The 15th Anniversary of Pharmaceutics—Aptamers as Novel Therapeutics

A special issue of Pharmaceutics (ISSN 1999-4923). This special issue belongs to the section "Biologics and Biosimilars".

Deadline for manuscript submissions: 31 August 2024 | Viewed by 871

Special Issue Editors


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Guest Editor
Department of Orthopedics, University Medical Center, Heidelberglaan 100, P.O. Box 85500, 3508 GA Utrecht, The Netherlands
Interests: biomaterials-based local delivery approaches for regeneration and treatment of inflammation
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Co-Guest Editor
Department of Orthopedics, University Medical Center Utrecht, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
Interests: osteoarthritis; senescence; regenerative medicine; aptamers

Special Issue Information

Dear Colleague,

Aptamers are nucleotide-based molecules that can be used as antibody alternatives in analytical, diagnostic, and therapeutic applications. Aptamers can be selected from large libraries to bind a specific target, including targets that are traditionally difficult for other affinity reagents, such as antibodies. Together with their stability, the ease and reproducibility of synthesis render this molecule class the therapeutic agent of the future.

This Special Issue encompasses translational research into the effectivity as well as promises of, and hurdles to be taken for, aptamers as targeting ligands and therapeutic molecules in a variety of diseases.

Dr. Laura B. Creemers
Dr. Jaqueline Lourdes Rios
Guest Editors

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Published Papers (1 paper)

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Research

23 pages, 4798 KiB  
Article
Imiquimod-Loaded Nanosystem for Treatment Human Papillomavirus-Induced Lesions
by Izamara Maocha, Beatriz Rosado, Jéssica Lopes-Nunes, Melanie Lopes, Joana Rolo, Bruno Pires, Eugénia Gallardo, Ana Palmeira-de-Oliveira, José Martinez-de-Oliveira, Rita Palmeira de Oliveira, Rui Medeiros and Carla Cruz
Pharmaceutics 2024, 16(7), 864; https://doi.org/10.3390/pharmaceutics16070864 - 27 Jun 2024
Cited by 1 | Viewed by 556
Abstract
Human papillomavirus (HPV)-associated cervical cancer is the most common cancer among women worldwide. The treatment options are strongly related to increased infertility in women. Imiquimod (IQ) is an imidazoquinoline, which has proven antiviral effects against persistent HPV infection by activating immune cells via [...] Read more.
Human papillomavirus (HPV)-associated cervical cancer is the most common cancer among women worldwide. The treatment options are strongly related to increased infertility in women. Imiquimod (IQ) is an imidazoquinoline, which has proven antiviral effects against persistent HPV infection by activating immune cells via Toll-like receptors 7/8 when formulated in carriers, like nanogels, for topical use. An effective alternative to conventional therapies is the nanoparticle drug delivery system. We studied lipidic nanoparticles with IQ (Lipo IQ) and functionalized them with a DNA aptamer, AT11 (Lipo IQ AT11), to improve the selectivity for cervical cancer cells combined with the efficacy of essential oils. The formulations showed that the physicochemical properties are adequate for vaginal drug delivery and have antimicrobial activity at higher concentrations (with MIC50 starting from 0.625%). The final formulations exhibited cytotoxicity in cancer cells, enhanced by essential oils without affecting healthy cells, resulting in less than 10% cell viability in HeLa cells and over 60% in NHDF cells. Essential oils potentiate Lipo IQ’s effectiveness, while AT11 increases the selectivity for cervical cancer cells. As suggested by the results of the permeation assay, the formulations were internalized by the cancer cells. Overall, the obtained results suggested that the synergistic effect of the essential oils and the nanosystem potentiate the cytotoxic effect of Lipo IQ and that Lipo IQ AT11 promotes selectivity towards cancer cells. Full article
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