Fruit Breeding and Breeding

A special issue of Life (ISSN 2075-1729). This special issue belongs to the section "Plant Science".

Deadline for manuscript submissions: closed (23 June 2023) | Viewed by 3286

Special Issue Editors


E-Mail Website
Guest Editor
Department of Horticulture, Faculty of Agriculture, Cukurova University, Adana, Turkey
Interests: fruits; pomology; biodiversity; genetics; berry fruits; fruit quality

E-Mail Website
Guest Editor
Department of Agricultural, Food and Environmental Sciences, Università Politecnica delle Marche, Via Brecce Bianche 10, 60131 Ancona, Italy
Interests: fruits; breeding; diversity; traditional uses; ethnoveterinary; characterization of phytochemicals
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Fruits are an excellent source of essential vitamins and minerals, and they are high in fiber. Fruits also provide a wide range of health-boosting antioxidants, including flavonoids. Fruit breeding, the genetic improvement of fruit crops, has an ancient tradition that shares links with the domestication process. Millennial genetic improvement has been achieved by grower selection, first from natural seedling populations and then from seedlings that occurred naturally in grower fields with desirable genotypes fixed by vegetative propagation. In the middle of the 19th century, hybridization between elite genotypes was achieved, followed by the selection of segregating progeny. Conventional fruit breeding is based on the continuous selection of superior phenotypes from genetically variable populations, using cycles of hybridization and selection and special techniques, such as mutation induction, interspecific hybridization, and backcross breeding. Recently, biotechnology techniques have been employed, including in vitro propagation, embryo rescue, protoplast fusion, marker-assisted selection, and transgenesis. The Special Issue ‘Fruit Breeding and Breeding’ aims to highlight the latest improved methods and techniques used to investigate the research questions in genetic studies on fruit breeding in the current genomic era. Topics of interest include, but are not limited to, the following:

Genetic studies on classical and modern fruit breeding;

  • In vitro propagation;
  • Transgenesis;
  • Breeding methods;
  • General and specific breeding;
  • Genomics, transcriptomics and metabolomics;
  • Population genetics, domestication, adaptation and selection;
  • Marker-assisted selection;
  • Mutagenesis in fruit breeding, etc.;
  • Functional foods;
  • Pre-breeding studies.

This research topic includes technologies and up-to-date methods that help to advance science.

Prof. Dr. Nesibe Ebru Kafkas
Dr. Luca Mazzoni
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Life is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • fruits
  • breeding methods
  • traditional and modern breeding
  • domestication of fruits
  • molecular characterization
  • marker-assisted selection
  • pre-breeding studies

Published Papers (1 paper)

Order results
Result details
Select all
Export citation of selected articles as:

Research

12 pages, 2511 KiB  
Article
Development of Molecular Marker Linked to Seed Hardness in Pomegranate Using Bulked Segregant Analysis
by Keziban Yazıcı, Betül Gönülkırmaz and Mehtap Şahin Çevik
Life 2023, 13(5), 1123; https://doi.org/10.3390/life13051123 - 1 May 2023
Viewed by 1305
Abstract
The pomegranate (Punica granatum L.) is one of the fruit species with the oldest cultural history. There are many traits to determine the quality of pomegranate fruits. Among them, soft-seeded feature of pomegranate fruit is important trait for the market value of [...] Read more.
The pomegranate (Punica granatum L.) is one of the fruit species with the oldest cultural history. There are many traits to determine the quality of pomegranate fruits. Among them, soft-seeded feature of pomegranate fruit is important trait for the market value of the fruit. For this reason, the demand for pomegranate varieties with soft seeds has been increasing, especially in recent years. In this study, molecular markers associated with seed hardness were developed to distinguish pomegranate cultivars with soft-seeded feature based on genomic DNA at the early stages of the pomegranate breeding process. For this purpose, pomegranate genotypes and/or cultivars from the population involved in reciprocal crosses of hard-seeded Ernar, medium-hard-seeded Hicaznar, and soft-seeded Fellahyemez cultivars were grouped as soft-seeded or hard-seeded. Further, leaf samples were collected from individuals belonging to each group. Then, the genomic DNA was isolated from each plant separately, and equal amount of genomic DNA from individuals with the similar seed hardness were mixed for bulked segregant analysis (BSA). The bulked genomic DNAs of opposite characters were analyzed by polymerase chain reaction (PCR) using random decamer primers to develop random amplified polymorphic DNA (RAPD) markers associated with soft-seeded or hard-seeded pomegranates. A total of three RAPD markers were determined to distinguish the individuals having soft- or hard-seeded pomegranate genotypes and/or cultivars. As a result of the comparison of the DNA sequences of these RAPD markers, insertion-deletions (inDels) primers were designed to developed and validate a PCR assay to distinguish the soft- and hard-seeded pomegranate genotypes/cultivars from each other. The molecular markers developed in this study will enable us to distinguish soft-seeded pomegranate types easily in a short time at the early stages of the pomegranate breeding programs. Full article
(This article belongs to the Special Issue Fruit Breeding and Breeding)
Show Figures

Figure 1

Back to TopTop