Genetics and Epigenetics in the Brassicaceae

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Systems and Synthetic Biology".

Deadline for manuscript submissions: closed (31 August 2024) | Viewed by 2699

Special Issue Editors


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Guest Editor
Graduate School of Agricultural Science, Kobe University, Kobe 6578501, Japan
Interests: epigenetics; hybrid vigor; heterosis; vernalization; Brassica
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
Graduate School of Agricultural Science, Kobe University, Rokkodai, Nada-ku, Kobe 657-8501, Japan
Interests: heterosis; epigenetics; genotype to phenotype; brassica; plant tissue culture
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
Graduate School of Life Sciences, Tohoku University, Sendai 980-8577, Japan
Interests: pollen-stigma recognition; self-incompatibility; genetics; Brassica
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Brassicaceae is a diverse family of angiosperms containing 338 genera and 3709 species, including the model plant Arabidopsis thaliana. The genus Brassica has six major species (Brassica rapa, B. nigra, B. oleracea, B. juncea, B. napus, and B. carinata) that include vegetables (Chinese cabbage, cabbage, napa cabbage, cauliflower, broccoli, kale, pak choi, mizuna, komatsuna, Brussels sprouts, kohlrabi, rutabaga, turnip), oilseeds (canola, rapeseed), condiment (mustard), ornamentals (ornamental cabbage and kale), and fodder. Plant species in the Brassica genus are of interest to researchers because of their agricultural importance. Brassica species are also an excellent representative of different polyploidization events such as whole-genome triplication and allotetraploidization; therefore, they are considered ideal for polyploidization studies. Advanced sequencing technologies accelerated genetic studies in the Brassicaceae, and genomic loci or causative genes associated with specific phenotypes, natural variation, or agricultural traits were identified. Genome editing allows studying the functions of genes.

In addition to genetic studies, epigenetic studies have also advanced. Epigenetic regulators including DNA methylation, histone modifications, chromatin remodeling, and histone posttranslational modifications can actively control gene expression without changing the DNA sequence. Non-coding RNAs can also engage in controlling the gene functions in various aspects. Advanced sequencing technologies provide benefits for researcher, allowing them to examine the epigenetic states or identify non-coding RNAs at the whole-genome level.

In this Special Issue, we aim to accumulate a wide understanding of current genetic and epigenetic studies in the Brassicaceae, especially in Brassica crops. Therefore, we welcome the submission of genetic and epigenetic studies related to biotic stress, abiotic stress, heterosis, breeding, agronomic traits, genetic variability, etc. in Brassicaceae. Articles on the integrated analysis of different omics studies such as genomics, transcriptomics, proteomics, metabolomics, and epigenomics are also welcome.

Dr. Ryo Fujimoto
Dr. Yoshinobu Takada
Dr. Hasan Mehraj
Guest Editors

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Keywords

  • heredity abiotic stress
  • agronomic traits biotic stress
  • DNA marker
  • DNA methylation
  • epigenetics
  • flowering time
  • genetic diversity
  • heterosis
  • histone modification
  • male sterility
  • marker-assisted selection
  • Non-coding RNAs
  • QTL analysis
  • self-incompatibility
  • transcriptomics
  • vernalization

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Published Papers (1 paper)

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Research

16 pages, 3462 KiB  
Article
The Highly Embryogenic Brassica napus DH4079 Line Is Recalcitrant to Agrobacterium-Mediated Genetic Transformation
by Antonio Calabuig-Serna, Ricardo Mir, Rosa Porcel and Jose M. Seguí-Simarro
Plants 2023, 12(10), 2008; https://doi.org/10.3390/plants12102008 - 17 May 2023
Cited by 2 | Viewed by 1939
Abstract
Brassica napus is a species of high agronomic interest, used as a model to study different processes, including microspore embryogenesis. The DH4079 and DH12075 lines show high and low embryogenic response, respectively, which makes them ideal to study the basic mechanisms controlling embryogenesis [...] Read more.
Brassica napus is a species of high agronomic interest, used as a model to study different processes, including microspore embryogenesis. The DH4079 and DH12075 lines show high and low embryogenic response, respectively, which makes them ideal to study the basic mechanisms controlling embryogenesis induction. Therefore, the availability of protocols for genetic transformation of these two backgrounds would help to generate tools to better understand this process. There are some reports in the literature showing the stable transformation of DH12075. However, no equivalent studies in DH4079 have been reported to date. We explored the ability of DH4079 plants to be genetically transformed. As a reference to compare with, we used the same protocols to transform DH12075. We used three different protocols previously reported as successful for B. napus stable transformation with Agrobacterium tumefaciens and analyzed the response of plants. Whereas DH12075 plants responded to genetic transformation, DH4079 plants were completely recalcitrant, not producing any single regenerant out of the 1784 explants transformed and cultured. Additionally, an Agrobacterium rhizogenes transient transformation assay was performed on both lines, and only DH12075, but no DH4079 seedlings, responded to A. rhizogenes infection. Therefore, we propose that the DH4079 line is recalcitrant to Agrobacterium-mediated transformation. Full article
(This article belongs to the Special Issue Genetics and Epigenetics in the Brassicaceae)
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