage DNA and the viral threat is neutralized.
Since their discovery, CRISPR-Cas systems have been engineered into the most promising genome editing tool: a programmable effector deliverable anywhere in a genomic sequence to modify a nucleotide sequence.
Initially, it seemed that CRISPR-Cas systems were capable of targeting exclusively DNA. Later it has been discovered that they can also cleave RNA. The main advantage of RNA-targeting CRISPR-Cas is that they act on a transient entity, therefore allowing modifications that might otherwise be deleterious or lethal if applied to the genome.
This Special Issue will focus on the growing number of applications of RNA-targeting CRISPR-Cas-based editing, such as transcriptional repression, RNA editing, guided isoform splicing, nucleic acid detection, expression regulation, and viral resistance. Additional suggested topics include RNA-targeting CRISPR systems structures and mechanisms of action, off-target activity in CRISPR-mediated RNA cleavage, and in silico methods for the discovery of new systems.
Dr. Giancarlo Russo
Guest Editor
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Keywords
- CRISPR-Cas
- Cas13
- Cmr
- Csm
- RNA editing
- RNA knockdown
- CRISPRi
- off-target effects
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