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Extraction, Composition and Comparison of Plant Volatile Components
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Extraction, Composition and Comparison of Plant Volatile Components

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Phytochemistry".

Deadline for manuscript submissions: 20 May 2025 | Viewed by 1982

Special Issue Editors

Prof. Dr. Valerija Dunkić

E-Mail Website
Guest Editor
Department of Biology, Faculty of Science, University of Split, 21000 Split, Croatia
Interests: aromatic plants; medicinal plants; essential oils; hydrolates; chromatographic techniques; biological activities
Special Issues, Collections and Topics in MDPI journals
Dr. Marija Nazlić

E-Mail Website
Guest Editor
Department of Biology, Faculty of Science, University of Split, 21000 Split, Croatia
Interests: aromatic plants; medicinal plants; plant extracts, phytochemicals; essential oils; hydrosols; natural product chemistry; antioxidants; extraction techniques; chromatographic techniques; biological activity of plant extracts; chemophenetic markers

Special Issue Information

Dear Colleagues,

The isolation of plant volatiles, which are important specialized metabolites, can be accomplished by classical and green extraction techniques. Classical extraction techniques include steam distillation, hydrodiffusion, hydrodistillation, destructive distillation, and cold pressing. Green extraction techniques include turbo distillation, ultrasound-assisted extraction, microwave-assisted extraction, and instant controlled pressure drop technology. Depending on the isolation technique, the compositions of the substances extracted from the same plant material may vary. This depends on the duration of extraction, temperature, pressure, and quality of the plant material. Green extraction requires less time and water than traditional extraction.

The aim of this Special Issue of Plants is to publish studies dealing with the effects of different extraction procedures and their impact on the qualitative and quantitative composition of plant isolates. The research can describe the importance of various free volatile compounds to quality parameters of plants. Research comparing different biological potential of plant extracts obtained by different extraction methods is also useful.

Prof. Dr. Valerija Dunkić
Dr. Marija Nazlić
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • free volatile compounds

  • essential oil

  • extracts

  • hydrosols

  • isolates

  • classical extraction

  • green extraction

  • hydrodistillation

  • advanced modern extraction techniques

  • microwave-assisted extraction

  • chromatography

  • terpenes

  • biological activity

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Published Papers (2 papers)

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Research

22 pages, 2900 KiB  
Article
Seed Characteristics and Terpene Variability of Mediterranean Fir Species (Abies nebrodensis, A. pinsapo, and A. alba)
by Waed Tarraf, Tolga İzgü, Carla Benelli, Gabriele Cencetti, Marco Michelozzi and Alfonso Crisci
Plants 2025, 14(6), 892; https://doi.org/10.3390/plants14060892 - 12 Mar 2025
Viewed by 411
Abstract
Most fir species in the Mediterranean have small to medium-sized distributions, are often endemic and endangered, and are mainly found in relict areas, except for Abies alba. The IUCN Red List of Threatened Species identified Abies nebrodensis as the rarest conifer in the [...] Read more.
Most fir species in the Mediterranean have small to medium-sized distributions, are often endemic and endangered, and are mainly found in relict areas, except for Abies alba. The IUCN Red List of Threatened Species identified Abies nebrodensis as the rarest conifer in the world, with only 30 adult trees remaining. Additionally, Abies pinsapo is threatened and limited to five fragmented locations in Spain and Morocco. This study aimed to characterize the seed terpene profiles of Mediterranean Abies species, such as A. nebrodensis, A. pinsapo, and A. alba, since morphological results showed minimal variation among the Abies populations examined. Terpenes were extracted using n-heptane and then analyzed by GC-MS. The chemical composition revealed the dominance of limonene and α-pinene as the main monoterpenes in all the species, while A. nebrodensis reported the considerable presence of germacrene D-4-ol and selina-6-en-4-ol as sesquiterpenes. The relative contents of most of the terpenes were significantly different among the species, and subsequent statistical multivariate analysis showed clear discrimination among three distinct groups. These results confirmed the suitability of the terpene profile as a potential tool to study chemotaxonomic differences between species from the same family. Moreover, the compounds identified can be interesting for further studies on plant defense against biotic stress to reduce the risk of species extinction caused by pests and diseases. Full article
(This article belongs to the Special Issue Extraction, Composition and Comparison of Plant Volatile Components)
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18 pages, 3450 KiB  
Article
Analysis of the Volatile and Enantiomeric Compounds Emitted by Plumeria rubra L. Flowers Using HS-SPME–GC
by James Calva, Jhoyce Celi and Ángel Benítez
Plants 2024, 13(17), 2367; https://doi.org/10.3390/plants13172367 - 25 Aug 2024
Viewed by 1021
Abstract
The volatile components emitted by fresh aromatic flowers of Plumeria rubra L., harvested in southern Ecuador during three different months were determined to evaluate the fluctuation of secondary metabolites. The volatile compounds were analyzed using headspace solid-phase microextraction (HS-SPME) followed by gas chromatography [...] Read more.
The volatile components emitted by fresh aromatic flowers of Plumeria rubra L., harvested in southern Ecuador during three different months were determined to evaluate the fluctuation of secondary metabolites. The volatile compounds were analyzed using headspace solid-phase microextraction (HS-SPME) followed by gas chromatography coupled to mass spectrometry (GC–MS) and a flame ionization detector (GC–FID) using two types of columns: a non-polar (DB-5ms) and polar column (HP-INNOWax). The principal chemical groups were hydrocarbon sesquiterpenes (43.5%; 40.0%), oxygenated sesquiterpenes (23.4%; 26.4%), oxygenated monoterpenes (14.0%; 11.2%), and hydrocarbon monoterpenes (12.7%; 9.3%). The most representative constituents were (E,E)-α-Farnesene (40.9–41.2%; 38.5–50.6%), (E)-nerolidol (21.4–32.6%; 23.2–33.0%), (E)-β-ocimene (4.2–12.5%; 4.5–9.1%), (Z)-dihydro-apofarnesol (6.5–9.9%; 7.6–8.6%), linalool (5.6–8.3%; 3.3–7.8%), and perillene (3.1–5.9%; 3.0–3.2%) in DB-5ms and HP-INNOWax, respectively. Finally, we reported for the first time the enantiomeric distribution of P. rubra flowers, where the enantiomers (1R,5R)-(+)-α-pinene, (S)-(−)-limonene, (S)-(+)-Linalool, and (1S,2R,6R,7R,8R)-(+)-α-copaene were present as enantiomerically pure substances, whereas (S)-(+)-(E)-Nerolidol and (R)-(+)-(E)-Nerolidol were observed as scalemic mixtures. This study provides the first comprehensive and comparative aroma profile of Plumeria rubra cultivated in southern Ecuador and gave us a clue to the variability of P. rubra chemotypes depending on the harvesting time, which could be used for future quality control or applications in phytopharmaceutical and food industries. Full article
(This article belongs to the Special Issue Extraction, Composition and Comparison of Plant Volatile Components)
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