Biotechnological Potential of Extreme Microbial Cell Factories for Sustainable Process

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Environmental Microbiology".

Deadline for manuscript submissions: closed (31 December 2023) | Viewed by 2739

Special Issue Editors


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Guest Editor
Institute of Biomolecular Chemistry, National Research Council (CNR-ICB), Via Campi Flegrei 34, 80078 Pozzuoli, Naples, Italy
Interests: extremophiles; exopolysaccharides; extremozymes; valorization of vegetable biomass; taxonomy of extremophiles
Special Issues, Collections and Topics in MDPI journals

E-Mail Website
Guest Editor
National Research Council (CNR-ICB), Institute of Biomolecular Chemistry, Via Campi Flegrei 34, 80078 Pozzuoli, Naples, Italy
Interests: extremophilic microorganisms; extremozymes; exopolysaccharides; biotransformation; waste biomass valorization
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Extreme microorganisms are deeply specialized forms of life that colonize peculiar environmental niches. Those areas seem incompatible with life because they are affected by hostile physicochemical parameters. High pressure, absence of oxygen, extreme pH and temperature, salt saturation and their concomitant presence are just a few examples of stress conditions that can be found in habitats where extremophiles and poly-extremophiles proliferate. The stability of nucleic acids, cellular structures and metabolic pathways, together with the targeted synthesis of molecules, make these organisms perfectly adapted to such environments. Microbial technologies exploit cells and their biomolecules to improve industrial processes, with the purpose of making them more efficient, economical and sustainable. The aim of this Special Issue is to collect reviews and research papers emphasising the value added by extremophilic microorganisms and their products, in order to set up eco-compatible and health-safe production processes.

Dr. Annarita Poli
Dr. Ilaria Finore
Guest Editors

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Published Papers (1 paper)

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Research

8 pages, 923 KiB  
Communication
Optimization of Transposon Mutagenesis Methods in Pseudomonas antarctica
by Sangha Kim and Changhan Lee
Microorganisms 2023, 11(1), 118; https://doi.org/10.3390/microorganisms11010118 - 1 Jan 2023
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Abstract
Pseudomonas is a widespread genus in various host and environmental niches. Pseudomonas exists even in extremely cold environments such as Antarctica. Pseudomonas antarctica is a psychrophilic bacterium isolated from Antarctica. P. antarctica is also known to produce antimicrobial substances. Although P. antarctica can [...] Read more.
Pseudomonas is a widespread genus in various host and environmental niches. Pseudomonas exists even in extremely cold environments such as Antarctica. Pseudomonas antarctica is a psychrophilic bacterium isolated from Antarctica. P. antarctica is also known to produce antimicrobial substances. Although P. antarctica can provide insight into how bacteria have adapted to low temperatures and has significant potential for developing novel antimicrobial substances, progress in genetic and molecular studies has not been achieved. Transposon mutagenesis is a useful tool to screen genes of interest in bacteria. Therefore, we attempted for the first time in P. antarctica to generate transposon insertion mutants using the transfer of a conjugational plasmid encoding a transposon. To increase the yield of transposon insertion mutants, we optimized the methods, in terms of temperature for conjugation, the ratio of donor and recipient during conjugation, and the concentration of antibiotics. Here, we describe the optimized methods to successfully generate transposon insertion mutants in P. antarctica. Full article
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