Characterization and Applications of Peculiar Genes and Recombinant Proteins and Possible Applications from Plants and Algae

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Molecular Biology".

Deadline for manuscript submissions: closed (10 September 2022) | Viewed by 8057

Special Issue Editors

Department of Biology, University of Naples “Federico II”, Via Cinthia, I-80126 Naples, Italy
Interests: crops; landraces; glucose metabolism; NGS; abiotic stress; algal biotechnology; synthetic biology
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Guest Editor
Consiglio Nazionale delle Ricerche, Istituto di Chimica Biomolecolare (CNR-ICB), Pozzuoli, Italy
Interests: microalgae; algal biotechnology; chloroplast transformation; recombinant proteins; extracellular vesicles; immune system
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
Department of Biology, University of Naples “Federico II”, Via Cinthia, 80126 Napoli, Italy
Interests: G6PDH; kinetics; nitrogen metabolism; protein folding; plant physiology; abiotic stress

Special Issue Information

Dear Colleagues, 

Nowadays, plants, crops, algae, and diatoms represent a critical, renewable, and sustainable resource. The full exploitation of hidden resources from these organisms still remains a challenge for plant researchers. A wide range of undiscovered proteins and/or alleles could be exploited for many applications. At the same time, well-known proteins and genes hide abilities to manipulate in different ways. 

This Special Issue is soliciting research papers and reviews that describe novel and innovative uses of proteins for plants and algae. Furthermore, paper focused on peculiar genes will be also welcomed. Researchers are encouraged to submit papers that explore both purification of proteins and/or identification of suitable alleles and to explore the potential application of these in different fields, namely industrial biotechnologies, crop improvement, life science, and others.

Dr. Simone Landi
Dr. Daniela Castiglia
Dr. Sergio Esposito
Guest Editors

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Keywords

  • plant biotechnologies
  • algal biotechnologies
  • central metabolism
  • fermentation
  • sugars
  • green factory
  • recombinant proteins.

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Published Papers (2 papers)

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Research

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23 pages, 9048 KiB  
Article
Removal of Methylene Blue and Congo Red Using Adsorptive Membrane Impregnated with Dried Ulva fasciata and Sargassum dentifolium
by Ahmed Labena, Ahmed E. Abdelhamid, Abeer S. Amin, Shimaa Husien, Liqaa Hamid, Gehan Safwat, Ayman Diab, Adil A. Gobouri and Ehab Azab
Plants 2021, 10(2), 384; https://doi.org/10.3390/plants10020384 - 17 Feb 2021
Cited by 30 | Viewed by 3074
Abstract
Biosorption is a bioremediation approach for the removal of harmful dyes from industrial effluents using biological materials. This study investigated Methylene blue (M. blue) and Congo red (C. red) biosorption from model aqueous solutions by two marine macro-algae, Ulva fasciata and Sargassum dentifolium, [...] Read more.
Biosorption is a bioremediation approach for the removal of harmful dyes from industrial effluents using biological materials. This study investigated Methylene blue (M. blue) and Congo red (C. red) biosorption from model aqueous solutions by two marine macro-algae, Ulva fasciata and Sargassum dentifolium, incorporated within acrylic fiber waste to form composite membranes, Acrylic fiber-U. fasciata (AF-U) and Acrylic fiber-S. dentifolium (AF-S), respectively. The adsorption process was designed to more easily achieve the 3R process, i.e., removal, recovery, and reuse. The process of optimization was implemented through one factor at a time (OFAT) experiments, followed by a factorial design experiment to achieve the highest dye removal efficiency. Furthermore, isotherm and kinetics studies were undertaken to determine the reaction nature. FT-IR and SEM analyses were performed to investigate the properties of the membrane. The AF-U membrane showed a significant dye removal efficiency, of 88.9% for 100 ppm M. blue conc. and 79.6% for 50 ppm C. red conc. after 240 min sorption time. AF-S recorded a sorption capacity of 82.1% for 100 ppm M. blue conc. after 30 min sorption time and 85% for 100 ppm C. red conc. after 240 min contact time. The membranes were successfully applied in the 3Rs process, in which it was found that the membranes could be used for five cycles of the removal process with stable efficiency. Full article
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Review

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16 pages, 1257 KiB  
Review
Advanced Applications for Protein and Compounds from Microalgae
by Daniela Castiglia, Simone Landi and Sergio Esposito
Plants 2021, 10(8), 1686; https://doi.org/10.3390/plants10081686 - 16 Aug 2021
Cited by 13 | Viewed by 3701
Abstract
Algal species still show unrevealed and unexplored potentiality for the identification of new compounds. Photosynthetic organisms represent a valuable resource to exploit and sustain the urgent need of sustainable and green technologies. Particularly, unconventional organisms from extreme environments could hide properties to be [...] Read more.
Algal species still show unrevealed and unexplored potentiality for the identification of new compounds. Photosynthetic organisms represent a valuable resource to exploit and sustain the urgent need of sustainable and green technologies. Particularly, unconventional organisms from extreme environments could hide properties to be employed in a wide range of biotechnology applications, due to their peculiar alleles, proteins, and molecules. In this review we report a detailed dissection about the latest and advanced applications of protein derived from algae. Furthermore, the innovative use of modified algae as bio-reactors to generate proteins or bioactive compounds was discussed. The latest progress about pharmaceutical applications, including the possibility to obtain drugs to counteract virus (as SARS-CoV-2) were also examined. The last paragraph will survey recent cases of the utilization of extremophiles as bio-factories for specific protein and molecule production. Full article
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